饲料不同糖脂比对鲤鱼生长、免疫及抗氧化相关酶活性的影响

天津农学院学报 ›› 2016, Vol. 23 ›› Issue (1): 5-5.

饲料不同糖脂比对鲤鱼生长、免疫及抗氧化相关酶活性的影响

陈天翔¹, 曲木¹, 吕春双², 李嫦娥², 白东清¹, 孙金辉¹, 乔秀亭¹, 程镇燕^{1,通信作者}

1. 天津农学院水产学院,天津市水产生态及养殖重点实验室,天津 300384;
2. 武清区畜牧水产业发展服务中心,天津 300380

收稿日期:2015-07-30 出版日期:2016-03-20 发布日期:2019-10-14
通讯作者: 程镇燕（1981-）,女,山东泰安人,副教授,博士,主要从事水产动物营养与饲料的研究。E-mail：chengzhenyan2005@126.com。
作者简介:陈天翔（1990-）,男,浙江宁波人,硕士在读,主要从事水产动物营养与饲料的研究。E-mail：61832344@qq.com。曲木（1990-）,女,山东烟台人,硕士在读,主要从事水产动物营养与饲料的研究。E-mail：310901543@qq.com。
基金资助:
天津市大学生创新创业训练计划项目“饲料不同糖脂比对鲤鱼生长、免疫及抗氧化相关酶活性的影响”（201410061168）; 天津市科技支撑计划项目“渔用低氮环保饲料的研究与应用”（13ZCZDNC00900）; 天津市应用基础与前沿技术研究计划项目“鲤鱼糖代谢关键酶的基因表达及调控机理”（14JCQNJC15100）; 天津市高等学校创新团队资助项目“现代水产生态健康养殖”（TD12-5018）; 天津市高等学校“中青年骨干创新人才培养计划”资助项目（无编号）

Effects of Different Dietary Carbohydrate-to-lipid Ratio on Growth, Immune Ability and Antioxidant Enzymes Activity of Carp

CHEN Tian-xiang¹, QU Mu¹, LÜ Chun-shuang², LI Chang-e², BAI Dong-qing¹, SUN Jin-hui1, QIAO Xiu-ting¹, CHENG Zhen-yan^{1,Corresponding Author}

1. Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
2. Animal Husbandry and Fishery Service Center, Wuqing District, Tianjin 300380, China

Received:2015-07-30 Online:2016-03-20 Published:2019-10-14

摘要/Abstract

摘要： 为研究不同糖脂比对鲤鱼生长及免疫力的影响,选取平均体重为（44.45±1.6）g的鲤鱼 1 050尾,随机分成5组（每组3个重复,每个重复70尾）,分别饲喂糖脂比为5.2、6.9、9.7、14.8、27.8的饲料,进行为期63 d的摄食生长试验。结果表明：摄食饲料糖脂比为6.9的鲤鱼增重率和特定生长率最好,且饲料系数最低,显著优于其他处理组（P<0.05）。随着糖脂比的升高,血清超氧化物歧化酶活性呈先上升后下降的趋势,且糖脂比为6.9时,达到最高,显著高于糖脂比为14.8和27.8的处理组（P<0.05）;血清过氧化氢酶活性呈下降趋势;糖脂比为9.7时,血清及肝胰脏丙二醛含量最低;在肝胰脏中糖脂比为27.8时,谷草转氨酶、谷丙转氨酶活性最低;碱性磷酸酶活性则呈先上升后下降的趋势。由此可见,在本试验条件下,饲料糖脂比为6.9时鲤鱼的生长最好,且免疫力和抗氧化能力处于较佳状态。

关键词: 鲤鱼, 糖脂比, 生长, 免疫力, 抗氧化

Abstract: A feeding trial was conducted to explore the effect of different dietary carbohydrate-to-lipid ratio on growth and immune ability of carp. 1 050 carp were randomly divided into 5 groups. Each group has three replicate and each replicate has 70 fish. Five diets were formulated with carbohydrate-to-lipid ratio of 5.2, 6.9, 9.7, 14.8, 27.8. The feeding trial lasted for 63 day. Results showed that dietary carbohydrate-to-lipid (CHO/LIP) ratio level remarkably affected the growth performance of carp (P<0.05). At dietary CHO/LIP ratio of 6.9, the weight gain, special growth ratio got the maximum, while the feed conversion ratio got the minimum. With the increasing of dietary CHO/LIP ratio level, antioxidant capacity of carp first raised and then decreased. At dietary CHO/LIP ratio of 6.9, the activity of superoxide dismutase (SOD) in serum reached the highest level, which was significantly higher than that in fish fed diet with CHO/LIP ratio of 14.8 and 27.8. While the malondialdehyde (MDA) content in serum and hepatopancreas got the minimum when the dietary CHO/LIP ratio was 9.7. Catalase (CAT) activity in serum significantly decreased with the increasing dietary CHO/LIP ratio. At the same time, the glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) activity got the minimum at 27.8, and the alkaline phosphatase (AKP) first raised and then went down. Considered the growth and antioxidant enzymes activity, the optimal dietary CHO/LIP ratio of carp was 6.9.

Key words: carp, carbohydrate-to-lipid ratio, growth, immunity, antioxidant

中图分类号:

S963.71

陈天翔, 曲木, 吕春双, 李嫦娥, 白东清, 孙金辉, 乔秀亭, 程镇燕. 饲料不同糖脂比对鲤鱼生长、免疫及抗氧化相关酶活性的影响[J]. 天津农学院学报, 2016, 23(1): 5-5.

CHEN Tian-xiang, QU Mu, LÜ Chun-shuang, LI Chang-e, BAI Dong-qing, SUN Jin-hui1, QIAO Xiu-ting, CHENG Zhen-yan. Effects of Different Dietary Carbohydrate-to-lipid Ratio on Growth, Immune Ability and Antioxidant Enzymes Activity of Carp[J]. Journal of Tianjin Agricultural University, 2016, 23(1): 5-5.

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