樱桃李组织培养快速繁殖技术研究

doi:10.19640/j.cnki.jtau.2019.03.001

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (3): 1-6.doi: 10.19640/j.cnki.jtau.2019.03.001

• Researches and Scientific Notes • Next Articles

Study on tissue culture and rapid propagation of Prunus cerasifera

HE Xiang-su, CHAI Ci-jiang, GUO Jing, ZHU Lin, WANG Xue-tong, FENG Tao

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-03-21 Online:2019-09-30 Published:2019-09-30

Abstract

Abstract: The stem sections were taken from new shoots of Prunus cerasifera which grew in field on May 15^th and July 9^th in 2018 respectively and were sterilized with alcohol and sodium hypochlorite. The sterile stem buds were proliferated on medium containing different concentrations of IBA, rooted in soil supporting medium, and then the plantlet were transplanted. The results showed that there was no obvious difference in the sterilization effect of the samples at different time, and the contamination rate was less than 10% and the germination rate was 100%. There was no obvious different effects between washing and no-washing by sterilized water after soaked in alcohol on sterilization. The propagation coefficient of the plantlet of Prunus cerasifera was 4.2 in 1/2MS medium added with 0.4 mg/L IBA when cultured for 60 day. The rooting rate of the plantlet of Prunus cerasifera cultured in soil supporting medium was 68.8%, which was obviously higher than that of the plantlet cultured in agar medium. The survival rate of the plantlet of Prunus cerasifera transplanted in soil supporting medium was 75.0%, which was obviously higher than that of plantlet in agar medium（33.3%）. These results can provide basis for improving rapid propagation technique of Prunus cerasifera.

Key words: Prunus cerasifera, sterilizing, plantlet in vitro, multiplication, rooting

CLC Number:

HE Xiang-su, CHAI Ci-jiang, GUO Jing, ZHU Lin, WANG Xue-tong, FENG Tao. Study on tissue culture and rapid propagation of Prunus cerasifera[J]. Journal of Tianjin Agricultural University, 2019, 26(3): 1-6.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2019.03.001

