水解猪肠黏膜及植物蛋白复合替代饲料鱼粉对大口黑鲈幼鱼生长性能、体组成和抗氧化能力的影响

doi:10.19640/j.cnki.jtau.2024.05.009

天津农学院学报 ›› 2024, Vol. 31 ›› Issue (5): 59-66.doi: 10.19640/j.cnki.jtau.2024.05.009

水解猪肠黏膜及植物蛋白复合替代饲料鱼粉对大口黑鲈幼鱼生长性能、体组成和抗氧化能力的影响

韩志豪^1a, 王振杰^1a, 龚埜^1a, 谢瑞涛², 陈乃松^1a,1b, 李松林^{1a,1b,通信作者}

1.上海海洋大学,a.农业农村部鱼类营养与环境生态研究中心;b.水产科学国家级实验教学示范中心,上海 201306;
2.广东恒兴饲料实业股份有限公司,广东湛江 524000

收稿日期:2023-10-11 出版日期:2024-10-31 发布日期:2024-11-05
通讯作者: 李松林（1989—）,男,副研究员,博士,主要从事肉食性鱼类营养与饲料方面的研究。E-mail：slli@shou.edu.cn。
作者简介:韩志豪（1998—）,男,硕士在读,研究方向为肉食性鱼类营养与饲料。E-mail：1337234866@qq.com。
基金资助:
国家现代农业产业技术体系项目（CARS-46）

Effects of the replacement dietary fishmeal with hydrolysed porcine mucosa and plant protein complexes on growth performance, body composition and antioxidant capacity of juvenile Largemouth bass（Micropterus salmoides）

Han Zhihao^1a, Wang Zhenjie^1a, Gong Ye^1a, Xie Ruitao², Chen Naisong^1a,1b, Li Songlin^{1a,1b,Corresponding Author}

1. Shanghai Ocean University, a. Centre for Research on Environmental Ecology and Fish Nutrition, Ministry of Agriculture and Rural Affairs, b. National Demonstration Center for Experimental Fisheries Science Education, Shanghai 201306, China;
2. Guangdong Evergreen Feed Industry Co., Ltd., Zhanjiang 524000, Guangdong Province, China

Received:2023-10-11 Online:2024-10-31 Published:2024-11-05

摘要/Abstract

摘要： 本实验旨在研究水解猪肠黏膜及植物蛋白复合替代鱼粉饲料对大口黑鲈（Micropterus salmoides）幼鱼生长性能、体组成和抗氧化能力的影响。配制4种等氮（51%粗蛋白）、等脂（12%粗脂肪）饲料,采用水解猪肠黏膜和植物蛋白（玉米蛋白和发酵豆粕）的复合混合物分级替代鱼粉。实验饲料中鱼粉含量分别为40%（FM40）、35%（FM35）、30%（FM30）和25%（FM25）,每组3个重复。以初始体重为（5.05±0.02）g的大口黑鲈为实验对象,进行为期70 d的养殖实验,每日表观饱食投喂2次。实验结果表明,在不影响生长性能的情况下,复合蛋白可以替代饲料中鱼粉用量的12.5%;同时,添加复合蛋白提高了饲料利用率（FER）及蛋白利用率（PER）,其中FM35组最高（P<0.05）;复合蛋白替代鱼粉对全鱼的水分、粗蛋白、粗脂肪及灰分含量没有显著影响（P>0.05）;复合蛋白替代鱼粉并未对血清中谷丙转氨酶（ALT）和谷草转氨酶（AST）活性产生显著影响（P>0.05）;在肝脏抗氧化能力方面,复合蛋白添加显著降低了MDA含量（P<0.05）,FM35组总抗氧化能力（T-AOC）与FM40组（对照组）相比没有显著差异（P>0.05）,但其他处理组显著低于FM40组（对照组）（P<0.05）;复合蛋白替代鱼粉对肝脏中过氧化氢酶（CAT）与超氧化物歧化酶（SOD）活力未产生显著性影响（P>0.05）,但FM35与对照组相比稍有提高（P>0.05）。综上所述,在不影响生长性能及肝脏健康指标的情况下,水解猪肠黏膜及植物蛋白复合替代鱼粉可将大口黑鲈幼鱼饲料中的鱼粉用量从40%降至35%。

