溶解氧对半滑舌鳎呼吸频率、生理生化指标和组织结构的影响

doi:10.19640/j.cnki.jtau.2024.01.010

天津农学院学报 ›› 2024, Vol. 31 ›› Issue (1): 54-60.doi: 10.19640/j.cnki.jtau.2024.01.010

溶解氧对半滑舌鳎呼吸频率、生理生化指标和组织结构的影响

聂国梁¹, 王庆奎^{1,通信作者}, 赵鸿昊¹, 王祯辉², 付建东², 李强^{1,通信作者}

1.天津农学院水产学院,天津 300392;
2.天津市海发珍品实业发展有限公司,天津 300450

收稿日期:2023-05-11 出版日期:2024-02-29 发布日期:2024-04-02
通讯作者: 王庆奎（1978—）,男,教授,博士,主要从事海水鱼循环水健康养殖方面研究。E-mail：wangqk@tjau.edu.cn。李强（1979—）,男,教授,博士,研究方向：水产动物病害快速诊断与免疫防控。E-mail：liqiangtn@tjau.edu.cn。
作者简介:聂国梁（1996—）,男,硕士在读,主要从事海水鱼循环水健康养殖方面研究。E-mail：1020482081@qq.com。
基金资助:
现代农业产业技术体系专项资金（CARS-47）; 天津市海水养殖产业技术体系创新团队（ITTMRS2021000）; 国家重点研发计划“蓝色粮仓科技创新”专项（2020YFD0900600）; 天津市高等学校“创新团队培养计划”（TD13-5089）

Effects of dissolved oxygen on respiratory rate, physiological and biochemical parameter and tissue structure of Cynoglossus semilaevis

Nie Guoliang¹, Wang Qingkui^{1,Corresponding Author}, Zhao Honghao¹, Wang Zhenhui², Fu Jiandong², Li Qiang^{1,Corresponding Author}

1. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Haifa Zhenpin Industrial Development Co., Ltd., Tianjin 300450, China

Received:2023-05-11 Online:2024-02-29 Published:2024-04-02

摘要/Abstract

摘要： 在循环水养殖系统中由于常出现低氧和高氧的情况,为了解溶解氧变化对半滑舌鳎（Cynoglossus semilaevis）的影响,本试验检测了低溶解氧（3 mg/L）、对照组（8 mg/L）、高溶解氧（14 mg/L、20 mg/L）条件下半滑舌鳎呼吸、生理生化指标和组织结构的情况。结果表明,低氧组半滑舌鳎在24 h 内呼吸频率显著增加,而高氧组呼吸频率显著降低（P<0.05）。在第七天低氧组呼吸频率与对照组无显著差异（P>0.05）,而高氧组呼吸频率随溶解氧升高而显著降低（P<0.05）。第十四天后,低氧组呼吸频率与对照组差异不显著,而高氧组呼吸频率随溶解氧升高而显著降低（P<0.05）。低氧组和高氧组较对照组均出现上皮细胞层增生现象,高氧组半滑舌鳎鳃组织较对照组出现较多空泡现象。低氧组肝脏组织结构异常,肝细胞广泛疏松水肿,细胞轮廓不清晰,而高氧组肝细胞轮廓清晰,胞浆红染,未见明显变性。低氧组血液红细胞数量、血红蛋白含量较对照组增高（P>0.05）,红细胞比积较对照组减少（P>0.05）。高氧组红细胞数量、血红蛋白含量和红细胞比积均高于对照组（P>0.05）。低氧组和高氧组血浆MDA含量、SOD活力及CAT活力均显著高于对照组（P<0.05）。结论低氧和高氧都对半滑舌鳎产生胁迫,低氧对半滑舌鳎的胁迫更明显。

关键词: 半滑舌鳎, 溶解氧, 呼吸频率, 血液指标, 组织切片, 酶活性

Abstract: In order to understand the effects of dissolved oxygen changes on Cynoglossus semilaevis , which often occurs in circulating water culture systems with low and high oxygen, this experiment examined the effects of low dissolved oxygen（3 mg/L）, control（8 mg/L）, and high dissolved oxygen（14 mg/L, 20 mg/L）conditions on the respiration, physiological and biochemical parameter, and tissue structure of Cynoglossus semilaevis. The results showed that respiratory rate increased significantly in the hypoxic group and decreased significantly in the hyperoxic group within 24 h（P<0.05）At day 7, the respiratory rate of the low oxygen group was not significantly different from that of the control group（P>0.05）, while the respiratory rate of the high oxygen group decreased significantly with the increase of dissolved oxygen（P<0.05）. After the 14th day, the respiratory rate of the low oxygen group was not significantly different from that of the control group, while the respiratory rate of the high oxygen group decreased significantly with the increase of dissolved oxygen（P<0.05）. Both the hypoxic and hyperoxic groups showed proliferation of epithelial cell layers compared to the control group, and the gill tissue in the hyperoxic group showed more vacuolation than the control group in the Cynoglossus semilaevis. In the hypoxic group, liver tissue structure was abnormal, with extensive loose edema and unclear cell outline, while the hepatocytes in the hyperoxic group had clear outline and red stained cytoplasm, but no obvious degeneration was observed. The number of erythrocytes and hemoglobin content of blood in the hypoxic group increased compared with the control group（P>0.05）, and the ratio of erythrocyte volume decreased compared with the control group（P>0.05）. The erythrocyte count, hemoglobin content and erythrocyte specific volume were higher in the hyperoxia group than in the control group（P>0.05）. Plasma MDA content, SOD activity and CAT activity were significantly higher in the hypoxic and hyperoxic groups than in the control group（P<0.05）. In conclusion, both hypoxia and hyperoxia caused stress on Cynoglossus semilaevis, and the stress of hypoxia was more obvious on Cynoglossus semilaevis.

Key words: Cynoglossus semilaevis, dissolved oxygen, respiratory rate, blood indicators, tissue sections, enzyme activity

中图分类号:

S917.4

聂国梁, 王庆奎, 赵鸿昊, 王祯辉, 付建东, 李强. 溶解氧对半滑舌鳎呼吸频率、生理生化指标和组织结构的影响[J]. 天津农学院学报, 2024, 31(1): 54-60.

Nie Guoliang, Wang Qingkui, Zhao Honghao, Wang Zhenhui, Fu Jiandong, Li Qiang. Effects of dissolved oxygen on respiratory rate, physiological and biochemical parameter and tissue structure of Cynoglossus semilaevis[J]. Journal of Tianjin Agricultural University, 2024, 31(1): 54-60.

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溶解氧对半滑舌鳎呼吸频率、生理生化指标和组织结构的影响

Effects of dissolved oxygen on respiratory rate, physiological and biochemical parameter and tissue structure of Cynoglossus semilaevis

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