Journal of Tianjin Agricultural University ›› 2025, Vol. 32 ›› Issue (1): 19-24.doi: 10.19640/j.cnki.jtau.2025.01.004

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Study on the alleviation of phytotoxicity of clopyralid by sodium nitrophenolate to oilseed rape

Wang Fuhao1, Guo Jinghua1, Hao Peipei2, Cheng Youpu1,Corresponding Author   

  1. 1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China;
    2. Tianjin Lüheng Chemical Co., Ltd., Tianjin 300270, China
  • Received:2023-11-20 Published:2025-03-04

Abstract: In order to explore the mechanism of sodium nitrophenolate alleviating the phytotoxicity of clopyralid on rape, an indoor pot experiment was carried out. When the three leaves of rape seedlings were one heart, one group was sprayed with 0(CK), 0.4, 0.8(recommended dose), 1.6, 3.2, 4.0 g/L clopyralid, and the other group was sprayed with 9 mg/L sodium nitrophenolate on the leaf surface 24 h after treatment. The changes of antioxidant enzyme activity, chlorophyll and carotenoid content in the leaves of the two groups were compared.The results showed that the activities of antioxidant enzymes(CAT, POD, SOD)and the content of reduced glutathione(GSH)in rape seedlings increased first and then decreased with the increase of mass concentration of clopyralid. When the mass concentration of clopyralid was 1.6 g/L, the activity of CAT was the highest. When the mass concentration was 3.2 g/L, the increase of CAT activity decreased, the contents of chlorophyll and carotenoid decreased by 30.2 % and 6.0% respectively compared with CK, and the content of malondialdehyde(MDA)increased by 165.6%, when the mass concentration was 4.0 g/L. POD, SOD activity and GSH content were 11.0%, 26.0% and 17.7% lower than CK, MDA content increased by 195.5%, chlorophyll and carotenoid content decreased by 62.9% and 23.7%, respectively.After spraying sodium nitrophenolate, the activities of CAT, POD, SOD and the contents of GSH, chlorophyll and carotenoid in rape leaves were significantly increased compared with those treated with the same concentration of clopyralid. Compared with those before remission, MDA content decreased by 8.7%-44.5%, POD activity increased by 7.0%-11.8%, SOD activity increased by 10.1%-68.7%, GSH content increased by 8.5%-19.6%, chlorophyll content increased by 7.0%-28.9%, and carotenoid content increased by 7.1% -62.0%. In summary, low mass concentration of clopyralid can increase the antioxidant enzyme activity and GSH content of rape leaves, thereby improving the stress resistance of rape. When the mass concentration of clopyralid is too high, the MDA content of rape leaves is significantly increased, and the antioxidant enzyme activity, GSH content, chlorophyll content, and carotenoid content are significantly inhibited. After spraying sodium nitrophenolate, the inhibition effect is alleviated to varying degrees. With the increase of the mass concentration of clopyralid, the mitigation effect is weakened, indicating that sodium nitrophenolate can alleviate the phytotoxicity of clopyralid to rape to a certain extent.

Key words: clopyralid, sodium nitrophenolate, rape, relieving effect

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