番茄愈伤组织筛选及抗逆突变体的获得

doi:10.19640/j.cnki.jtau.2022.03.002

Journal of Tianjin Agricultural University ›› 2022, Vol. 29 ›› Issue (3): 9-13.doi: 10.19640/j.cnki.jtau.2022.03.002

• Researches and Scientific Notes • Previous Articles Next Articles

Screening of tomato callus and acquisition of stress resistant mutants

He Yuanqin¹, Xu Dingfan¹, Liu Yanjun^{1,Corresponding Author}, Ji Heliang², Zhao Lili¹, Dong Hongyu¹

1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Gengyun Seed Co. Ltd., Tianjin 300408, China

Received:2021-11-08 Online:2022-09-30 Published:2022-10-10

Abstract

Abstract: Tomato petioles were used as explants to establish tomato callus regeneration system, then the mutant cells of tomato callus were screened by PEG-8000, and the regeneration of callus cells was induced at the same time, so as to screen regenerated plants with strong growth potential and stress resistance. The results showed that the callus with hard texture and loose structure could be induced on the medium of MS + 1.0 mg/L BA + 2.0 mg/L 2,4-D ; and the highest regeneration rate was 100% when the callus was transferred to the medium of MS + 1.0 mg/L BA; the regenerated plantlets with fast growth, robust and stress-resistant characteristics could be obtained by inoculating the callus on the regeneration medium containing PEG-8000, and the ideal screening concentration of PEG-8000 was 5%. In this study, stress-resistant regenerated plants were obtained by directional screening of callus mutant cells, which could provide reference for plant stress-resistant breeding.

Key words: tomato, stress-resistant, mutant, PEG-8000, petiole

CLC Number:

S641.2

He Yuanqin, Xu Dingfan, Liu Yanjun, Ji Heliang, Zhao Lili, Dong Hongyu. Screening of tomato callus and acquisition of stress resistant mutants[J]. Journal of Tianjin Agricultural University, 2022, 29(3): 9-13.

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Screening of tomato callus and acquisition of stress resistant mutants

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