点带石斑鱼致病性哈维氏弧菌的分离鉴定及药敏试验

doi:10.19640/j.cnki.jtau.2021.04.009

Journal of Tianjin Agricultural University ›› 2021, Vol. 28 ›› Issue (4): 35-40.doi: 10.19640/j.cnki.jtau.2021.04.009

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation, identification and antibiotic susceptibility analysis of the pathogenic Vibrio harveyi from Epinephelus coioides

Pei Lishuo, Zhu Yonglong, Mao Qing, Chen Limei, Guo Yongjun, Sun Jingfeng^{Corresponding Author}

Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-03-28 Online:2021-12-31 Published:2022-01-05

Abstract

Abstract: To explore the cause of death of Epinephelus coioides, a bacterial strain was isolated from the liver of diseased Epinephelus coioides and named as SBY-G. This bacterial strain was preliminarily determined as Vibrio harveyi according to its morphological, physiological and biochemical characteristics. The sequence analysis of the 16S rDNA and gapA genes respectively showed SBY-G had the highest similarity with V. harveyi（KU364055.1）and（LC370081.1）, and the phylogenic trees showed that it was clustered with V. harveyi. The results of antibiotic susceptibility analysis showed that SBY-G was highly sensitive to amoxicillin, cefixime, cefamandole, ceftriaxone, norfloxacin and other fifteen antibiotics, moderate sensitive to cefotaxime, gentamicin, kanamycin and other six antibiotics, while resistant to ampicillin, oxacillin, cefuroxime, ceftazidime, streptomycin, neomycin and other eighteen antibiotics. Artificial infection experiments showed that the median lethal dosage（LD₅₀）of the SBY-G strain on zebra fish was calculated as 1×10⁵cfu/g. The results of this study provide a scientific reference for the prevention, treatment and diagnosis of fish diseases caused by V. harveyi.

Key words: Vibrio harveyi, 16S rDNA, gapA, antibiotic susceptibility analysis

CLC Number:

Q959.48

Pei Lishuo, Zhu Yonglong, Mao Qing, Chen Limei, Guo Yongjun, Sun Jingfeng. Isolation, identification and antibiotic susceptibility analysis of the pathogenic Vibrio harveyi from Epinephelus coioides[J]. Journal of Tianjin Agricultural University, 2021, 28(4): 35-40.

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Isolation, identification and antibiotic susceptibility analysis of the pathogenic Vibrio harveyi from Epinephelus coioides

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