锦鲤N-乙酰氨基葡萄糖-1-磷酸转移酶α/β亚基（GNPTAB）的生物信息学分析及其组织分布和时序表达

doi:10.19640/j.cnki.jtau.2024.05.008

天津农学院学报 ›› 2024, Vol. 31 ›› Issue (5): 52-58.doi: 10.19640/j.cnki.jtau.2024.05.008

锦鲤N-乙酰氨基葡萄糖-1-磷酸转移酶α/β亚基（GNPTAB）的生物信息学分析及其组织分布和时序表达

温思怡, 韩卓然, 孙敬锋^通信作者

天津农学院水产学院,天津 300392

收稿日期:2024-02-26 出版日期:2024-10-31 发布日期:2024-11-05
通讯作者: 孙敬锋（1976—）,男,教授,博士,主要从事水产动物免疫与疾病防控研究。E-mail：sun_jf@163.com。
作者简介:温思怡（1999—）,女,硕士在读,主要从事水产动物免疫与疾病防控研究。E-mail：wenyian0102@163.com。
基金资助:
天津市海水养殖产业技术体系创新团队项目（ITTMRS2021007）; 天津市自然科学基金项目（23JCQNJC00490）

Bioinformatics analysis, tissue distribution and temporal expression of alpha/beta subunits of N-acetylglucosamine-1-phosphotransferase from Cyprinus carpio var. koi

Wen Siyi, Han Zhuoran, Sun Jingfeng^{Corresponding Author}

College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2024-02-26 Online:2024-10-31 Published:2024-11-05

摘要/Abstract

摘要： 论文旨在研究锦鲤（Cyprinus carpio var. koi）GNPTAB（alpha/beta subunits of N-acetylglucosamine- 1-phosphotransferase）基因的分子生物学特征及对维氏气单胞菌感染的应答规律。对锦鲤GNPTAB基因CDS区进行生物信息学分析,利用实时荧光定量PCR技术研究GNPTAB基因的组织分布和维氏气单胞菌（Aeromonas veronii）感染机体后的时序表达规律。结果显示,GNPTAB基因CDS区片段长度为3 747 bp,推测编码1 248个氨基酸,含有4个结构域。实时荧光定量PCR分析显示,GNPTAB基因在健康锦鲤各组织中均有表达,在肝脏表达量最高,其次是肌肉、肠道、脾脏、皮肤、中肾、头肾。维氏气单胞菌人工感染锦鲤6~72 h,GNPTAB基因在肠道组织主要呈现上调表达,在肝脏、头肾、脾脏、皮肤、肌肉组织中主要呈现下调表达。维氏气单胞菌感染锦鲤后,GNPTAB基因在这些组织中出现表达差异,推测GNPTAB参与了机体的病理生理过程或免疫应答反应。

关键词: 锦鲤, 生物信息学分析, 组织分布, 时序表达, N-乙酰氨基葡萄糖-1-磷酸转移酶α/β亚基

Abstract: This study aimed at exploring the molecular characteristics of GNPTAB（alpha / beta subunits of N-acetylglucosamine- 1-phosphotransferase）gene and its immune response to infection with Aeromonas veronii in koi carp Cyprinus carpio var. koi. The CDS region of GNPTAB gene in koi carp was analyzed by bioinformatics. Real-time fluorescence quantitative PCR technology was applied to investigate the tissue distribution of GNPTAB gene from healthy C. carpio var. koi and its temporal expression in different tissues of the fish following challenging with A.veronii. The results showed that the length of GNPTAB gene CDS fragment was 3 747 bp, which encoded 1 248 amino acid and contained four specific domains. Real-time fluorescence Quantitative PCR analysis showed that GNPTAB was expressed in all tested tissues of healthy C. carpio var. koi, with the highest expression in the liver, followed by the muscle, intestine, spleen, skin, midkidney and head kidney. During 6-72 h after experimental infection with A. veronii in the C. carpio var. koi, the expression of GNPTAB was up-regulated in the intestine, while down-regulated in the liver, head kidney, spleen, skin and muscle. The differentiated expression in the tested tissues following infection with A. veronii in the C. carpio var. koi suggested that GNPTAB was involved in the pathophysiological process or the immune response against A. veronii.

