微囊藻毒素对凡纳滨对虾急性毒性的研究

doi:10.19640/j.cnki.jtau.2022.04.007

Journal of Tianjin Agricultural University ›› 2022, Vol. 29 ›› Issue (4): 31-35.doi: 10.19640/j.cnki.jtau.2022.04.007

• Researches and Scientific Notes • Previous Articles Next Articles

Study on acute toxicity of microcystins to Litopenaeus vannamei

Huang Lanying^1,2, Zhang Dajuan^1,2, Zhang Shulin^{1,2,Corresponding Author}, Ma Chenghong^1,2, Huang Ying^1,2

1. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
2. Tianjin Key Laboratory of Aquatic Ecology and Aquaculture, Tianjin 300392, China

Received:2021-02-05 Online:2022-12-30 Published:2023-01-03

Abstract

Abstract: To study the acute toxicity of MC-LR to Litopenaeus vannamei and provide reference for scientific culture of Litopenaeus vannamei, the research was carried out. With Litopenaeus vannamei as experimental material, the death individuals were recorded by injecting MC-LR at 0, 50, 100 and 200 μg/kg respectively, and the mortality and LC₅₀ of Litopenaeus vannamei under different concentrations of MC-LR injection were calculated to observe its acute toxic effects. The results showed that the LC₅₀ of MC-LR to Litopenaeus vannamei for 24 h, 48 h, 72 h and 96 h were 439.990, 384.655, 346.659 and 320.663 μg/kg respectively. The tolerance of Litopenaeus vannamei to MC-LR wasstrong, and the tolerance of Litopenaeus vannamei to MC-LR was stronger when it was infected with low concentration MC-LR. This study has certain significance for understanding the influence of MC-LR on different individual sizes of Litopenaeus vannamei and its toxic mechanism.

Key words: MC-LR, Litopenaeus vannamei, semilethal concentration

CLC Number:

S948

Huang Lanying, Zhang Dajuan, Zhang Shulin, Ma Chenghong, Huang Ying. Study on acute toxicity of microcystins to Litopenaeus vannamei[J]. Journal of Tianjin Agricultural University, 2022, 29(4): 31-35.