http://xuebao.tjau.edu.cn/EN/Y2019/V26/I3/1

References

[1] 关海军,崔大方,许正,等. 中国天山野果林种子植物组成及资源状况分析[J]. 植物资源与环境学报,2003,12(2):39-45.
[2] 刁永强,许正,陈淑英,等. 新疆野生樱桃李种质资源研究现状及保护对策[J]. 中国野生植物资源,2017,36(3):57-61.
[3] 车凤斌,何琼,苏馨华,等. 樱桃李营养成分分析及产业化发展相关问题的探讨[J]. 新疆农业科学,2007,44(1):59-62.
[4] 张士康,肖正春,张广伦,等. 樱桃李与蓝莓果的营养价值[J]. 中国野生植物资源,2004,23(3):1-3.
[5] 张静茹,孙海龙,陆致成,等. 野生樱桃李(Prunus cerasifera)果实多酚多样性分析[J]. 果树学报,2017(5):567-575.
[6] 王玮,沈静,姚军. 新疆野生樱桃李果皮中花色苷纯化工艺及体外抗氧化活性[J]. 食品科技,2018,43(2):247-250.
[7] 刘伟,腊萍,杨如箴,等. 野生樱桃李清除DPPH自由基能力及抑制α-葡萄糖苷酶活性[J]. 江苏农业科学, 2017,45(17):183-185.
[8] 刘伟,刘天琦,葛豫炜,等. UPLC-UV-QDa联用系统快速分析野生樱桃李叶化学成分[J]. 食品与机械,2018,34(9):96-99,134.
[9] 单奇,王春梅,王献亮. 乌昌地区野生樱桃李引种繁殖及应用研究[J]. 中国园艺文摘,2017(11):40-41.
[10] 彭立新. 园艺植物生物技术[M]. 北京:中国农业出版社,2014.
[11] 刘崇琪,陈学森,吴传金,等. 新疆野生樱桃李(Prunus cerasifera)茎段与叶片培养及其植株再生[J]. 果树学报,2008,25(1):49-53.
[12] 李海臣,兰士波,罗旭. 樱桃李茎段离体培养与快速繁殖技术[J]. 中国林副特产,2011(3):14-16.
[13] 浦艳吉. 李茎尖离体培养与植株再生的研究[D]. 武汉:华中农业大学,2008.
[14] 侍艺,柴慈江,冯涛,等. 樱桃李休眠芽提早萌发及茎段灭菌培养研究[J]. 天津农学院学报,2018,25(2):24-29.
[15] 巩振辉,申书兴. 植物组织培养[M]. 2版. 北京:化学工业出版社,2013.
[16] 沈海龙. 植物组织培养[M]. 北京:中国林业出版社,2005.
[17] 徐志威,杜保伟,熊明国. 桃豫农矮砧1号外植体灭菌方法的研究[J]. 园艺与种苗,2014(12):33-34,37.
[18] 岑湘涛,沈伟. 外植体选择和灭菌对杧果组织培养影响的初步研究[J]. 安徽农学通报,2016,22(2):16-17.
[19] 李畅,付建敏,王森,等. 阳丰甜柿组织培养外植体的选择与灭菌[J]. 经济林研究,2016,34(1):158-163.
[20] 孙朝晖,曾春凤,赵玉芬,等. 抗枣疯病婆枣组织培养外植体灭菌时间试验[J]. 河北林业科技,2004(2):7-8.
[21] 延志莲,陈宗礼,薛皓,等. 大木枣休眠枝水培芽及其离体培养芽诱导的研究[J]. 延安大学学报(自然科学版),2000,19(1):66-71.
[22] 戴彩虹,马绍英,李胜,等. 山葡萄‘双红’和‘双优’的试管快繁研究[J]. 甘肃农业大学学报,2014,49(4):63-68.
[23] 王艳,彭建营. ‘赞皇大枣’增殖培养研究[J]. 中国农学通报,2011,27(16):210-214.
[24] 柴慈江,卢兴霞,苏卫国,等. 珠美海棠试管苗的土壤支撑生根培养和带坨移栽[J]. 植物生理学通讯,2010,46(1):33-36.
[25] 柴慈江,史燕山,骆建霞,等. 栒子试管苗的土壤支撑生根培养和带坨移栽[J]. 安徽农业科学,2010,38(12):6116-6117,6133.
[26] 柴慈江,曹海鹏,王琼,等. 不同温室培养条件对栒子微枝试管内生根和成活的影响[J]. 天津农学院学报,2015,22(2):11-15.
[27] 韩会会,符玲巧,柴慈江,等. 温室培养条件对无花果微枝试管内生根与成活的影响[J]. 天津农学院学报,2016(1):23-27.
[28] 杨广艳,王坤英,柴慈江,等. 环境条件对珠美海棠微枝试管内生根与成活的影响[J]. 天津农学院学报,2017,24(4):17-18.
[29] 宋秋月,韦绕培,柴慈江,等. 露地与培养室环境对栒子微枝试管内生根与成活的影响[J]. 天津农学院学报,2018,25(2):1-4.
[30] 王然,时海东,柴慈江,等. 栒子试管苗露地环境中瓶内生根和直接入地移栽研究[J]. 天津农学院学报,2018,25(4):1-5.