Key words: Cyprinus carpio var. koi, bioinformatics analysis, tissue distribution, temporal expression, GNPTAB

中图分类号:

S917

温思怡, 韩卓然, 孙敬锋. 锦鲤N-乙酰氨基葡萄糖-1-磷酸转移酶α/β亚基（GNPTAB）的生物信息学分析及其组织分布和时序表达[J]. 天津农学院学报, 2024, 31(5): 52-58.

Wen Siyi, Han Zhuoran, Sun Jingfeng. Bioinformatics analysis, tissue distribution and temporal expression of alpha/beta subunits of N-acetylglucosamine-1-phosphotransferase from Cyprinus carpio var. koi[J]. Journal of Tianjin Agricultural University, 2024, 31(5): 52-58.

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http://xuebao.tjau.edu.cn/CN/Y2024/V31/I5/52

参考文献

[1] CATHEY S S,KUDO M,TIEDE S,et al.Molecular order in mucolipidosis II and III nomenclature[J]. American Journal of Medical Genetics,2008,146(4):512.
[2] LEI Y,IWASHITA M,CHOI J,et al.N- acetylglucosamine-1-phosphate transferase suppresses lysosomal hydrolases in dysfunctional osteoclasts:a potential mechanism for vascular calcification[J]. Journal of Cardiovascular Development and Disease,2015,2(2):31-47.
[3] BRAULKE T,POHL S,STORCH S.Molecular analysis of the glcnac-1-phosphotransferase[J]. Journal of Inherited Metabolic Disease,2008,31(2):253-257.
[4] MAO S J,ZU Y M,DAI Y L et al. Gnptabcase report:mucolipidosis II and III alpha/beta caused by pathogenic variants in the gene(mucolipidosis)[J]. Frontiers in Pediatrics,2022,10:852701.
[5] KOLLMANN K,PESTKA J M,KÜHN S C,et al. Decreased bone formation and increased osteoclast genesis cause bone loss in mucolipidosis II[J]. EMBO Molecular Medicine,2013,5:1871-1886.
[6] KANG C,DRAYNA D.A role for inherited metabolic deficits in persistent developmental stuttering[J]. Molecular Genetics and Metabolism,2012,107:276-280.
[7] GELFMAN C M,VOGEL P,ISSA T M,et al.Mice lacking alpha/beta subunits of glcnac-1- phosphotransferase exhibit growth retardation,retinal degeneration,and secretory cell lesions[J]. Investigative Ophthalmology and Visual Science,2007,48(11):5221-5228.
[8] 王磊,张炳华,杨文涛,等. SARS-CoV-2感染与非感染人群血清1-10 ku蛋白质组差异性分析[J]. 中国病原生物学杂志,2021,16(10):1115-1121,1128.
[9] WANG Y,XIU Y,BI K,et al.Integrated analysis of mRNA-seq in the haemocytes of Eriocheir sinensis in response to Spiroplasma eriocheiris infection[J]. Fish & Shellfish Immunology,2017,68:289-298.
[10] 陈湘粦,乐佩琦,林人端. 鲤科的科下类群及其宗系发生关系[J]. 动物分类学报,1984(4):424-440.
[11] NEDOLUZHKO A V,GLADYSHEVA-AZGARI M V,SHALGIMBAYEVA G M,et al. Genetic contribution of domestic European common carp(Cyprinus carpio carpio)and Amur carp(Cyprinus carpio haematopterus)to the wild Vietnamese carp population as revealed by ddRAD sequencing[J]. Aquaculture,2021,544:737049.
[12] 林伟,伦峰,杨治国. 锦鲤体表溃烂病病原的分离鉴定[J]. 黑龙江畜牧兽医,2016(11):206-208.
[13] 杨昆明,党瑞,谢志胜,等. 锦鲤致病性维氏气单胞菌的分离、鉴定及药敏研究[J]. 淡水渔业,2017,47(5):47-52.
[14] CHEN F,SUN J F,HAN Z R,et al.Isolation,identification and characteristics of Aeromonas veronii from diseased crucian carp(Carassius auratus gibelio)[J]. Frontiers in Microbiology,2019,10:2742.
[15] ABDEL R,ELSHESHTAWY H,YASSIN E,et al.Hematological,immuno-antioxidant disruptions,and genes down-regulation induced by Aeromonas veronii challenge in Clarias gariepinus:The ameliorative role of silica nanoparticles[J]. Fish & Shellfish Immunology,2023,138:108842.