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References

[1] 王静. 我国南美白对虾养殖的经济效益分析[D]. 上海:上海海洋大学,2019.
[2] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会. 中国渔业统计年鉴2020[M]. 北京:中国农业出版社,2020.
[3] 闫海,潘纲,张明明. 微囊藻毒素研究进展[J]. 生态学报,2002,22(11):1968-1975.
[4] 陈妍妍. 虾池蓝藻群落及微囊藻毒素对凡纳滨对虾相关免疫酶影响[D]. 湛江:广东海洋大学,2016.
[5] 贺燕,黄先智,丁晓雯. 微囊藻毒素毒性及其作用机理研究进展[J]. 食品科学,2020,41(5):290-298.
[6] SVIRCEV Z,DROBAC D,TOKODI N,et al.Epidemiology of primary liver cancer in Serbia and possible connection with cyanobacterial blooms[J]. Journal of Environmental Science and Health,Part C,2013,31(3):181-200.
[7] 陆开宏,张春景,梁晶晶,等. 产毒微囊藻喂食暴露对铜锈环棱螺肝组织超微结构的影响[C]//中国海洋湖沼学会藻类学分会. 庆祝中国藻类学会成立30周年暨第十五次学术讨论会摘要集. 珠海:中国海洋湖沼学会,2009:166.
[8] 李智,傅一鸣,李响,等. 凡纳滨对虾在微囊藻毒素(MC-LR)诱导下3种抗菌肽基因的表达量分析[J]. 上海海洋大学学报,2014,23(6):842-847.
[9] WANG Q,XIE P,LIANG G.Distribution of microcystins in various organs(heart,liver,intestine,gonad,brain,kidney and lung)of wistar rat via intravenous injection[J]. Toxicon,2008,52(6):721-727.
[10] XIE L,XIE P,GUO L,et al.Organ distribution and bioaccumulation of microcystins in freshwater fish at different trophic levels from the eutrophic Lake Chaohu,China[J]. Environmental Toxicology,2005,20(3):293-300.
[11] 查广才,周昌清,牛晓光. 铜绿微囊藻对凡纳滨对虾低盐度养殖的危害研究[J]. 中山大学学报(自然科学版),2007,46(2):64-67.
[12] SAQRANE S,GHAZALI I E,OUAHID Y,et al.Phytotoxic effects of cyanobacteria extract on the aquatic plant Lemna gibba: microcystin accumulation,detoxication and oxidative stress induction[J]. Aquatic Toxicology,2007,83(4):284-294.
[13] SONG L R,CHEN W.Production of microcystins in bloom-forming cyanobacteria and their environmental fates:a review[J]. Journal of Lake Sciences,2009,21(6):749-757.
[14] ZURAWELL R W,CHEN H,BURKE J M,et al.Hepatotoxic cyanobacteria: A review of the biological importance of microcystins in freshwater environments[J]. Journal of Toxicology and Environmental Health,2005,8(1):1-37.
[15] JONATHAN P,MICHÈLE R P,SUSANNA A W,et al. High levels of structural diversity observed in microcystins from Microcystis CAWBG11 and characterization of six new microcystin congeners[J]. Marine Drugs,2014,12(11):5372-5395.
[16] MALBROUCK C,KESTEMONT P.Effects of microcystins on fish[J]. Environmental Toxicology and Chemistry,2006,25(1):72-86.
[17] 张学振. 微囊藻毒素对鱼类和哺乳动物致毒效应的比较研究[D]. 武汉:华中农业大学,2008.
[18] GONÇALVES-SOARES D,ZANETTE J,YUNES J S,et al. Expression and activity of glutathione S-transferases and catalase in the shrimp Litopenaeus vannamei inoculated with a toxic Microcystis aeruginosa strain[J]. Marine Environmental Research,2012,75:54-61.
[19] 王鑫. 虾池微囊藻毒素变化规律及其对南美白对虾毒性作用机制研究[D]. 杭州:中国计量大学,2018.
[20] 王志铮,申屠琰,熊威. 4种消毒剂对麦瑞加拉鲮鱼幼鱼的急性毒性研究[J]. 海洋水产研究,2007,28(3):92-97.
[21] LI X Y,CHUNG I K,KIM J I,et al.Subchronic oral toxicity of microcystin in common carp(Cyprinus carpio L.) exposed to Microcystis under laboratory conditions[J]. Toxicon:Official Journal of the International Society on Toxinology,2004,44(8):821-827.
[22] MOHAMED Z A.Accumulation of cyanobacterial hepatotoxins by Daphnia in some Egyptian irrigation canals[J]. Ecotoxicology Environment Safety,2001,50(1):4-8.
[23] CHEN J,XIE P.Tissue distributions and seasonal dynamics of the hepatotoxic microcystins-LR and -RR in two freshwater shrimps,Palaemon modestus and Macrobrachium nipponensis,from a large shallow,eutrophic lake of the subtropical China[J]. Toxicon,2005,45(5):615-625.
[24] GARCIA A C,BARGU S.Evaluating the potential risk of microcystins to blue crab(Callinectes sapidus)fisheries and human health in a eutrophic estuary[J]. Harmful Algae,2010,9(2):134-143.
[25] 沈强,刘永定,李敦海,等. 白鲢罗非鱼对微囊藻毒素急性、亚急性毒性响应[J]. 中国环境科学,2019,39(6):2633-2643.
[26] RUEBHART D R,WICKRAMASINGHE W,COCK I E.Protective efficacy of the antioxidants vitamin E and trolox against Microcystis aeruginosa and microcystin-LR in Artemia franciscana nauplii[J]. Journal of Toxicology & Environmental Health,2009,72(24):1567-1575.
[27] 刘永叶,王宇飞,乔亚华,等. 我国典型核电厂址海域中广域性鱼类的热耐受性研究[J]. 海洋环境科学,2019,38(4):528-532.
[28] 袁瑞鹏,刘建勇,张嘉晨,等. 凡纳滨对虾群体杂交与自交F₁低溶氧与高氨氮耐受性比较[J]. 中国水产科学,2015,22(3):410-417.
[29] 傅一鸣,李智,柳峰松,等. 微囊藻毒素MC-LR对凡纳滨对虾细胞免疫相关基因表达水平的影响[J]. 上海海洋大学学报,2015,24(2):196-202.
[30] 刘祖毅,王志铮,吕敢堂,等. 3种有机磷农药对黑鲷幼鱼的急性毒性研究[J]. 浙江海洋学院学报(自然科学版),2010,29(1):20-24.
[31] 徐海滨,隋海霞,高士荣,等. 微囊藻毒素在鲤鱼体内生物富集作用的初步研究[J]. 中国食品卫生杂志,2003,15(3):202-204.
[32] 李旭光,周刚,周军,等. 太湖微囊藻毒素在罗非鱼体内累积及生物降解的初步研究[J]. 水生态学杂志,2010,3(1):67-70.
[33] PRIETO A I,PICHARDO S,JOS A,et al.Time-dependent oxidative stress responses after acute exposure to toxic cyanobacterial cells containing microcystins in tilapia fish(Oreochromis niloticus)under laboratory conditions[J]. Aquatic Toxicology,2007,84(3):337-345.

Study on acute toxicity of microcystins to Litopenaeus vannamei

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