天津农学院学报 ›› 2026, Vol. 33 ›› Issue (2): 23-27.doi: 10.19640/j.cnki.jtau.2026.02.005

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藜麦苗提取物的抗氧化和体外降糖降脂活性研究

穆努尔·吾甫a, 刘润茜a, 郑佳a, 郝元元a, 曹高燚b, 王俊斌a,通信作者   

  1. 天津农学院 a.基础科学学院,b.农学与资源环境学院,天津 300392
  • 收稿日期:2025-03-19 出版日期:2026-04-30 发布日期:2026-05-06
  • 通讯作者: 王俊斌(1979—),男,研究员,博士,研究方向为植物活性物质的提取和鉴定。E-mail:419647768@qq.com。
  • 作者简介:穆努尔·吾甫(2002—),女,本科在读,研究方向为植物活性物质的提取和鉴定。E-mail:17660622464@163.com。
  • 基金资助:
    天津市大学生创新创业训练计划项目(202310061115); 新疆维吾尔自治区自然科学基金项目(2022D01A10); 天津市“项目+团队”重点培养专项(XC202060)

Study on antioxidant and in vitro hypoglycemic and hypolipidemic activities of extracts from quinoa seedlings

Munuera, Liu Runxia, Zheng Jiaa, Hao Yuanyuana, Cao Gaoyib, Wang Junbina,Corresponding Author   

  1. Tianjin Agricultural University, a. College of Basic Sciences, b. College of Agronomy and Resource Environment, Tianjin 300392, China
  • Received:2025-03-19 Online:2026-04-30 Published:2026-05-06

摘要: 为探究藜麦苗中活性物质的抗氧化和降血糖、降血脂的功能,采用超声波辅助醇提法对藜麦苗中的多酚进行提取,测定了提取物对DPPH自由基的清除率以及α-淀粉酶和α-葡萄糖苷酶活性的抑制率,并通过体外模拟胆酸盐结合试验评价其降脂活性。结果表明,藜麦苗提取物中含多酚2.58 mg/g,在测定范围内对DPPH自由基的清除率在35.44%~65.19%之间。藜麦苗提取物对DPPH自由基、α-淀粉酶活性和α-葡萄糖苷酶活性的半数抑制浓度(IC50)分别为0.555 mg/mL、9.599 μg/mL和13.272 μg/mL,并且抑制效果随着浓度的增加而增强。藜麦苗提取物对甘氨胆酸钠和牛磺胆酸钠的结合率随浓度的增加而增加,最大结合率分别是94.22%和75.54%。藜麦苗的多酚浓度与DPPH自由基清除率、α-淀粉酶抑制率、α-葡萄糖苷酶抑制率、甘氨胆酸钠结合率、牛磺胆酸钠结合率都达到了极显著正相关(P<0.01),表明藜麦苗中的多酚有较强的体外降糖降脂活性。

关键词: 藜麦苗, 多酚, 抗氧化, 超声辅助提取, 降糖, 降脂

Abstract: In order to explore the antioxidant, hypoglycemic and hypolipidemic effects of active substances in quinoa seedlings, ultrasonic-assisted ethanol extraction method was used to extract polyphenols from quinoa seedlings, and the DPPH free radical scavenging rate, α-amylase and α-glucosidase activities inhibition rate were determined, and the hypolipidemic activity was evaluated by in vitro simulated cholate binding experiment. The results showed that quinoa seedling extracts contained 2.58 mg/g polyphenols, and the DPPH free radical scavenging rate ranged from 35.44% to 65.19% in the determination range. The half maximal inhibitory concentrations(IC50)of quinoa seedling extract on DPPH free radicals, α-amylase activity, and α-glucosidase activity were 0.555 mg/mL, 9.599 μg/mL and 13.272 μg/mL, respectively, and the inhibitory effect increased with increasing concentration. The binding rate of quinoa seedling extracts to sodium glycocholate and sodium taurocholate increased with the increase of concentration, and the maximum binding rates were 94.22% and 75.54%, respectively. The concentration of quinoa seedling polyphenols had a very significant positive correlation with DPPH free radical scavenging rate, α-amylase inhibition rate, α-glucosidase inhibition rate, sodium glycocholate binding rate and sodium taurocholate binding rate, indicating that quinoa seedling polyphenols have strong hypoglycemic and hypolipidemic activity in vitro.

Key words: quinoa seedlings, polyphenols, antioxidation, ultrasonic assisted extraction, hypoglycemic, hypolipidemic

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