小鼠骨骼肌卫星细胞的分离培养和鉴定

Journal of Tianjin Agricultural University ›› 2014, Vol. 21 ›› Issue (1): 1-4.

• Researches and Scientific Notes • Next Articles

Isolated Culture and Identification of Mouse Skeletal Muscle Satellite Cells

DAI Yang¹, WANG Yi-min^1,2, LIU Xin-feng¹, FAN Han-lu¹, LI Ji-xia¹, GUO Hong¹, DING Xiang-bin^{1,Corresponding Author}

1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;
2. Institute of Bioengineering, Northwest A&F University, Yangling 712100, Shaanxi Province, China

Received:2013-12-19 Published:2019-10-21

Abstract

Abstract: In this study, mouse skeletal muscle satellite cells were isolated by collagenase and trypsinase digestion method. Then the satellite cells were purified and induced to differentiate into myotubes, and the marker genes of the cells before or after the differentiation were detected by RT-PCR and immunocytochemistry technology. The results show that the isolated muscle satellite cells were healthy, and expressed the marker genes of paired protein box (Pax7) and myogenic differentiation antigen (MyoD). After inducing to the myotubes, myogenin (MyoG) and myosin heavy chain (MHC)showed positive expression. The results indicate that mouse skeletal muscle satellite cells of high purity, which can provide cell sources for muscle development and damage repair study, are successfully isolated.

Key words: mouse, skeletal muscle satellite cell, cell culture, identification

CLC Number:

Q813.1

DAI Yang, WANG Yi-min, LIU Xin-feng, FAN Han-lu, LI Ji-xia, GUO Hong, DING Xiang-bin. Isolated Culture and Identification of Mouse Skeletal Muscle Satellite Cells[J]. Journal of Tianjin Agricultural University, 2014, 21(1): 1-4.

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Isolated Culture and Identification of Mouse Skeletal Muscle Satellite Cells

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