犬子宫蓄脓中绿脓杆菌分离鉴定及耐药性分析

doi:10.19640/j.cnki.jtau.2018.03.010

Journal of Tianjin Agricultural University ›› 2018, Vol. 25 ›› Issue (3): 47-51.doi: 10.19640/j.cnki.jtau.2018.03.010

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Isolation, identification and susceptibility analysis of Bacillus pyocyaners from a canine pyometra

ZHU Kun-yuan, WU Lan, LIU Yan-fei^{Corresponding Author}, YANG Jian-de^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2018-03-26 Online:2018-09-20 Published:2019-11-12

Abstract

Abstract: In order to clarify the causes of the fester disease in companion animals, the study involved isolation, identification and drug susceptibility analysis of pathogenic bacteria from an 11-year-old female dog’s uterus who were admitted to the veterinary hospital of Tianjin Agricultural University. According to the results of colony morphology, smear staining microscopy, physiological and biochemical reaction and 16S rRNA gene analysis, the bacteria was identified as a Bacillus pyocyaners. Susceptibility analysis showed that the isolate is highly sensitive to aztreonam, cefdinir, cefotaxime, cefepime, ceftazidime, ofloxacin, streptomycin, kanamycin, piperacillin and tobramycin. Moreover, this isolate is moderately sensitive to ceftriaxone, cefuroxime and cefoxitin, but resistant to ampicillin, cefazolin, cefazine and medemycin. The research results provided references for controlling infection caused byBacillus pyocyaners.

Key words: Bacillus pyocyaners, isolation and identification, 16S rRNA gene, susceptibility analysis

CLC Number:

S852.611

ZHU Kun-yuan, WU Lan, LIU Yan-fei, YANG Jian-de. Isolation, identification and susceptibility analysis of Bacillus pyocyaners from a canine pyometra[J]. Journal of Tianjin Agricultural University, 2018, 25(3): 47-51.

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Isolation, identification and susceptibility analysis of Bacillus pyocyaners from a canine pyometra

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