玉米素灭菌方式和浓度对蓝莓试管苗增殖的影响

doi:10.19640/j.cnki.jtau.2019.01.001

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (1): 1-5.doi: 10.19640/j.cnki.jtau.2019.01.001

• Researches and Scientific Notes • Next Articles

Effect of sterilizing method and concentration of ZT on blueberry plantlet in Vitro proliferation

CHAI Ci-jiang¹, WANG Kun-ying¹, ZHANG Wen-wen¹, LIU Chun-peng², WEI Wei¹, ZOU Hang¹

1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China;
2.“ZEPENGManor” Blueberry Plantation, “ZEPENG”Agricultural Science and Technique Limited Company, Jizhou District, Tianjin 301900, China;

Received:2018-09-17 Online:2019-03-20 Published:2019-10-09

Abstract

Abstract: Both methods of ZT sterilized by high temperature and ZT sterilized by filteringwere respectively done inmaking media for blueberry plantlet in vitro proliferation to research on the effect of the sterilizing method on ZT function. The stem sections of blueberry plantlet in vitro were respectively inoculated in WPM media containing different concentration of ZT for shoot proliferation. The results showed that with the ZT concentration of 1.0 mg/L or 2.0 mg/L there were no significant difference in coefficient of multiplication of blueberry plantlet in vitro between methods of ZT sterilized by high temperature and ZT sterilized by filtering, and sterilizing with high temperature had no obvious unfavorable effect on ZT function. For blueberry varieties（‘Bluecrop’‘Northland’and‘Patriot’） the treatment of 0.5 mg/L ZT obtained the higher coefficient of multiplication among ZT concentration of 0.5-3.0 mg/L, and then the suitable multiplication medium for the three blueberry varieties was WPM+ZT 0.5 mg/L.

Key words: ZT, sterilizing with high temperature, concentration, blueberry plantlet in vitro, multiplication

CLC Number:

CHAI Ci-jiang, WANG Kun-ying, ZHANG Wen-wen, LIU Chun-peng, WEI Wei, ZOU Hang. Effect of sterilizing method and concentration of ZT on blueberry plantlet in Vitro proliferation[J]. Journal of Tianjin Agricultural University, 2019, 26(1): 1-5.

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Effect of sterilizing method and concentration of ZT on blueberry plantlet in Vitro proliferation

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