半滑舌鳎黏蛋白MUC5AC基因的克隆与结构预测

doi:10.19640/j.cnki.jtau.2019.02.012

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (2): 51-56.doi: 10.19640/j.cnki.jtau.2019.02.012

• Researches and Scientific Notes • Previous Articles Next Articles

Cloning and sequence analysis of mucin MUC5AC gene from the half-smooth tongue sole, Cynoglossus semilaevis

TAN Jing, HU Xiu-cai, LÜ Ai-jun, SUN Jing-feng, LIU Xiao-xue, LIAN Zheng-yi

Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-01-27 Online:2019-06-20 Published:2019-09-19

Abstract

Abstract: As the main component of fish mucus, mucin plays an important role in protecting from pathogen infection. The MUC5AC gene of Cynoglossus semilaevis, namely CsMUC5AC gene was cloned by RT-PCR method and protein structure was predicted by bioinformatics analysis. Results showed that the length of the CsMUC5AC gene fragment was 2 771 bp, encoding 917 amino acids（aa）, and 5 antigenic epitopes were predicted to be mainly located within 314-321, 507-516, 578-589, 748-762 and 811-826 aa sites. The 3 domains including C8（164-238, 624-698 aa）, VWD（1-128, 430-588 aa）and VWC（303-365, 768-836 aa）were observed. Analysis of secondary structure showed that the C8 motif was α-helix, the VWD domain was β-sheet, and the tertiary structure was two subunits profiles（VWD-C8-VWC）. The phylogenetic tree analysis showed that CsMUC5AC was closely related to that of Scophthalmus maximus, Anabas testudineus and Oreochromis niloticus, and naturally clustered into one group with the secretory mucins. These results provide a scientific reference for the further study of MUC5AC gene function in tongue sole.

Key words: Cynoglossus semilaevis, MUC5AC, gene cloning, structure prediction

CLC Number:

S917.4

TAN Jing, HU Xiu-cai, LÜ Ai-jun, SUN Jing-feng, LIU Xiao-xue, LIAN Zheng-yi. Cloning and sequence analysis of mucin MUC5AC gene from the half-smooth tongue sole, Cynoglossus semilaevis[J]. Journal of Tianjin Agricultural University, 2019, 26(2): 51-56.

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References

[1] Lee H S,Lee H K,Kim H S,et al.MUC1,MUC2,MUC5AC,and MUC6 expressions in gastric carcinomas[J]. Cancer,2015,92(6):1427-1434.
[2] Bonser L R,Zlock L,Finkbeiner W,et al.Epithelial tethering of MUC5AC-rich mucus impairs mucociliary transport in asthma[J]. Journal of Clinical Investigation,2016,126(6):2367-2371.
[3] Betge J,Schneider N I,Harbaum L,et al.MUC1,MUC2,MUC5AC,and MUC6 in colorectal cancer:expression profiles and clinical significance[J]. Virchows Archiv,2016,469(3):255-265.
[4] 吕爱军,胡秀彩,孙敬锋,等. 鱼类皮肤免疫应答及蛋白质组学[J]. 水产科学,2016,35(3):302-307.
[5] 杨桂文,安利国. 鱼类黏液细胞研究进展[J]. 水产学报,1999(4):403-408.
[6] Welsh K G,Rousseau K,Fisher G,et al.MUC5AC and a glycosylated variant of MUC5B alter mucin composition in children with acute asthma[J]. Chest,2017,152(4):771-779.
[7] McGuckin M A,Lindén S K,Sutton P,et al. Mucin dynamics and enteric pathogens[J]. Nature Reviews Microbiology,2011,9(4):265-278.
[8] Hovenberg H W,Davies J R,Carlstedt I.Different mucins are produced by the surface epithelium and the submucosa in human trachea:identification of MUC5AC as a major mucin from the goblet cells[J]. Biochemical Journal,1996,318(1):319-324.
[9] Allen A,Hutton D A,Pearson J P,et al.Mucus glycoprotein structure,gel formation and gastrointestinal mucus function[J]. Ciba Foundation Symposium,1984,109(12):137.
[10] Lang T,Klasson S,Larsson E,et al.Searching the evolutionary origin of epithelial mucus protein components- mucins and FCGBP[J]. Molecular Biology & Evolution,2016,33(8):1921-1936.
[11] 薛春雨,董晶晶,习丙文,等. 团头鲂黏蛋白基因Muc5b克隆及表达分析[J]. 动物学杂志,2014,49(6):886-896.
[12] Cao X J, Wang W M.Histology and mucin histochemistry of the digestive tract of yellow catfish,Pelteobagrus fulvidraco[J]. Anatomia Histologia Embryologia,2010,38(4):254-261.
[13] Han Z R,Sun J F,Lü A J,et al.Isolation,identification and characterization of Shewanella algae from reared tongue sole,Cynoglossus semilaevis Günther[J]. Aquaculture,2017,468:356-362.
[14] Sveen L R,Grammes F T,Ytteborg E,et al.Genome-wide analysis of Atlantic salmon (Salmo salar) mucin genes and their role as biomarkers[J]. Plos One,2017,12(12):e0189103.
[15] Marel M V D,Adamek M,Gonzalez S F,et al. Molecular
cloning and expression of two β-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after β-glucan feeding[J]. Fish & Shellfish Immunology,2012,32(3):494-501.
[16] Neuwirth L S,Phillips G R,Abdeslem E I .Perinatal Pb²+exposure alters the expression of genes related to the neurodevelopmental GABA-shift in postnatal rats[J]. Journal of Biomedical Science,2018,25(1):45.
[17] 梁昱,刘燕霏,孙晓莉,等. 犬链球菌M蛋白基因的克隆及生物信息学分析[J]. 天津农学院学报,2018, 25(1):44-48.
[18] Benhamed S,Guardiola F A,Mars M,et al.Pathogen bacteria adhesion to skin mucus of fishes[J]. Veterinary Microbiology,2014,171(1-2):1-12.
[19] Hjelmeland K,Christie M,Raa J.Skin mucus protease from rainbow trout,Salmo gairdneri Richardson,and its biological significance[J]. Journal of Fish Biology,1983,23(1):10.
[20] Pérez-Sánchez J,Itziar E,Redondo M J,et al.Mucins as diagnostic and prognostic biomarkers in a fish-parasite model:transcriptional and functional analysis[J]. Plos One,2013,8(6):e65457.
[21] Mejías-Luque R,Lindén S K,Garrido M,et al.Inflammation modulates the expression of the intestinal mucins MUC2 and MUC4 in gastric tumors[J]. Oncogene,2010,29(12):1753-1762.
[22] Matsukita S,Nomoto M,Kitajima S,et al.Expression of mucins(MUC1,MUC2,MUC5AC and MUC6)in mucinous carcinoma of the breast:comparison with invasive ductal carcinoma[J]. Histopathology,2010,42(1):26-36.
[23] Imai Y,Yamagishi H,Fukuda K,et al.Differential mucin phenotypes and their significance in a variation of colorectal carcinoma[J]. World J Gastroenterol,2013,19(25):3957-3968.
[24] Duan Y,Liu Q,Wang Y,et al.Impairment of the intestine barrier function in,Litopenaeus vannamei,exposed to ammonia and nitrite stress[J]. Fish & Shellfish Immunology,2018,78:279-288.

Cloning and sequence analysis of mucin MUC5AC gene from the half-smooth tongue sole, Cynoglossus semilaevis

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