萝卜叶黄酮的超声波辅助提取工艺及抗氧化活性研究

天津农学院学报 ›› 2017, Vol. 24 ›› Issue (2): 63-66.

萝卜叶黄酮的超声波辅助提取工艺及抗氧化活性研究

吴海清^1,2, 甄润英^{1,2,通信作者}, 何新益^1,2, 孙贵宝^1,2

1. 天津农学院食品科学与生物工程学院,天津 300384;
2. 天津市农副产品深加工技术工程中心,天津 300384

收稿日期:2016-07-10 出版日期:2017-06-30 发布日期:2019-10-15
通讯作者: 甄润英（1962–）,女,天津市人,教授,硕士,主要从事食品加工及营养检测方面的研究。E-mail：zhenrunying@tjau.edu.cn。
作者简介:吴海清（1982–）,女,天津市人,实验师,硕士,主要从事食品科学及生物工程方面的研究。E-mail：haiqing_w@yeah.net。
基金资助:
天津市农业科技合作项目“特色品牌青萝卜产业化配套新技术集成示范”（201410061027）

Optimization of Ultrasonic-assisted Extraction Technology and Antioxidant Activities of Flavonoids in Radish Leaves

WU Hai-qing^1,2, ZHEN Run-ying^{1,2,Corresponding Author}, HE Xin-yi^1,2, SUN Gui-bao^1,2

1. College of Food Science and Biotechnology, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Engineering and Technology Research Center of Agricultural Products Processing, Tianjin 300384, China

Received:2016-07-10 Online:2017-06-30 Published:2019-10-15

摘要/Abstract

摘要： 用超声波辅助乙醇提取萝卜叶中的黄酮。通过单因素试验与四元三次正交试验,对黄酮的超声波辅助醇提工艺进行优化,以体外清除（·OH）和（O₂^-·）的能力为指标,研究萝卜叶黄酮的抗氧化活性。最优条件：乙醇浓度为60%,料液比为1:15（m∶V）,超声波处理15 min,在50 ℃下浸提30 min,该条件下黄酮提取率为1.41%,是传统浸提法的200%;萝卜叶黄酮对·OH和O₂^-·均有明显的清除作用,在试验剂量下,清除效果随着黄酮浓度的增加而增强,对·OH和 O₂^-·的半数抑制率（IC₅₀）分别为0.643 8、1.523 1 mg/mL。结果表明：超声波辅助提取是一种高效的提取萝卜叶黄酮方法,其抗氧化活性较高。

关键词: 萝卜叶, 黄酮, 超声波, 辅助, 抗氧化

Abstract: Total flavonoids were extracted from radish leaves using ethanol as extraction solvent with the aid of ultrasonic treatment. Five single-factor experiments with four factors triple orthogonal design was adopted to research the ultrasonic-assisted ethanol extraction process of flavonoids in the water bath. The scavenging abilities against hydroxyl radicals （·OH） and （O₂^-·） in vitro of flavonoids were determined. The results indicated that the optimum extraction parameters were as follows：the volum fraction of ethanol was 60%, solid-liquid ratio was 15（m:V）, ultrasonic treatment 15min, immerse 30 min under the temperature of 50 ℃. Under these conditions, the extraction rate of total flavonoids is up to 1.41%, was the traditional extraction method of 200%. Flavonoids of radish leaves can effectively scavenge·OH and O₂^-·, and the role of these effect increased with the increase of flavonoids concentration, IC₅₀were 0.643 8, 1.523 1 mg/mL. These results demonstrated that UAE was a highly effective way to extract flavonoids from radish leaves and the antioxidant activity of flavonoids was high.

Key words: radish leaves, flavonoids, ultrasonic wave, assisted, antioxidant

中图分类号:

TS201.1

吴海清, 甄润英, 何新益, 孙贵宝. 萝卜叶黄酮的超声波辅助提取工艺及抗氧化活性研究[J]. 天津农学院学报, 2017, 24(2): 63-66.

WU Hai-qing, ZHEN Run-ying, HE Xin-yi, SUN Gui-bao. Optimization of Ultrasonic-assisted Extraction Technology and Antioxidant Activities of Flavonoids in Radish Leaves[J]. Journal of Tianjin Agricultural University, 2017, 24(2): 63-66.

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链接本文: http://xuebao.tjau.edu.cn/CN/

http://xuebao.tjau.edu.cn/CN/Y2017/V24/I2/63

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