白藜芦醇对ZEN诱导的猪小肠上皮细胞损伤的作用效果研究

doi:10.19640/j.cnki.jtau.2023.03.009

天津农学院学报 ›› 2023, Vol. 30 ›› Issue (3): 47-52.doi: 10.19640/j.cnki.jtau.2023.03.009

白藜芦醇对ZEN诱导的猪小肠上皮细胞损伤的作用效果研究

韩婧娥, 王欢欢, 李鹏程, 佘福泽, 黎肖昱, 秦顺义^通信作者

天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室,天津 300392

收稿日期:2022-04-25 出版日期:2023-06-30 发布日期:2023-09-06
通讯作者: 秦顺义（1979—）,男,副教授,博士,主要从事动物营养代谢病与中毒病方面研究。E-mail：qinshunyi@163.com。
作者简介:韩婧娥（1998—）,女,硕士在读,研究方向为临床兽医学。E-mail：3155409957@qq.com。
基金资助:
天津市大学生创新创业计划训练项目（202110061048）; 天津市生猪产业技术体系创新团队（ITTPRS2023006）

Effects of resveratrol on zearalenone induced injury of porcine intestinal epithelial cells

Han Jing’e, Wang Huanhuan, Li Pengcheng, She Fuze, Li Xiaoyu, Qin Shunyi^{Corresponding Author}

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China

Received:2022-04-25 Online:2023-06-30 Published:2023-09-06

摘要/Abstract

摘要： 试验旨在探究白藜芦醇对玉米赤霉烯酮（ZEN）诱导的猪小肠上皮细胞（IPEC-J2细胞）损伤的影响。试验分组为：C组（对照组）、Z组（ZEN浓度25 µg/mL）、ZR1组（25 µg/mL ZEN+10 µmol/L白藜芦醇）、ZR2组（25 µg/mL ZEN+20 µmol/L白藜芦醇）、ZR3组（25 µg/mL ZEN+30 µmol/L白藜芦醇）;测定细胞活力、细胞乳酸脱氢酶（LDH）活性、抗氧化酶水平以及Caspase 3、Caspase 9、Bcl-2和bax等凋亡相关基因mRNA表达水平。试验结果显示：Z组细胞活力、SOD活性T-AOC水平和Bcl-2基因mRNA表达水平极显著低于C组（P<0.01）,ZR2组和ZR3组细胞活力、SOD活性、T-AOC水平和Bcl-2基因mRNA表达水平极显著或显著高于Z组和ZR1组（P<0.01或P<0.05）;Z组细胞LDH活性、MDA水平、Capase-3、Capase-9和Bax基因mRNA表达水平极显著高于C组（P<0.01）,ZR2组和ZR3组细胞LDH活性、MDA水平、Capase-3、Capase-9和Bax基因mRNA表达水平极显著或显著低于Z组和ZR1组（P<0.01或P<0.05）,ZR1组细胞LDH活性和Bax基因mRNA表达水平极显著低于Z组（P<0.01）。结果表明,白藜芦醇能够缓解ZEN诱导的IPEC-J2细胞的细胞活力、LDH活性、抗氧化功能和凋亡相关基因mRNA表达水平的改变。

关键词: 白藜芦醇, 玉米赤霉烯酮, 猪小肠上皮细胞, 细胞活力, 乳酸脱氢酶活性, 抗氧化功能, 凋亡基因

Abstract: The purpose of this study was to investigate the effect of resveratrol on the injury of porcine small intestinal epithelial cells（IPEC-J2 cells）induced by zearalenone（Zen）. The experimental groups were: Group C（control group）, group Z（25 µg/mL Zen）, group ZR1（25 µg/mL Zen+10 µmol/L resveratrol）, group ZR2（25 µg/mL Zen+20 µmol/L resveratrol）, group ZR3（25 µg/mL Zen+30 µmol/L resveratrol）. The cell viability, the activity of lactate dehydrogenase（LDH）, the level of antioxidant enzymes and the mRNA expression of apoptosis related genes such as caspase 3, caspase 9, Bcl-2 and Bax were measured. The results showed that the cell viability, SOD activity, T-AOC level and Bcl-2 gene mRNA expression level in group Z were significantly lower than those in group C（P<0.01）. The cell viability, SOD activity, T-AOC level and Bcl-2 gene mRNA expression level in group ZR2 and ZR3 were significantly higher than those in group Z or group ZR1（P<0.01 or P<0.05）. The LDH activity, MDA level, Capase-3, Capase-9 and Bax gene mRNA expression levels in group Z were significantly higher than those in group C（P<0.01）. The LDH activity, MDA level, Capase-3, Capase-9 and Bax gene mRNA expression levels in group ZR2 and ZR3 were lower or significantly lower than those in group Z or group ZR1（P<0.01 or P<0.05）. The LDH activity and Bax gene mRNA expression levels in ZR1 group were lower than those in group Z（P<0.01）. The results indicated that resveratrol could alleviate the changes of cell viability, LDH activity, antioxidant function and apoptosis related gene mRNA expression of IPEC-J2 cells induced by Zen.

Key words: resveratrol, zearalenone, porcine intestinal epithelial cells, cell viability, lactate dehydrogenase activity, antioxidant function, apoptosis gene

中图分类号:

S816.7

韩婧娥, 王欢欢, 李鹏程, 佘福泽, 黎肖昱, 秦顺义. 白藜芦醇对ZEN诱导的猪小肠上皮细胞损伤的作用效果研究[J]. 天津农学院学报, 2023, 30(3): 47-52.

Han Jing’e, Wang Huanhuan, Li Pengcheng, She Fuze, Li Xiaoyu, Qin Shunyi. Effects of resveratrol on zearalenone induced injury of porcine intestinal epithelial cells[J]. Journal of Tianjin Agricultural University, 2023, 30(3): 47-52.

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http://xuebao.tjau.edu.cn/CN/Y2023/V30/I3/47

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白藜芦醇对ZEN诱导的猪小肠上皮细胞损伤的作用效果研究

Effects of resveratrol on zearalenone induced injury of porcine intestinal epithelial cells

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