Abstract: The experiment was conducted to set up the acute diarrhea model of the mice. 30 mice were randomly divided into 3 groups named as following: Model group 1, Model group 2 and control group. Each of the mice were all fed 1 g/mL basal diet, respectively plus 0.2 mL Folium Sennae, 0.3 mL Folium Sennae and 0.2 mL normal saline. Each of the three drug-delivery were processed twice every day. The duration was five days. The defecation of the mice was observed and the indexes of loose stool rate, degree of diarrhea, diarrhea index were tested. Blood of half of the mice in every group were collected through the eye socket at the sixth day after starting the experiment, which were used to measure blood sugar and triglycerides. All of the mice with blood collected were killed with their necks severed for collecting the samples of ileum and cecum which were used to make pathological sections of hematoxylin and eosin. The results showed that both levels of the two group intake Folium Sennae have developed the mice acute diarrhea model（P<0.05）and had no significant effect on blood sugar of the mice. The blood triglycerides of the mice intake higher dose Folium Sennae significantly decreased（P<0.01）. Neither groups of taking Folium Sennae had obvious necrosis or inflammation of intestinal mucous. The result showed that the two doses of Folium Sennae can establish acute diarrhea model successfully, much better with higher dose group（0.3 mL）.

Key words: mice, acute diarrhea model, Folium Sennae, diarrhea index