天津农学院学报 ›› 2025, Vol. 32 ›› Issue (4): 54-61.doi: 10.19640/j.cnki.jtau.2025.04.008

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银耳多糖提取工艺的优化及抗氧化活性比较

刘丹丹a, 姜迎迎a, 安娜a, 钱磊b, 张平平a, 张业尼a,通信作者   

  1. 天津农学院 a.食品科学与生物工程学院,b.农学与资源环境学院,天津 300392
  • 收稿日期:2023-09-17 发布日期:2025-09-02
  • 通讯作者: 张业尼(1984—),女,讲师,博士,研究方向为天然活性成分开发与研究。E-mail:zhangyeni@tjau.edu.cn。
  • 作者简介:刘丹丹(1999—),女,硕士在读,研究方向为天然活性成分开发与研究。E-mail:liudandan20001013@163.com。
  • 基金资助:
    天津市科技计划项目(23ZYCGSN00870、24ZYCGSN00070);甘肃省科技计划项目(24CXNH009);甘肃省中央引导地方科技发展资金项目(24ZYQH006);天津市蔬菜现代农业产业技术体系创新团队(ITTVRS2024016);农业农村部农产品贮藏保鲜重点实验室开放基金项目(Kf2021005)

Optimization of extraction process and comparison of antioxidant activity of Tremella Fuciformis polysaccharides

Liu Dandana, Jiang Yingyinga, An Naa, Qian Leib, Zhang Pingpinga, Zhang Yenia,Corresponding Author   

  1. Tianjin Agricultural University, a. College of Food Science and Biotechnology, b. College of Agronomy and Resource Environment, Tianjin 300392, China
  • Received:2023-09-17 Published:2025-09-02

摘要: 对四个不同产地的银耳多糖的抗氧化活性进行研究,明确吉林长白山银耳多糖的还原能力最强,其还原能力、羟基自由基清除能力、超氧阴离子自由基清除能力和DPPH自由基清除能力均高于其他三地的银耳多糖。在此基础上,以长白山银耳为材料,采用高温蒸汽提取法,进行单因素、响应面优化试验,确定最佳提取工艺条件:料液比为(1:68)g/mL、提取温度为121 ℃、提取时间为1.0 h,此条件下多糖提取率达(41.31±1.02)%。该提取工艺的优化使银耳多糖的提取更高效,为今后银耳产品的开发及利用奠定了理论基础。

关键词: 银耳多糖, 单因素, 响应面优化, 最佳工艺, 抗氧化活性

Abstract: A study on the antioxidant activity of Tremella fuciformis polysaccharides from four different regions demonstrated that the polysaccharides from Jilin Changbai Mountain exhibited the strongest reducing power, with superior performance in hydroxyl radical scavenging capacity, superoxide anion radical scavenging capacity, and DPPH radical scavenging capability compared to those from other three regions. Using Changbai Mountain Tremella fuciformis as materials, high-temperature steam extraction was optimized through single-factor and response surface methodology(RSM)experiments. The optimal extraction conditions were determined as: solid-liquid ratio(1:68)g/mL, extraction temperature 121℃, and extraction duration 1.0 hour. Under these conditions, the polysaccharide extraction yield reached(41.31±1.02)%. This optimized extraction process significantly improves the efficiency of Tremella polysaccharide extraction, laying a theoretical foundation for the future development and utilization of Tremella fuciformis-based products.

Key words: Tremella fuciformis polysaccharides, single factor, response surface optimization, optimal process, antioxidant activity

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