[16] HU X,XIAO Z,LI B,et al.Isolation,identification,and characterization of Aeromonas veronii from Chinese Soft-Shelled Turtle(Trionyx sinensis)[J]. Microorganisms,2023,11(5):1304.
[17] HAN Z R,SUN J F,JIANG B Y,et al.Concurrent infections of Aeromonas veronii and Vibrio cholerae in koi carp (Cyprinus carpio var. koi)[J]. Aquaculture,2021,535:736395.
[18] CATHEY S S,LEROY J G,WOOD T,et al.Phenotype and genotype in mucolipidoses II and III alpha/beta:a study of 61 probands[J]. Journal of Medical Genetics,2010,47(1):38-48.
[19] LEROY J G,SILLENCE D,WOOD T,et al.A novel intermediate mucolipidosis II/IIIαβ caused by GNPTAB mutation in the cytosolic n-terminal domain[J]. European Journal of Human Genetics,2014,22(5):594-601.
[20] ZHANG Z,YUE P,LU T,et al.Role of lysosomes in physiological activities,diseases,and therapy[J]. Journal of Hematology and Oncology,2021,14(1):79.
[21] BRUSLÉ J,ANADON G G.The structure and function of fish liver[M]. London:Routledge,2017.
[22] DAWOOD M A O. Nutritional immunity of fish intestines:important insights for sustainable aquaculture[J]. Reviews in Aquaculture,2021,13(1):642-663.
[23] YI G,LI L,LUO M,et al.Heat stress induces intestinal injury through lysosome-and mitochondria-dependent pathway in vivo and in vitro[J]. Oncotarget,2017,8(25):40741.
[24] ZHANG N,ZHANG H,DONG Z,et al.Molecular identification of Nocardia seriolae and comparative analysis of spleen transcriptomes of hybrid snakehead(Channa maculata female×Channa argus male)with nocardiosis disease[J]. Frontiers in Immunology,2022,13:778915.
[25] KIM J H,CHOI T G,PARK S,et al.Mitochondrial ROS-derived PTEN oxidation activates PI3K pathway for mTOR-induced myogenic autophagy[J]. Cell Death & Differentiation,2018,25(11):1921-1937.
[26] MIAO G,ZHAO H,LI Y,et al.ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation[J]. Developmental Cell,2021,56(4):427-442.
[27] XU Z,PARRA D,GÓMEZ D,et al. Teleost skin,an ancient mucosal surface that elicits gut-like immune responses[J]. Proceedings of the National Academy of Sciences,2013,110(32):13097-13102.
[28] ELLIS A E.Immunity to bacteria in fish[J]. Fish & Shellfish Immunology,1999,9(4):291-308.
[29] SAURABH S,SAHOO P K.Lysozyme:an important defence molecule of fish innate immune system[J]. Aquaculture Research,2008,39(3):223-239.
[30] ARMA N R,HIRONO I,AOLI T.Genes expressed in Japanese flounder Paralichthys olivaceus spleen:analysis of genes involved in immune function[J]. Fisheries Science,2005,71(6):1304-1323.
[31] CAI Y,WANG S,GUO W,et al.Transcriptome analysis provides insights into the immune responsive pathways and genes in the head kidney of tiger grouper(Epinephelus fuscoguttatus)fed with Spatholobus suberectus,Phellodendron amurense,or Eclipta prostrata[J]. Fish & Shellfish Immunology,2018,73:100-111.

锦鲤N-乙酰氨基葡萄糖-1-磷酸转移酶α/β亚基（GNPTAB）的生物信息学分析及其组织分布和时序表达

Bioinformatics analysis, tissue distribution and temporal expression of alpha/beta subunits of N-acetylglucosamine-1-phosphotransferase from Cyprinus carpio var. koi

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