MSTN基因敲除促进牛骨骼肌脂肪代谢

doi:10.19640/j.cnki.jtau.2022.03.009

天津农学院学报 ›› 2022, Vol. 29 ›› Issue (3): 47-51.doi: 10.19640/j.cnki.jtau.2022.03.009

MSTN基因敲除促进牛骨骼肌脂肪代谢

盛辉, 郭益文, 张林林, 李新, 丁向彬, 郭宏^通信作者

天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室,天津 300392

收稿日期:2020-09-16 出版日期:2022-09-30 发布日期:2022-10-10
通讯作者: 郭宏（1964—）,男,教授,博士,主要从事动物胚胎与转基因工程方面研究。E-mail：guohong64@163.com。
作者简介:盛辉（1996—）,男,硕士在读,主要从事动物胚胎与转基因工程方面研究。E-mail：shengjhui@163.com。
基金资助:
国家重大专项（2016ZX08007-002）

Study on fat metabolism of bovine skeletal muscle by MSTN gene

Sheng Hui, Guo Yiwen, Zhang Linlin, Li Xin, Din Xiangbin, Guo Hong^{Corresponding Author}

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China

Received:2020-09-16 Online:2022-09-30 Published:2022-10-10

摘要/Abstract

摘要： 为探究肌肉生长抑制素（MSTN）对骨骼肌脂肪代谢的影响,采集MSTN^-/-蒙古牛（MG.ZJY）与野生蒙古牛（MG.DZ）、MSTN^-/-鲁西黄牛（LX.ZJY）与野生鲁西黄牛（LX.DZ）和MSTN^-/-蒙古黑牛（HN.ZJY）与野生蒙古黑牛（HN.DZ）腿臀肌肌肉组织为样品,运用定量蛋白质组学与磷酸化蛋白质组学技术对差异蛋白和差异磷酸化蛋白进行筛选,通过在线生物信息学分析软件与网站对这些差异蛋白和磷酸化蛋白进行统计和分析,并检测各个组织中甘油三酯含量。结果显示,鉴定到的多个差异蛋白都与脂肪代谢相关,这些差异蛋白主要富集到脂肪酸降解、脂肪酸代谢和PPAR信号通路。组织甘油三酯测定发现,与野生牛相比,MSTN^-/-牛肌肉组织中甘油三酯含量显著下降。本研究结果表明,敲除MSTN基因能够促进牛骨骼肌脂肪酸代谢。研究结果为进一步开展MSTN基因对骨骼肌代谢机制影响的研究奠定了基础。

关键词: MSTN, 骨骼肌, 蛋白质组学, 脂肪酸代谢

Abstract: To investigate the effect of myostatin（MSTN）on skeletal muscle fat metabolism, MSTN^-/-Mongolian cattle （MG.ZJY）and wild Mongolian cattle（MG.DZ）, MSTN^-/- Luxi cattle（LX.ZJY）and wild Luxi cattle（LX.DZ）and MSTN^-/- Mongolian Black Cattle（HN.ZJY）and wild Mongolian black cattle（HN.DZ）leg gluteal muscle tissue as a sample, quantitative proteomics and phosphorus Acidified proteomics technology were used to screen differential proteins and differentially phosphorylated proteins. These differential proteins and phosphorylated proteins were statistically analyzed by online bioinformatics analysis software and website, and triglyceride content in various tissues were detected. The results showed that the identified multiple differential proteins were all related to fat metabolism, and these differential proteins were mainly enriched in fatty acid degradation, fatty acid metabolism and PPAR signaling pathways. Tissue triglyceride assay showed that the triglyceride content in muscle tissue of MSTN^-/- cattle was significantly decreased compared with that of wild cattle. The results of this study showed that knockout of MSTN gene could promote fatty acid metabolism in bovine skeletal muscle. The results lay a foundation for further research on the effect of MSTN gene on skeletal muscle metabolic mechanism.

Key words: MSTN, skeletal muscle, proteomics, fatty acid metabolism

中图分类号:

S813.3

盛辉, 郭益文, 张林林, 李新, 丁向彬, 郭宏. MSTN基因敲除促进牛骨骼肌脂肪代谢[J]. 天津农学院学报, 2022, 29(3): 47-51.

Sheng Hui, Guo Yiwen, Zhang Linlin, Li Xin, Din Xiangbin, Guo Hong. Study on fat metabolism of bovine skeletal muscle by MSTN gene[J]. Journal of Tianjin Agricultural University, 2022, 29(3): 47-51.

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http://xuebao.tjau.edu.cn/CN/Y2022/V29/I3/47

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MSTN基因敲除促进牛骨骼肌脂肪代谢

Study on fat metabolism of bovine skeletal muscle by MSTN gene

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