Journal of Tianjin Agricultural University ›› 2014, Vol. 21 ›› Issue (3): 12-16.

• Researches and Scientific Notes • Previous Articles     Next Articles

Isolation and Identification of Pathogen of Ascites Disease from Cynoglossus semilaevis Günther and Its Antibiotic Sensitivity Analysis

HU Xuan, SUN Jing-fengCorresponding Author, CHEN Cheng-xun, XING Ke-zhi   

  1. Tianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China
  • Received:2014-05-19 Online:2014-09-20 Published:2019-10-21

Abstract: Dominant bacteria were isolated from diseased Cynoglossus semilaevis Günther, and the artificial challenge assay showed that one of the strains (HST2) had strong pathogenicity to Cynoglossus semilaevis Günther. The tests on the morphological, cultural, physiological and biochemical characteristics, and the homology of 16S rRNA gene showed that HST2 matched to Vibrio parahaemolyticus. Drug resistance of HST2 to 50 antimicrobial agents was detected, and the results showed that HST2 was highly sensitive to 43 agents, such as aztreonam, sensitive to lincomycin, and resistant to 6 agents including penicillin G, ampicillin, carbenicillin, prostaphlin, bacitracin and azlocillin.

Key words: Cynoglossus semilaevis Günther;, ascites disease, Vibrio parahaemolyticus, isolation and identification, antibiotic sensitivity

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