玫瑰石斛叶片培养与原球茎诱导

doi:10.19640/j.cnki.jtau.2020.01.006

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (1): 24-27.doi: 10.19640/j.cnki.jtau.2020.01.006

• Researches and Scientific Notes • Previous Articles Next Articles

Leaf culture and protocorm induction of Dendrobium crepidatum

XU Ding-fan¹, LIU Yan-jun^{1,Corresponding Author}, HE Feng²

1. College of Horticulture and Landscape,Tianjin Agricultural University, Tianjin 300384, China;
2. Wei County Yiju Planting and Horticultural Design Co., Ltd., Handan 056800, Hebei Province, China

Received:2019-09-29 Published:2020-04-01

Abstract

Abstract: The leaves of Dendrobium crepidatum tissue culture seedlings were inoculated on MS medium containing different hormones to induce the production of protocorm, and then the protocorm was cultured for proliferation.The results showed that the protocorm induction effect was better on the medium with MS+2.0 mg/L 2,4-D+2.0 mg/L BA+0.5 mg/L NAA, and the induction rate could reach 94%; the protocorm could be induced with fast proliferation, hard texture and loose structure on MS+1.0 mg/L BA+0.2 mg/ L NAA. The results of this experiment can provide some reference for the study of artificial seeds of Dendrobium crepidatum.

Key words: Dendrobium crepidatum, leaf, protocorm, artificial seed

CLC Number:

S682.3

XU Ding-fan, LIU Yan-jun, HE Feng. Leaf culture and protocorm induction of Dendrobium crepidatum[J]. Journal of Tianjin Agricultural University, 2020, 27(1): 24-27.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2020.01.006

http://xuebao.tjau.edu.cn/EN/Y2020/V27/I1/24

References

[1] 李涛,何璇. 石斛属27种药用植物的性状鉴定特征比较[J].华西药学杂志,2016,31(1):54-57.
[2] 罗明. 三种水草枫斗类石斛特征图谱的研究及应用[D].广州:广州中医药大学,2014.
[3] 李付惠,丁长春,李蕾. 玫瑰石斛的生药学鉴定[J]. 文山学院学报,2011,24(3):16-17,40.
[4] 许丁帆,李建科,刘艳军,等. 铁皮石斛愈伤组织稳定培养体系的建立[J]. 天津农业科学,2018,24(8):67-69, 90.
[5] 罗明,魏刚,陈志辉,等. 玫瑰石斛HPLC特征图谱研究[J]. 中国现代中药,2014,16(4):269-273.
[6] 林妃,王必尊,常胜合,等. 影响香蕉人工种子再生因素研究[J]. 中国南方果树,2018,47(2):79-81.
[7] 郑惠元. 人参属代表植物人工种子包埋技术研究[D]. 北京:中国农业科学院,2018.
[8] 陈勋,王艳,刘畅宇,等. 白及种子繁育的研究现状[J].中国药房,2018,29(18):2585-2588.
[9] 周宝珍. 铁皮石斛人工种子的制作[J]. 陕西农业科学,2016,62(1):67-69.
[10] 段莹星. 植物组织培养技术的应用[J]. 农村经济与科技,2018,29(12):33.
[11] 李毅. 杂交构树体细胞胚诱导及人工种子的制备[D].大连:大连工业大学,2015.
[12] 苏江,陈林发,邓晰朝. 玫瑰石斛原球茎的诱导与增殖研究[J].种子,2016,35(3):85-88,92.
[13] 史滟滪,杨静慧,刘婷,等. 马铃薯松散型胚性愈伤组织的诱导[J]. 天津农业科学,2016,22(12):20-23.
[14] 李玉萍,王燕青,武文婷,等. 春兰与大花蕙兰杂交种原球茎分化和生根研究[J]. 天津农业科学,2015,21(8):127-132.
[15] 黄靖,刘春英,袁亚芳. 铁皮石斛原球茎诱导率提升的研究[J]. 福建农业科技,2018(11):4-6.
[16] 古碧珠,刘柏涛,何嘉碧,等. 兰花种子的原球茎诱导及其生长分化研究[J]. 安徽农业科学,2008(31):13511-13513.

