探索DNA条形码对哈摇蚊类群部分属物种鉴定的有效性

doi:10.19640/j.cnki.jtau.2020.03.013

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (3): 57-61.doi: 10.19640/j.cnki.jtau.2020.03.013

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Exploring the utility of DNA barcoding in species identification of Harischia complex

YU Hai-jun, HUANG Xin, ZHANG Yu-ying, ZHOU Jing-jing, TIAN Zhao-zhi, CHEN Jia-lin, WANG Qian^{Corresponding Author}

Key Laboratory of Aquatic-Ecology and Aquaculture of Tianjin, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-06-26 Online:2020-10-10 Published:2020-10-10

Abstract

Abstract: This study collected Harischia complex species in regions of China, using DNA barcoding for species identification. The results showed: the mean interspecific genetic distance was 2.8 times larger than the mean intraspecific genetic distance, with existing distinct “DNA barcoding gap”. Based on Automatic Barcode Gap Discovery genetic distance was analyzed, and it was related with distributed frequency. The results of Neighbor joining tree analysis pointed that 87.5% of the species were distinguished, and it kept conformed to morphological identification. Our results produced basal data for exploring the utility of DNA barcoding in species identification of Harischia complex, and provided abundant material for filling up the Chinese DNA barcodes database of Chironomidae.

Key words: Harischia complex, DNA barcoding, species identification, genetic distance

CLC Number:

S931.1

YU Hai-jun, HUANG Xin, ZHANG Yu-ying, ZHOU Jing-jing, TIAN Zhao-zhi, CHEN Jia-lin, WANG Qian. Exploring the utility of DNA barcoding in species identification of Harischia complex[J]. Journal of Tianjin Agricultural University, 2020, 27(3): 57-61.

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Exploring the utility of DNA barcoding in species identification of Harischia complex

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