镰形艾美耳球虫感染早期对小鼠的致病性研究

doi:10.19640/j.cnki.jtau.2018.01.012

天津农学院学报 ›› 2018, Vol. 25 ›› Issue (1): 54-58.doi: 10.19640/j.cnki.jtau.2018.01.012

镰形艾美耳球虫感染早期对小鼠的致病性研究

贾妮娜, 袁顺子, 车丽霞, 张丽萍, 杨雯, 任超^通信作者

天津农学院动物科学与动物医学学院,天津 300384

收稿日期:2017-04-25 出版日期:2018-03-20 发布日期:2019-11-05
通讯作者: 任超（1986-）,女,实验师,硕士,研究方向为基础兽医学。E-mail：chaoren04050110@163.com。
作者简介:贾妮娜（1996-）,女,本科在读,研究方向为基础兽医学。E-mail：820369011@qq.com。
基金资助:
大学生创新创业训练计划项目（201610061008,201710061045）; 天津农学院高校教师教育改革创新引导发展项目（20171003）; 国家自然科学基金重点项目（31330076）

On the pathogenicity of early infection of Eimeria falciformis in mice

JIA Ni-na, YUAN Shun-zi, CHE Li-xia, ZHANG Li-ping, YANG Wen, REN Chao^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2017-04-25 Online:2018-03-20 Published:2019-11-05

摘要/Abstract

摘要： 为研究镰形艾美耳球虫感染早期对小鼠的致病性,将镰形艾美耳球虫经口感染小鼠,同时设立无球虫感染空白对照组。通过观察各组小鼠的临床症状,感染36 h后剖检的眼观病变,以及各个脏器的病理组织学观察和盲肠免疫组化观察,来研究感染早期镰形艾美耳球虫对小鼠的致病性。结果表明：早期感染镰形艾美耳球虫主要破坏肠道的黏膜组织,导致黏膜上皮和腺上皮细胞变性、坏死、脱落,黏膜下层水肿,炎性细胞浸润至黏膜层,对其他脏器未造成可见的病理损伤;免疫组化观察,发现早期感染镰形艾美耳球虫也主要侵染黏膜层和黏膜下层。因此,镰形艾美耳球虫仅感染小鼠的盲肠,不感染其他器官,可以作为候选的疫苗载体。

关键词: 镰形艾美耳球虫, 致病性, 小鼠, 器官指数, 免疫组化, 病理组织切片

Abstract: In order to study the pathogenicity of early infection of Eimeria falciformis in mice, E. falciformis was invading by oral infection in the treatment group, while the control group was established with non-coccidia infection. Then the clinical symptoms, autopsy lesions, organ index, organ pathological histology and immunohistochemical characterization of cecum were observed for 36 hours to learn about the pathogenicity of early infection of E. falciformis in mice. The results showed that in the early stage of infection, E. falciformis maily damaged mucosal tissues of intestinal tract which caused degeneration, necrosis and shedding of epithelial and glandular epithelial cells, edema in strata submucosum, inflammatory cells infiltrating the mucosa but no visible pathological damage to other organs. According to immunohis chemical observation, the infections of mucosa and submucosa in the early stage were also observed. Hence, E. falciformis will be a candidate vaccine vector, which did not damage all the organs except cecum.

Key words: Eimeria falciformis, pathogenicity, mice, organ index, immunohistochemistry, histopathological sections

中图分类号:

S852.72⁺3

贾妮娜, 袁顺子, 车丽霞, 张丽萍, 杨雯, 任超. 镰形艾美耳球虫感染早期对小鼠的致病性研究[J]. 天津农学院学报, 2018, 25(1): 54-58.

JIA Ni-na, YUAN Shun-zi, CHE Li-xia, ZHANG Li-ping, YANG Wen, REN Chao. On the pathogenicity of early infection of Eimeria falciformis in mice[J]. Journal of Tianjin Agricultural University, 2018, 25(1): 54-58.

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http://xuebao.tjau.edu.cn/CN/Y2018/V25/I1/54

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镰形艾美耳球虫感染早期对小鼠的致病性研究

On the pathogenicity of early infection of Eimeria falciformis in mice

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