纤细裸藻共栖细菌的分离鉴定及其对藻细胞生长的影响

doi:10.19640/j.cnki.jtau.2022.02.012

天津农学院学报 ›› 2022, Vol. 29 ›› Issue (2): 57-62.doi: 10.19640/j.cnki.jtau.2022.02.012

纤细裸藻共栖细菌的分离鉴定及其对藻细胞生长的影响

汪笑宇¹, 任舒天², 吴雅婷², 武尊², 高浩峰², 邵蓬^{2,通信作者}

1.天津市水产研究所,天津 300221;
2.天津农学院水产学院,天津 300392

收稿日期:2022-05-01 出版日期:2022-06-30 发布日期:2022-07-05
通讯作者: 邵蓬（1982—）,男,副教授,硕士,主要从事水产微生物及微藻方向研究。E-mail：shaopeng@tjau.edu.cn。
作者简介:汪笑宇（1983—）,女,助理研究员,硕士,主要从事水产微生物病原学方向的研究。E-mail：smallouc@163.com。
基金资助:
天津市科技支撑项目（18YFZCNC01190）

Isolation of symbiotic bacteria of Euglena gracilis and effects on the growth of algal cells

Wang Xiaoyu¹, Ren Shutian², Wu Yating², Wu Zun², Gao Haofeng², Shao Peng^{2,Corresponding Author}

1. Tianjin Fisheries Research Institute, Tianjin 300221, China;
2. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2022-05-01 Online:2022-06-30 Published:2022-07-05

摘要/Abstract

摘要： 以纤细裸藻（Euglena gracilis）为研究对象,对纤细裸藻共栖细菌进行分离及鉴定,并探究可培养共栖细菌对纤细裸藻生长的影响。采用平板划线法和16S rDNA基因测序对藻共栖细菌进行分离鉴定,得到5种纤细裸藻的可培养藻际细菌,E1-Paraburkholderia fungorum、E3- Methylobacterium radiotolerans、E5-Sphingomonas dokdonensis、E6-Ralstonia insidiosa和ET2-Leifsonia soli,分别属于α-变形菌纲、β-变形菌纲和放线菌门三大门类。将纤细裸藻分别与五株菌共培养,通过测定纤细裸藻的藻细胞密度与叶绿素a含量,发现E1、E3与E5对纤细裸藻的生长均有促进作用,其中E5的促进效果最好;E5初始接种浓度为10⁷cfu/mL、纤细裸藻初始藻细胞密度为1×10⁵ cells/mL时对纤细裸藻的生长促进效果最佳。

关键词: 纤细裸藻, 藻际细菌, 藻菌共培养

Abstract: The symbiotic bacteria of Euglenum gracilis were isolated, and the influence of symbiotic bacteria on the growth of Euglenum gracilis was studied. The symbiotic bacteria were isolated by plate scribing and identified by 16S rDNA gene sequencing. Five species of symbiotic bacteria were obtained, E1-Paraburkholderia fungorum, E3-Methylobacterium radiotolerans, E5-Sphingomonas dokdonensis, E6-Ralstonia insidiosa and ET2-Leifsonia soli, which belong to α-Proteobacteria, β-Proteobacteria and Actinobacteria, respectively. The results demonstrated that E1, E3 and E5 could enhance the growth of Euglenum gracilis by measuring the cell density and chlorophyll content of algae in co-culture systems of bacteria and algae, and that E5 had the best effect. The promoting effect of Euglena gracilis was best when initial inoculation concentration of E5 bacterium was 10⁷cfu/mL and the initial cell density of Euglena gracilis was 1×10⁵ cells/mL.

Key words: Euglena gracilis, symbiotic bacteria, co-culture of bacteria and algae

中图分类号:

S917.1

汪笑宇, 任舒天, 吴雅婷, 武尊, 高浩峰, 邵蓬. 纤细裸藻共栖细菌的分离鉴定及其对藻细胞生长的影响[J]. 天津农学院学报, 2022, 29(2): 57-62.

Wang Xiaoyu, Ren Shutian, Wu Yating, Wu Zun, Gao Haofeng, Shao Peng. Isolation of symbiotic bacteria of Euglena gracilis and effects on the growth of algal cells[J]. Journal of Tianjin Agricultural University, 2022, 29(2): 57-62.

