天津农学院学报 ›› 2026, Vol. 33 ›› Issue (2): 17-22.doi: 10.19640/j.cnki.jtau.2026.02.004

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马铃薯脱毒苗组培快繁影响因子的优化

王晓萌1, 吕思瑶1,孙宁1,通信作者, 齐欣2,通信作者   

  1. 1.天津农学院 农学与资源环境学院,天津 300392;
    2.天津市农业科学院,天津 300192
  • 收稿日期:2024-10-12 出版日期:2026-04-30 发布日期:2026-05-06
  • 通讯作者: 孙宁(1979—),女,高级实验师,硕士,主要从事农业生物技术方面的研究。E-mail:sning79@sina.com; 齐欣(1974—),男,副研究员,学士,主要从事农业生物技术方面的研究。E-mail:qixin0624@126.com。
  • 作者简介:王晓萌(2003—),女,本科在读,主要从事植物细胞工程与种子科学研究。E-mail:364513442@qq.com。
  • 基金资助:
    天津市蔬菜现代农业产业技术体系创新团队项目(ITTVRS2024016); 天津市大学生创新创业训练计划项目(202310061161)

Optimization of influencing factors on tissue culture and rapid propagation of virus-free potato seedlings

Wang Xiaomeng1, Lü Siyao1, Sun Ning1,Corresponding Author, Qi Xin2,Corresponding Author   

  1. 1. College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300392, China;
    2. Tianjin Academy of Agriculture Sciences, Tianjin 300192, China
  • Received:2024-10-12 Online:2026-04-30 Published:2026-05-06

摘要: 以马铃薯(Solanum tuberosum L.)品种‘费乌瑞它’为材料,研究培养基类型、外源激素、碳源、活性炭及水质等多种因素对马铃薯脱毒苗组培快繁的影响。结果表明:马铃薯脱毒苗在MS培养基上的长势优于在B5和N6培养基上的长势,但在B5培养基中单株叶片数与新生芽苗数显著高于MS培养基的同类指标;添加1~2 g/L活性炭有利于马铃薯脱毒苗的生长与增殖,平均单株叶片数与新生芽苗数分别高于未添加活性炭处理组45.7%和47.7%;添加0.5 mg/L 6-BA与0.2 mg/L NAA可有效促进马铃薯脱毒苗的生长。用20~25 g/L白砂糖代替MS培养基中的蔗糖作为碳源,在制作培养基时使用自来水,不仅有利于提高脱毒苗的质量和繁殖效率,还可有效降低生产成本。

关键词: 马铃薯, 脱毒苗, 组培快繁, 生长, 增殖

Abstract: Using potato cultivar ‘Favorita’ as the material, the effects of media type, exogenous hormone, carbon source, activated carbon and water quality on tissue culture and rapid propagation of virus-free potato seedlings were studied. The results showed that the growth of virus-free potato seedlings on MS medium was superior to that on B5 and N6 media. However, the number of leaves per plant and number of new shoots on B5 medium were significantly higher than those on MS. The addition 1-2 g/L activated carbon was beneficial to the growth and proliferation of virus-free potato seedlings, with the average number of leaves and new shoots being 45.7% and 47.7% higher than those of the control group without activated carbon respectively. The addition of 0.5 mg/L 6-BA and 0.2 mg/L NAA could effectively promote the growth of virus-free potato seedlings. Replacing sucrose in MS medium with 20-25 g/L white sugar as the carbon source, and using tap water in the preparation of medium, not only improved the quality and propagation efficiency of virus-free seedlings, but also effectively reduced the production costs.

Key words: potato, virus-free seedlings, tissue culture and rapid propagation, growth, proliferation

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