关键词: 樱桃李, 休眠芽, 赤霉素, 茎段, 灭菌, 培养

Abstract: This paper aimed at researching on the hastening effect of GA₃ on dormant buds germination and on the sterilizing and culturing technique of stem sections of Prunus cerasifera. The annual dormant shoot of Prunus cerasifera taken in the first ten-day period of December and middle ten-day period of January were soaked in GA₃ solution and then cultured in water for bud busting. The stem sections of Prunus cerasifera were sterilized with alcohol and sodium hypochlorite and then inoculated in different media. The result showed that soaked in GA₃ (100 mg/L or 200 mg/L) solution for 1 or 2 hours the dormant buds of the Prunus cerasifera germinated obviously earlier and the shoot was marvelously longer compared with the control soaked in water. For annual dormant shoot taken in the first ten-day period of December the treatment of soaked in GA₃ (100 mg/L or 200 mg/L) solution for 2 hours, the germination rate increased obviously. After soaked in alcohol not only the young shoot from Prunus cerasifera dormant buds germinating cultured in water were soaked in sodium hypochlorite (2%) solution for 6-9 minutes but also the stem section of Prunus cerasifera taken in field in spring were soaked in sodium hypochlorite (2%) solution for 10-25 minutes. The both obtained the same result that the pollution rate was near to 0 and the survival rate was 100%. In MS medium added 0.5 mg/L of 6-BA the young shoot from Prunus cerasifera dormant buds germinating cultured in water had the germination rate of 71.4% while the stem section of Prunus cerasifera taken in field in spring had the germination rate of 80.0%-95.5%. These results can supply reference for prolonging the period when the Prunus cerasifera explants were taken and perfecting the sterilizing and culturing technique of the Prunus cerasifera explants.

Key words: Prunus cerasifera, dormant buds, gibberellin, stem section, sterilizing, culturing