锦带花组培快繁体系的建立

doi:10.19640/j.cnki.jtau.2023.03.003

Journal of Tianjin Agricultural University ›› 2023, Vol. 30 ›› Issue (3): 12-15.doi: 10.19640/j.cnki.jtau.2023.03.003

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Establishment of tissue culture and rapid propagation system of Weigela florida

Li Deping, Xu Dingfan, He Yuanqin, Yang Senru, Huang Junxuan^{Corresponding Author}, Liu Yanjun

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China

Received:2022-07-06 Online:2023-06-30 Published:2023-09-06

Abstract

Abstract: The stem segment of axillary bud was used as explants to explore the best method of explants disinfection, the best medium for primary generation and the best medium for proliferation, and to establish the tissue culture and rapid propagation system of Weigela florida. The results showed that the best disinfection method for explants was 2% sodium hypochlorite solution for 10 min, and then inoculated into MS medium supplemented with 125 mg/L benzylpenicillin. The contamination rate of explants was only 1.67%, and germination rate was 98.3%. The optimal medium for primary culture was WPM. The average plant height and stem diameter of tissue culture seedlings were 5.58 cm and 1.7 cm respectively. The optimal medium for proliferation was WPM+1.0 mg/L BA+0.5 mg/L IAA, the proliferation coefficient was 8.0, the height of bud was 1.7 cm, and the growth potential was strong without vitrification. This study can provide effective technical support for the factory production and breeding of Weigela florida.

Key words: Weigela florida, stem segment, basic medium, multiplication culture

CLC Number:

S685.99

Li Deping, Xu Dingfan, He Yuanqin, Yang Senru, Huang Junxuan, Liu Yanjun. Establishment of tissue culture and rapid propagation system of Weigela florida[J]. Journal of Tianjin Agricultural University, 2023, 30(3): 12-15.

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Establishment of tissue culture and rapid propagation system of Weigela florida

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