关键词: 大口黑鲈, 水解猪肠黏膜, 鱼粉替代, 生长性能, 体成分, 抗氧化能力

Abstract: The aim of this experiment was to investigate the effects of a combination of hydrolysed porcine mucosa and plant proteins as an alternative protein source for fishmeal on growth performance, body composition and antioxidant capacity of juvenile Largemouth bass（Micropterus salmoides）. Four isonitrogenous（51% crude protein）and isolipid（12% crude lipid）diets were formulated with a graded replacement of fishmeal by a composite mixture of hydrolysed porcine mucosa and plant proteins（corn gluten meal and fermented soybean meal）. The fishmeal content of the test diets were 40%（FM40）, 35%（FM35）, 30%（FM30）and 25%（FM25）with three replicates per group. Largemouth bass with an initial body weight of（5.05±0.02）g were fed twice a day on an apparent satiation for 70 days. The results indicated that 12.5 % of dietary fishmeal could be replaced without affecting growth performance. Meanwhile, the addition of complex protein increased feed efficiency rate（FER）and protein efficiency rate（PER）, which was significantly increased in the FM35 group（P<0.05）. Substitution of fishmeal with complex protein did not have significant effects on moisture, protein, lipid and ash of whole fish（P>0.05）. Additionally, the replacement of fishmeal did not have significant effects on serum alanine transaminase（ALT）and aspartate transaminase （AST）activities（P>0.05）. In terms of hepatic antioxidant capacity, the addition of complex proteins significantly reduced the malondialdehyde（MDA）content. The total antioxidant capacity（T-AOC）of the FM35 group did not produce a significant difference from that of the FM40（control）（P>0.05）, but the other treatment groups were significantly lower than that of the FM40（control）（P<0.05）. The hepatic catalase（CAT）and superoxide dismutase（SOD）in the liver were not significantly affected by the complex proteins substitution, but FM35 showed a slight increase compared to FM40（control）（P>0.05）. It is concluded that replacement of fishmeal by a combination of hydrolysed porcine mucosa and plant proteins could reduce the fishmeal content of juvenile Largemouth bass diets from 40% to 35% without affecting growth performance and liver health indicators.

Key words: Largemouth bass, hydrolysed porcine mucosa, fishmeal replacement, growth performance, body composition, antioxidant capacity

中图分类号:

S963.1

韩志豪, 王振杰, 龚埜, 谢瑞涛, 陈乃松, 李松林. 水解猪肠黏膜及植物蛋白复合替代饲料鱼粉对大口黑鲈幼鱼生长性能、体组成和抗氧化能力的影响[J]. 天津农学院学报, 2024, 31(5): 59-66.

Han Zhihao, Wang Zhenjie, Gong Ye, Xie Ruitao, Chen Naisong, Li Songlin. Effects of the replacement dietary fishmeal with hydrolysed porcine mucosa and plant protein complexes on growth performance, body composition and antioxidant capacity of juvenile Largemouth bass（Micropterus salmoides）[J]. Journal of Tianjin Agricultural University, 2024, 31(5): 59-66.

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http://xuebao.tjau.edu.cn/CN/Y2024/V31/I5/59

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水解猪肠黏膜及植物蛋白复合替代饲料鱼粉对大口黑鲈幼鱼生长性能、体组成和抗氧化能力的影响

Effects of the replacement dietary fishmeal with hydrolysed porcine mucosa and plant protein complexes on growth performance, body composition and antioxidant capacity of juvenile Largemouth bass（Micropterus salmoides）

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