Abstract: To screen tomato germplasm resources with high resistance to chlorosis virus（ToCV）, 243 tomato germplasm resources were collected and screened through seed quality, growth vigor, disease resistance, fruit yield and quality. First, the collected 254 germplasm resources were screened through germination rate. Germplasm resources with a germination rate lower than 80% and a germination energy lower than 60% were removed, and 132 germplasm resources were obtained for subsequent research. Thereafter, a comprehensive ranking of growth vigor was conducted through principal component analysis, and the top 20 germplasm resources in the ranking were selected for disease resistance analysis. In the disease resistance determination of chlorosis virus disease, the disease indices of ‘Ls-693’ and ‘2023-20-76’ were 34.4 and 37.5 respectively, both exhibiting high resistance（HR）. Moreover, no plants of ‘Ls-693’ showed disease severity grades 3 and 4, indicating higher disease resistance. In terms of fruit nutritional indicators, the soluble sugar content of ‘Ls-693’ fruit was 3.67%, which was at a relatively high level, with a balanced sweet and sour taste and good flavor, and the lycopene content reached 14.96 mg/100g. The soluble protein content of ‘2023-20-76’ was 3.10 mg/g, and the vitamin C content of both was approximately 16 mg/100g, which was at a relatively high level. Based on the above indicators, ‘Ls-693’ and ‘2023-20-76’ showed the best overall performance. The two tomato germplasm resources screened in this experiment have both excellent phenotypic characteristics and strong disease resistance, and the experimental results provide an important material basis for subsequent tomato disease-resistant breeding.

Key words: tomato germplasm resources, screening, disease resistance, fruit quality