虾肝肠胞虫环介导等温扩增法（LAMP）的建立

doi:10.19640/j.cnki.jtau.2018.02.013

Journal of Tianjin Agricultural University ›› 2018, Vol. 25 ›› Issue (2): 51-54.doi: 10.19640/j.cnki.jtau.2018.02.013

• Researches and Scientific Notes • Previous Articles Next Articles

Establishment of a LAMP assay for detection of Enterocytozoon hepatopenaei

SUN Yan, DONG Xue-wang, WEI Jun-li, XUE Shu-xia^{Corresponding Author}

Tianjin Diseases Prevention and Control Center of Aquatic Animals, Tianjin 300221, China

Received:2018-01-31 Online:2018-06-20 Published:2019-11-12

Abstract

Abstract: In order to develop a rapid method for detecting the Enterocytozoon hepatopenaei (EHP), a loop-mediated isothermal amplification (LAMP) assay was established for targeting EHP-SSU gene with six specific primers following the optimization of the reaction. The results showed that the lowest limit detection of EHP-LAMP assay was 3.71 copies/μL, then its specificity was verified, and no cross-reactions was found for other shrimp pathogenic viruses including WSSV, IHHNV and CMNV. Moreover, 30 samples of shrimp with typical EHP infection symptoms were detected with the LAMP and PCR method, respectively. The results showed that the positive rates of LAMP and PCR were both 100%. In conclusion, the EHP-LAMP method is suitable for EHP early diagnosis with the advantages of high sensitivity, specificity and accuracy.

Key words: shrimp, Enterocytozoon hepatopenaei (EHP), loop-mediated isothermal amplification (LAMP), detection

CLC Number:

Q786
S917.4

SUN Yan, DONG Xue-wang, WEI Jun-li, XUE Shu-xia. Establishment of a LAMP assay for detection of Enterocytozoon hepatopenaei[J]. Journal of Tianjin Agricultural University, 2018, 25(2): 51-54.

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Establishment of a LAMP assay for detection of Enterocytozoon hepatopenaei

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