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链接本文: http://xuebao.tjau.edu.cn/CN/10.19640/j.cnki.jtau.2022.02.012

http://xuebao.tjau.edu.cn/CN/Y2022/V29/I2/57

参考文献

[1] SARMENTO H,GASOL J M.Use of phytoplankton-derived dissolved organic carbon by different types of bacterioplankton[J]. Environmental Microbiology,2012,14(9):2348-2360.
[2] 高亚辉. 海洋微藻分类生态及生物活性物质研究[J]. 厦门大学学报(自然科学版),2001,40(2):566-573.
[3] FUJITA T,AOYAGI H,OGBONNA J C,et al.Effect of mixed organic substrate on α-tocopherol production by Euglena gracilisin photoheterotrophic culture[J]. Applied Microbiology & Biotechnology,2008,79(3):371-378.
[4] NOVAK M,VETVICKA V.β-Glucans,history,and the present:immunomodulatory aspects and mechanisms of action[J]. Journal of Immunotoxicology,2008,20(5):47-57.
[5] IRINA M,IVANOVA,SERGEY A,et a1. Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sagar-1-phosphate transferase activities in Euglena gracilis membranes[J]. Carbohydrate Research,2017,483:26-38.
[6] 张文慧,窦勇,高金伟,等. 纤细裸藻对5种抗生素的胁迫响应[J]. 水生态学杂志,2017,38(6):48-56.
[7] KAZAMIA E,CZESNICK H,NGUYEN T V,et al.Mutualistic interactions between vitamin B12-dependent algae and heterotrophic bacteria exhibit regulation[J]. Environmental Microbiology,2012,14(483):1466-1476.
[8] FALKOWSKI P G,FENCHEL T,DELONG E F.The microbial enginesthat drive earth’s biogeochemical cycles[J]. Science,2008,320(5879):1034-1039.
[9] CHEVANTON I,GARNIER M,BOUGARAN G,et al.Screening and selection of growth-promoting bacteria for Dunaliella cultures[J]. Algal Research,2013,2(3):212-222.
[10] CHO D H,RAMANAN R,HEO J,et al.Enhancing microalgal biomass productivity by engineering a microalgal- bacterial community[J]. Bioresource Technology,2015,175(7):578-585.
[11] 黄振华,陈星星,柯爱英,等. 2种海洋饵料微藻藻际环境的细菌多样性研究[J]. 水生态学杂志,2018,39(1):44-49.
[12] STEVEN S H,STOBNER M,SIMON M,et al.Phylogeny of Proteobacteria and Bacteroidetes from oxic habitats of a tidal flat ecosystem[J]. FEMS Microbiology Ecology,2005,54(7):351-365.
[13] KIM S J,MOON J Y,LIM J M,et al.Sphingomonas aerophila sp. nov. and Sphingomonas naasensis sp. nov. isolated from air and soil,respectively[J]. International Journal of Systematic & Evolutionary Microbiology,2014,64(3):926-932.
[14] HUY H,JIN L,LEE K C,et al.Sphingomonas daechungensis sp. nov.,isolated from sediment of a eutrophic reservoir[J]. Int J Syst Evol Microbiol,2014,64(8):1412-1418.
[15] PARK H K,HAN J H,KIM T S,et al.Sphingomonas aeria sp. nov. from indoor air of a pharmaceutical environment[J]. Antonie van Leeuwenhoek,2015,107(1):47-53.
[16] KIM Y J,LIM J,SUKWEENADHI J,et al.Genomic characterization of a newly isolated Rhizobacteria Sphingomonas panacis reveals plant growth promoting effect to rice[J]. Biotechnology and Bioprocess Engineering,2019,24(2):119-125.
[17] JEON M S,OH J J,Kim J Y,et al.Enhancement of growth and paramylon production of Euglena gracilis by co-cultivation with Pseudoalteromonas sp. MEBiC 03485[J]. Bioresource Technology,2019,288(9):1-8.
[18] LEE C,JEON M S,KIM J Y,et al.Effects of an auxin-producing symbiotic bacterium on cell growth of the microalga Haematococcus pluvialis:Elevation of cell density and prolongation of exponential stage[J]. Algal Research,2019,41(8):1-8.

纤细裸藻共栖细菌的分离鉴定及其对藻细胞生长的影响

Isolation of symbiotic bacteria of Euglena gracilis and effects on the growth of algal cells

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