Study on tissue culture and rapid propagation of Prunus cerasifera

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Lu Xingxia, Yao Tongliang, Chai Cijiang, Shi Haidong. Effect of shading way and culture period in outdoor environment on rooting in vitro of Malus Zumi micro-shoots [J]. Journal of Tianjin Agricultural University, 2023, 30(3): 1-6.
[2]	Li Deping, Xu Dingfan, He Yuanqin, Yang Senru, Huang Junxuan, Liu Yanjun. Establishment of tissue culture and rapid propagation system of Weigela florida [J]. Journal of Tianjin Agricultural University, 2023, 30(3): 12-15.
[3]	Huang Jieping, Chai Cijiang, Xia Rui, Dong Yuanyuan, Cen Fen. Study on rooting of North American Begonia micro shoot [J]. Journal of Tianjin Agricultural University, 2022, 29(1): 28-32.
[4]	Xia Rui, Chai Cijiang, Huang Jieping, Dong Yuanyuan, Cen Fen. Effects of stem section, sugar-free medium and environment on rooting of Prunus cerasifera Ehrh. plantlet in vitro [J]. Journal of Tianjin Agricultural University, 2021, 28(4): 15-18.
[5]	Dong Yuanyuan, Chai Cijiang, Xia Rui, Huang Jieping, Cen Fen. Effects of environment, stem section state and sugar-free medium on rooting of grape plantlet in vitro cultured in soil supporting medium [J]. Journal of Tianjin Agricultural University, 2021, 28(1): 17-20.
[6]	YE Xia, SUN Shi-hai, GUO Ru, HUA Ren-rui. Effect of concentrations of ZT and IBA on multiplication in vitro of five blueberry varieties [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 14-17.
[7]	GUO Jing, CHAI Ci-jiang, SHI Yan-shan, LUO Jian-xia, JIANG Wen. Micro shoot proliferation and rooting of apple rootstock G.11 [J]. Journal of Tianjin Agricultural University, 2019, 26(4): 38-42.
[8]	CHAI Ci-jiang, WANG Kun-ying, ZHANG Wen-wen, LIU Chun-peng, WEI Wei, ZOU Hang. Effect of sterilizing method and concentration of ZT on blueberry plantlet in Vitro proliferation [J]. Journal of Tianjin Agricultural University, 2019, 26(1): 1-5.
[9]	WANG Ran, SHI Hai-dong, CHAI Ci-jiang, WEI Rao-pei, SONG Qiu-yue, SHI Yan-shan, LUO Jian-xia, ZHANG Yan. Studies on in vitro rooting and direct transplanting into ground of Cotoneaster hjelmqvistii plantlets in vitro in field environment [J]. Journal of Tianjin Agricultural University, 2018, 25(4): 1-5.
[10]	SONG Qiu-yue, WEI Rao-pei, CHAI Ci-jiang, WANG Ran, SHI Hai-dong, SHI Yan-shan, LUO Jian-xia. Effects of field and incubation chamber environment on in vitro rooting and survival of Cotoneaster hjelmqvistii micro shoots [J]. Journal of Tianjin Agricultural University, 2018, 25(2): 1-4.
[11]	SHI Yi, CHAI Ci-jiang, FENG Tao, HUANG Lei. Studies on gibberellin advancing dormant buds germinating and sterilizing and culturing technique of stem sections of myrobalan plums ( Prunus cerasifera Ehrh.) [J]. Journal of Tianjin Agricultural University, 2018, 25(2): 24-29.
[12]	YANG Guang-yan, WANG Kun-ying, CHAI Ci-jiang, LIU Hai-jing, GUAN Ming-yue. Effects of Environments on in Vitro Rooting and Survival of Malus zumi Micro Shoots [J]. Journal of Tianjin Agricultural University, 2017, 24(4): 15-18.
[13]	CHAI Ci-jiang, LU Xing-xia, QI Qian, SHA Lei. Effect of Soil Supporting Medium on Rooting of Stoneless Chinese Jujube Plantlet in Vitro [J]. Journal of Tianjin Agricultural University, 2017, 24(3): 28-31.
[14]	CHAI Ci-jiang, QI Zhi-bin, WANG Qiang-qiang, LU Xing-xia, SHI Yan-shan, LUO Jian-xia. Effect of Transplanting Period and Environmental Conditions on Survival and Growth of Cotoneaster hjelmqvistii Plantlet in Vitro [J]. Journal of Tianjin Agricultural University, 2017, 24(2): 22-24.
[15]	HAN Hui-hui, FU Ling-qiao, CHAI Ci-jiang, SU Wei-guo. Effect of Different Culture Conditions in Green-house on in Vitro Rooting and Survival of Ficus carica Micro-shoots [J]. Journal of Tianjin Agricultural University, 2016, 23(1): 23-27.