天津农学院学报 ›› 2017, Vol. 24 ›› Issue (2): 58-62.

• 研究与简报 • 上一篇    下一篇

响应面法优化热压浸提牛骨蛋白提取工艺

樊晓盼1,2, 张伯男1,2, 张甜3, 马俪珍1,2,通信作者   

  1. 1. 天津农学院 食品科学与生物工程学院,天津300384;
    2. 天津市农副产品深加工技术工程中心,天津 300384;
    3. 山西农业大学 食品科学与工程学院,山西 太谷 030801
  • 收稿日期:2016-06-02 出版日期:2017-06-30 发布日期:2019-10-15
  • 通讯作者: 马俪珍(1963-),女,山西运城人,教授,博士,研究方向:肉品科学与技术。E-mail:malizhen-6329@163.com。
  • 作者简介:樊晓盼(1993-),女,山西运城人,硕士在读,研究方向:动物源性食品安全与营养学。E-mail:308925471@qq.com。
  • 基金资助:
    天津市农业科技成果转化与推广项目“可食性牛羊骨素加工关键技术集成及工程化示范”(201604060)

Optimization High-temperature and High-pressure ExtractionProcess of Bovine Bone Protein Using Response Surface Methodology

FAN Xiao-pan1,2, ZHANG Bo-nan1,2, ZHANG Tian3, MA Li-zhen1,2,Corresponding Author   

  1. 1. College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300384, China;
    2. Tianjin Engineering and Technology Research Center of Agricultural Products Processing, Tianjin 300384, China;
    3. College of Food Science and Bioengineering, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China
  • Received:2016-06-02 Online:2017-06-30 Published:2019-10-15

摘要: 以牛骨碎屑为试验对象,通过单因素和响应面优化试验,研究热压浸提条件(料液比、浸提时间和浸提温度)对牛骨碎屑中蛋白提取率的影响,进而得出利用热压浸提技术提取牛骨蛋白的最佳条件。结果表明:料液比为1:4、浸提时间为4 h、浸提温度为133 ℃时,牛骨碎屑中的蛋白提取率可达到64.73%。

关键词: 牛骨碎屑, 蛋白, 响应面法

Abstract: In this study, bone fragments were chosen as raw material. The influence of high-temperature and high-pressure technology on the extraction rate of bovine bone protein were evaluated. Single factors and response surface methodology was used to optimize extraction of bovine protein by using high-temperature and high-pressure technology. The main parameters including the material-fluid ration, extract time and extract temperature, and the extraction rate of protein were the evaluation index. The research results showed that the optimum conditions of extraction are as follows: material-fluid ration is 1:4, extract time is 4 h and extract temperature is 133 ℃. Under the optimal extraction condition, the extraction rate of bovine bone protein was 64.73%.

Key words: bone fragments, protein, response surface methodology

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