栒子试管苗露地环境中瓶内生根和直接入地移栽研究

doi:10.19640/j.cnki.jtau.2018.04.001

Journal of Tianjin Agricultural University ›› 2018, Vol. 25 ›› Issue (4): 1-5.doi: 10.19640/j.cnki.jtau.2018.04.001

• Researches and Scientific Notes • Next Articles

Studies on in vitro rooting and direct transplanting into ground of Cotoneaster hjelmqvistii plantlets in vitro in field environment

WANG Ran¹, SHI Hai-dong¹, CHAI Ci-jiang^{1,Corresponding Author}, WEI Rao-pei¹, SONG Qiu-yue¹, SHI Yan-shan¹, LUO Jian-xia¹, ZHANG Yan²

1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China;
2. Inner Mongolia Tongliao Horqin District Agricultural Technology Extension Center, Tongliao 028000, Inner Mongolia Autonomous Region, China

Received:2018-08-18 Online:2018-12-20 Published:2019-11-12

Abstract

Abstract: The aim of this study was to research on in vitro rooting and transplanting of Cotoneaster hjelmqvistii plantlets in vitro in field environment. Three shading methods（shading with two layers of sunshade net, three layers of sunshade net or tree shade）were used for in vitro rooting of C. hjelmqvistii plantlets in field environment in last ten days of April, and transplanting experiments were carried out with two preserve moisture cover methods in two periods. The results showed that there was no significant difference in the effect of root culture among the three shading methods, and the rooting rate of 89.7%-95.9% were obtained meanwhile the plantlets grew normally. The closure function of the stomata in treatment of shading with two layers of sunshade net was obviously stronger than that of the other two shading methods. There was no significant difference in the survival rate of the plantlet transplanted between both preserve moisture cover methods of the fully cover and the cover with top opening; the survival rate of transplanted plantlets in June 1st and June 27th had no significant difference, and the survival rate was all up to 97.5%-100.0%. The results can provide a basis for reducing the cost of C. hjelmqvistii plantlets in vitro and promoting the tissue culture rapid propagation technique application to reproduction.

Key words: Cotoneaster hjelmqvistii plantlet in vitro, field environment, in vitro rooting, transplanting

CLC Number:

WANG Ran, SHI Hai-dong, CHAI Ci-jiang, WEI Rao-pei, SONG Qiu-yue, SHI Yan-shan, LUO Jian-xia, ZHANG Yan. Studies on in vitro rooting and direct transplanting into ground of Cotoneaster hjelmqvistii plantlets in vitro in field environment[J]. Journal of Tianjin Agricultural University, 2018, 25(4): 1-5.

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References

[1] 骆建霞,马莉,柴慈江,等. 干旱胁迫对海姆维斯蒂栒子生长及丙二醛和脯氨酸含量的影响[J]. 天津农业科学,2009,15(1):8-11.
[2] 骆建霞,申屠雅瑾,张津华,等. 盐胁迫对海姆维斯蒂栒子生长及丙二醛和脯氨酸含量的影响[J]. 天津农学院学报,2008,15(4):8-11.
[3] 柴慈江,史燕山,骆建霞,等. 栒子的组织培养与快速繁殖[J]. 植物生理学通讯,2006,42(3):484.
[4] 柴慈江,史燕山,骆建霞,等. 栒子试管苗蛭石支撑生根培养及炼苗移栽研究[J]. 安徽农业科学,2009,37(17):7860-7861,7871
[5] 柴慈江,史燕山,骆建霞,等. 栒子试管苗的土壤支撑生根培养和带坨移栽[J]. 安徽农业科学,2010,38(12):6116-6117,6133
[6] 梁发辉,柴慈江,何丹,等. 降低栒子试管苗培养成本的方法研究[J]. 北方园艺,2013(17):107-109.
[7] 柴慈江,史燕山,骆建霞,等. 土壤做培养基支撑物对栒子试管苗生根培养的影响[J]. 天津农学院学报,2013,20(2):18-21.
[8] 卢兴霞,柴慈江,张婷,等. 栒子试管苗玻璃化影响因素的研究[J]. 北方园艺,2014(18):103-106.
[9] 柴慈江,祁志彬,汪强强,等. 移栽时期和环境条件对栒子试管苗成活与生长的影响[J]. 天津农学院学报,2017,24(2):22-24,29.
[10] 柴慈江,曹海鹏,王琼,等. 不同温室培养条件对栒子微枝试管内生根和成活的影响[J]. 天津农学院学报,2015,22(2):11-15.
[11] 宋秋月,韦绕培,柴慈江,等. 露地与培养室环境对栒子微枝试管内生根与成活的影响[J]. 天津农学院学报,2018,25(2):1-4.
[12] 陈佰鸿,李新生,曹孜义,等. 一种用透明胶带粘取叶片表皮观察气孔的方法[J]. 植物生理学通讯,2004,40(2):215-218.
[13] 柴慈江. 用于试管苗带坨移栽的提苗片:ZL2008200 75225.3[P].2009-05-13.
[14] 彭立新. 园艺植物生物技术[M]. 北京:中国农业出版社,2014.
[15] 韩会会,符玲巧,柴慈江,等. 温室培养条件对无花果微枝试管内生根与成活的影响[J]. 天津农学院学报,2016,23(1):23-27.
[16] 杨广艳,王坤英,柴慈江,等. 环境条件对珠美海棠微枝试管内生根与成活的影响[J]. 天津农学院学报,2017,24(4):15-18.
[17] 韦梅琴,唐楠,魏国良,等. 香石竹组培苗与温室苗叶表皮气孔特征比较[J]. 青海大学学报,2016,34(5):20-24.
[18] 陈影,郑枫,吴坤林,等. 魔帝兜兰试管苗移栽过程中叶片气孔变化及光合特性[J]. 植物生理学报,2017,53(12):2137-2146.
[19] 柴慈江,卢兴霞,苏卫国,等. 珠美海棠试管苗的土壤支撑生根培养和带坨移栽[J]. 植物生理学通讯,2010,46(1):33-36.
[20] 柴慈江,汪强强,骆建霞,等. 矮溲疏试管苗的土壤支撑生根培养和带坨移栽[J]. 天津农学院学报,2014,21(4):22-25.
[21] 袁惠君,宋占午,丁兰,等. 人参果试管苗与移栽成活苗气孔行为的比较[J]. 西北师范大学学报(自然科学版),2001,37(4):89-92.
[22] 夏阳,罗新书. 几种果树水分生理指标的比较研究[J]. 落叶果树,1993(1):1-8.
[23] 解晓红,解红娥,李江辉,等. 地黄脱毒试管苗大田直接移栽技术研究[J]. 中药材,2015,38(2):231-233.

Studies on in vitro rooting and direct transplanting into ground of Cotoneaster hjelmqvistii plantlets in vitro in field environment

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