Leaf culture and protocorm induction of Dendrobium crepidatum

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	An Ruiyu, Hao Zhibin. Regional segmentation method for wheat leaf diseases and insect pests based on semi-supervised active learning [J]. Journal of Tianjin Agricultural University, 2024, 31(2): 87-93.
[2]	Yang Cong, Zhou Qingyun, Liu Jia, Liao Jingwen, Li Ruoxin. Spatial variation characteristics of leaf light response during the grain filling period of summer maize in North China [J]. Journal of Tianjin Agricultural University, 2023, 30(6): 69-75.
[3]	Chai Xuxu, Bai Yanjiao, Weinila·Tuohetisun, Sun Xiaoling. Effects of leaf age and leaf position on leaf microstructure of Iris [J]. Journal of Tianjin Agricultural University, 2023, 30(5): 6-10.
[4]	Wen Hao, Ren Xiaoqin, Yang Jinghui, Li Bing, Zhang Chao, Wang Xing. Annual dynamic changes of leaf nutrient elements in high and low yield Dongzao orchards [J]. Journal of Tianjin Agricultural University, 2023, 30(4): 12-16.
[5]	Liu Wanqiu, Pang Junxiu, Cao Yannan, Wang Hui, Sun Yanjie, Sun Yafei, Long Hong. Leaf characteristics and drought resistance of triploid and diploid Salvia miltiorrhiza [J]. Journal of Tianjin Agricultural University, 2021, 28(1): 1-5.
[6]	Yin Sheng, Fu Yu, Long Hong. The vegetative phase change delay by leaf senescence related mutants ein3 and max2 in Arabidopsis thaliana [J]. Journal of Tianjin Agricultural University, 2020, 27(4): 7-11.
[7]	Zhang Jiaxue, Li Rui, Meng Jie, Yang Jinghui, Liu Yanjun, Wang Haixia. Comparison of growth characteristics of colored leaf plants on mild saline-alkali ground in Tianjin [J]. Journal of Tianjin Agricultural University, 2020, 27(4): 35-38.
[8]	ZHANG Wei-long, LI Diao, YANG Jing-hui, LI Rui, FENG Guo-hua, WANG Xing, ZHANG Chao. Infrared spectroscopy analysis of effects of different soil salinity on salt tolerance of 8 color-leaf trees [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 7-7.
[9]	XU Ding-fan, LIU Yan-jun, TAO Ze-man, XIAN Rui, FAN Li-na, YU Yi-han, XU Jiu, YANG Jing-hui. Study on embryogenic callus induction and proliferation of tomato [J]. Journal of Tianjin Agricultural University, 2020, 27(3): 22-25.
[10]	LU Jun, LI Rui, GAO Wei, YANG Jing-hui, LÜ Chen-fei, ZHANG Chao. Comparison of growth characteristics of colored leaf plants in different soils in Tianjin [J]. Journal of Tianjin Agricultural University, 2020, 27(2): 35-37.
[11]	QIAO Bo-chao, ZHOU Qiang, YANG Jing-hui, LI Diao, LI Bing, LIU Yan-jun. Physiological characteristics of different varieties of colored-leaf plants in moderate-severe saline-alkali soil [J]. Journal of Tianjin Agricultural University, 2019, 26(2): 31-34.
[12]	HUANG Yan, ZHOU Qiang, YANG Jing-hui, LI Zhen, LIU Yan-jun, WANG Xing, FENG Guo-hua. Effects of soil salinity on the growth of different species of colored leaf trees [J]. Journal of Tianjin Agricultural University, 2019, 26(2): 35-38.
[13]	GAO Zhong-hao, LI Zhen, YANG Jing-hui, ZHANG Chao, LIU Yan-jun, WANG Bin-bin. Anatomical structure of petiole and salt tolerant of 8 North American begonias [J]. Journal of Tianjin Agricultural University, 2019, 26(2): 39-42.
[14]	PANG Zhi-rui, ZHOU Qiang, YANG Jing-hui, LI Diao, GUI Yu, WANG Xing. Correlation of growth indicators on colored-leaf plants in different types of saline land [J]. Journal of Tianjin Agricultural University, 2018, 25(4): 10-12.
[15]	SONG Ke, ZHOU Qiang, YANG Jing-hui, MIAO Bo-ying, LI Diao, LIU Yan-jun. Differences of physiological characteristics among different species of color-leaf plants [J]. Journal of Tianjin Agricultural University, 2018, 25(3): 16-19.