鱼腥草添加对人工感染维氏气单胞菌锦鲤鳃部部分免疫指标的影响

doi:10.19640/j.cnki.jtau.2024.01.011

天津农学院学报 ›› 2024, Vol. 31 ›› Issue (1): 61-65.doi: 10.19640/j.cnki.jtau.2024.01.011

鱼腥草添加对人工感染维氏气单胞菌锦鲤鳃部部分免疫指标的影响

吕兆君¹, 尤禄涵², 仇钧仪¹, 李建胜³, 闫学成¹, 陈成勋^{1,通信作者}

1.天津农学院水产学院天津市水产生态及养殖重点实验室,天津 300392;
2.Indian Springs School,Alabama 35124;
3.宁河区农村社会事业发展服务中心,天津 301500

收稿日期:2023-05-08 出版日期:2024-02-29 发布日期:2024-04-02
通讯作者: 陈成勋（1967—）,男,研究员,学士,主要从事水产养殖及养殖鱼类环境改善研究。E-mail：ccxnxy@163.com。
作者简介:吕兆君（1996—）,女,硕士在读,主要从事水产增养殖研究。E-mail：761417247@qq.com。
基金资助:
天津市淡水养殖产业技术体系创新团队建设项目（ITTFRS2021000）; 天津市宝坻区农业科技计划项目（202005）; 天津市科技计划项目（22ZYCGSN00240）; 甘肃省科技计划项目（22CX8NE208）; 天津市科技计划项目（23ZYCGSN00780）

Effects of Houttuynia cordata Thunb supplementation on some immune indices in the gills of Koi carp artificially infected with Aeromonas veronii

Lü Zhaojun¹, You Luhan（Coco）², Qiu Junyi¹, Li Jiansheng³, Yan Xuecheng¹, Chen Chengxun^{1,Corresponding Author}

1. Tianjin key laboratory of Aquatic Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
2. Indian Springs School, Alabama 35124, America;
3. Ninghe District Rural Social Enterprise Development Service Center, Tianjin 301500, China

Received:2023-05-08 Online:2024-02-29 Published:2024-04-02

摘要/Abstract

摘要： 为探讨鱼腥草对人工感染维氏气单胞菌锦鲤鳃部部分免疫指标的影响,选取锦鲤96尾随机分两组,C组锦鲤投喂添加6‰鱼腥草的饲料,K组投喂基础日粮作为对照。分别饲养14 d后空腹24 h,通过腹腔进行人工感染维氏气单胞菌,于0、1、2、4 d进行采样,检测鳃部的炎性因子（Il-4,Il-10）和免疫球蛋白（Ig T）。结果表明：K组四个时间段IL-4、IL-10和IgT含量差异均不显著（P>0.05）;C组IL-4含量1 d和2 d时显著高于0 d、4 d（P<0.05）,且高于K组;IL-10含量1,2,4 d时显著高于0 d（P<0.05）,且均高于K组;IgT含量1 d与2 d高于4 d且显著高于0 d（P<0.05）,且1 d与2 d时C组高于K组。说明前期鱼腥草饲养对细菌感染锦鲤鳃部免疫具有一定的提升作用。

关键词: 鱼腥草, 锦鲤, IL-4, IL-10, IgT

Abstract: To investigate the effects of Houttuynia cordata Thunb on some immune indexes in the gill of Koi carp artificially infected with Aeromonas veronii, ninety-six koi were randomly divided into two groups. Group C was fed with the diet supplemented with 6‰ Houttuynia cordata Thunb, and group K was fed with the basal diet as the control. After 14 days of feeding, Aeromonas veronii was artificially infected through the abdominal cavity on an empty stomach for 24 h. Samples were collected at 0, 1, 2, 4 days to detect inflammatory factors（Il-4, Il-10）and immunoglobulin（IgT）in the gill. The results showed as follows: there were no significant differences in the contents of IL-4, IL-10 and IgT in group K at four time periods（P>0.05）. IL-4 content in group C was significantly higher than that in group 0 and 4 at 1 and 2 days（P<0.05）, and was higher than that in group K; IL-10 contents at 1, 2 and 4 d was were significantly higher than that at 0 d（P<0.05）, and was higher than that in K group. The IgT content at 1 d and 2 d was higher than that at 4 d and was significantly higher than that at 0 d（P<0.05）and the IgT content in group C was higher than that in group K at 1 d and 2 d. These results indicated that Houttuynia cordata Thunb feeding could improve the gill immunity of bacterial infected koi carp.

Key words: Houttuynia cordata Thunb, Koi carp, IL-4, IL-10, IgT

中图分类号:

S965

吕兆君, 尤禄涵, 仇钧仪, 李建胜, 闫学成, 陈成勋. 鱼腥草添加对人工感染维氏气单胞菌锦鲤鳃部部分免疫指标的影响[J]. 天津农学院学报, 2024, 31(1): 61-65.

Lü Zhaojun, You Luhan（Coco）, Qiu Junyi, Li Jiansheng, Yan Xuecheng, Chen Chengxun. Effects of Houttuynia cordata Thunb supplementation on some immune indices in the gills of Koi carp artificially infected with Aeromonas veronii[J]. Journal of Tianjin Agricultural University, 2024, 31(1): 61-65.

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http://xuebao.tjau.edu.cn/CN/Y2024/V31/I1/61

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鱼腥草添加对人工感染维氏气单胞菌锦鲤鳃部部分免疫指标的影响

Effects of Houttuynia cordata Thunb supplementation on some immune indices in the gills of Koi carp artificially infected with Aeromonas veronii

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