萝卜叶黄酮的超声波辅助提取工艺及抗氧化活性研究

Journal of Tianjin Agricultural University ›› 2017, Vol. 24 ›› Issue (2): 63-66.

• Researches and Scientific Notes • Previous Articles Next Articles

Optimization of Ultrasonic-assisted Extraction Technology and Antioxidant Activities of Flavonoids in Radish Leaves

WU Hai-qing^1,2, ZHEN Run-ying^{1,2,Corresponding Author}, HE Xin-yi^1,2, SUN Gui-bao^1,2

1. College of Food Science and Biotechnology, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Engineering and Technology Research Center of Agricultural Products Processing, Tianjin 300384, China

Received:2016-07-10 Online:2017-06-30 Published:2019-10-15

Abstract

Abstract: Total flavonoids were extracted from radish leaves using ethanol as extraction solvent with the aid of ultrasonic treatment. Five single-factor experiments with four factors triple orthogonal design was adopted to research the ultrasonic-assisted ethanol extraction process of flavonoids in the water bath. The scavenging abilities against hydroxyl radicals （·OH） and （O₂^-·） in vitro of flavonoids were determined. The results indicated that the optimum extraction parameters were as follows：the volum fraction of ethanol was 60%, solid-liquid ratio was 15（m:V）, ultrasonic treatment 15min, immerse 30 min under the temperature of 50 ℃. Under these conditions, the extraction rate of total flavonoids is up to 1.41%, was the traditional extraction method of 200%. Flavonoids of radish leaves can effectively scavenge·OH and O₂^-·, and the role of these effect increased with the increase of flavonoids concentration, IC₅₀were 0.643 8, 1.523 1 mg/mL. These results demonstrated that UAE was a highly effective way to extract flavonoids from radish leaves and the antioxidant activity of flavonoids was high.

Key words: radish leaves, flavonoids, ultrasonic wave, assisted, antioxidant

CLC Number:

TS201.1

WU Hai-qing, ZHEN Run-ying, HE Xin-yi, SUN Gui-bao. Optimization of Ultrasonic-assisted Extraction Technology and Antioxidant Activities of Flavonoids in Radish Leaves[J]. Journal of Tianjin Agricultural University, 2017, 24(2): 63-66.

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Optimization of Ultrasonic-assisted Extraction Technology and Antioxidant Activities of Flavonoids in Radish Leaves

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