拟南芥叶片衰老突变体ein3、max2延迟营养生长时相转变

doi:10.19640/j.cnki.jtau.2020.04.002

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (4): 7-11.doi: 10.19640/j.cnki.jtau.2020.04.002

• Researches and Scientific Notes • Previous Articles Next Articles

The vegetative phase change delay by leaf senescence related mutants ein3 and max2 in Arabidopsis thaliana

Yin Sheng, Fu Yu, Long Hong^{Corresponding Author}

College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-04-02 Online:2020-12-31 Published:2020-12-25

Abstract

Abstract: In higher plants, vegetative phase change refers to the transition from juvenile to adult phase, and after that plants can blossom. The vegetative phase change and the transition from vegetative growth to reproductive growth are important physiological processes in plant growth and development. Both processes are related to the timespan of plant development, sharing some regulated genes. However, regulation of leaf senescence related genes during this transition remains unknown. This study investigated the characteristics of vegetative phase change via leaf senescence related mutants ein3 and max2. Morphological observations of rosette leaf shape, onset of abaxial trichomes, blade base angles showed that vegetative phase change was delayed in ein3 and max2. Expression levels of miR156 and SPL3 at different stages of ein3 and max2 plant development showed down-regulated and up-regulated trend, leading to a delayed vegetative phase change. Compared with wild types, mutant plants developed slowly, due to the ongoing leaf senescence. The data suggested that EIN3 and MAX2 may play a role in regulating plant vegetative phase change through expression levels of miR156 and SPL3．

Key words: vegetative phase change, leaf senescence gene, ein3, max2, Arabidopsis thaliana

CLC Number:

Q945.4

Yin Sheng, Fu Yu, Long Hong. The vegetative phase change delay by leaf senescence related mutants ein3 and max2 in Arabidopsis thaliana[J]. Journal of Tianjin Agricultural University, 2020, 27(4): 7-11.

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://xuebao.tjau.edu.cn/EN/10.19640/j.cnki.jtau.2020.04.002

http://xuebao.tjau.edu.cn/EN/Y2020/V27/I4/7

References

[1] TELFER A,BOLLMAN K M,POETHIG R S.Phase change and the regulation of trichome distribution in Arabidopsis thaliana[J]. Development,1997,124(3):645-654.
[2] LI S,YANG X,WU F,et al.HYL1 controls the miR156- mediated juvenile phase of vegetative growth[J]. Journal of Experimental Botany,2012,63(7):2787-2798.
[3] WU G,POETHIG R S.Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3[J]. Development,2006,133(18):3539-3547.
[4] 徐倩,殷学仁,陈昆松. 基于乙烯受体下游转录因子的果实品质调控机制研究进展[J]. 园艺学报,2014,41(9):1913-1923.
[5] STIMBERG P,FURNER I J,LEYSER H M O. MAX2 participates in an SCF complex which acts locally at the node to suppress shoot branching[J]. Plant Journal,2007,50(1):80-94.
[6] WOO H R,CHUNG K M,Park J H,et al. ORE9,an F-box protein that regulates leaf senescence in Arabidopsis[J]. Plant Cell,2001,13(8):1779-1790.
[7] 卢阳,龙鸿. 拟南芥叶片数目变化突变体对营养生长时相转变的影响[J]. 植物学报,2015,50(3):331-337.
[8] CAO D,LI Y,WANG J,et al.GmmiR156b overexpression delays flowering time in soybean[J]. Plant Molecular Biology,2015,89:353-363.
[9] GUO C,XU Y,SHE M,et al.Repression of miR156 by miR159 regulates the timing of the juvenile-to-adult transition in Arabidopsis[J]. The Plant Cell,2017,29:1293-1304.
[10] FENG S,XU Y,GUO C.Modulation of miR156 to identify traits associated with vegetative phase change in tobacco(Nicotiana tabacum)[J]. Journal of Experimental Botany,2016,67(5):1493-1504.
[11] LEICHTYA A R,POETHIG R S.Development and evolution of age-dependent defenses in ant-acacias[J]. Proceedings of the National Academy of Sciences of the United States of America,2019,116(31):15596-15601.
[12] XU M,HU T,ZHAO J,et al.Developmental functions of miR156-regulated SQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL)genes in Arabidopsis thaliana[J]. PLOS Genetics,2016,12(8):e1006263.
[13] ZHANG H,ZHANG L,HAN J,et al.The nuclear localization signal is required for the function of squamosal promoter binding protein?like gene 9 to promote vegetative phase change in Arabidopsis[J]. Plant Molecular Biology,2019,100:571-578.
[14] GAN S,AMASINO R M.Making sense of senescence:molecular genetic regulation and manipulation of leaf senescence[J]. Plant Physiology,1997,113:313-319.
[15] XU Y,QIAN Z,ZHOU B,et al.Age-dependent heteroblastic development of leaf hairs in Arabidopsis[J]. New Phytologist,2019,224:741-748.
[16] SALEM M A,LI Y,BAJDZIENKO K,et al.RAPTOR controls developmental growth transitions by altering the hormonal and metabolic balance[J]. Plant Physiology,2018,177:565-593.
[17] FOURACREA J P,POETHIG R S.Role for the shoot apical meristem in the specification of juvenile leaf identity in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America,2019,116(20):10168-10177.
[18] HE J,XU M,WILLMANN M R,et al.Threshold- dependent repression of SPL gene expression by miR156/miR157 controls vegetative phase change in Arabidopsis thaliana[J]. PLOS Genetics,2018,14(4):e1007337.
[19] 郭蕊,傅钰,马婉琪,等. 拟南芥生物钟突变体gi延迟营养生长时相转变[J]. 生物学杂志,2018,35(3):15-18.
[20] XU Y,GUO C,ZHOU B,et al.Regulation of vegetative phase change by SWI2/SNF2 chromatin remodeling ATPase BRAHMA[J]. Plant Physiology,2016,172:2416-2428.
[21] XU M,HU T,SMITH M R,et al.Epigenetic regulation of vegetative phase change in Arabidopsis[J]. The Plant Cell,2016,28(1):28-41.
[22] XU Y,ZHANG L,WU G.Epigenetic regulation of juvenile-to-adult transition in plants[J]. Frontiers in Plant Science,2018,9:1048.

The vegetative phase change delay by leaf senescence related mutants ein3 and max2 in Arabidopsis thaliana

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments