牛ACOT11基因克隆及其编码蛋白生物信息学分析

Journal of Tianjin Agricultural University ›› 2015, Vol. 22 ›› Issue (3): 1-6.

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Cloning and Encoding Protein Bioinformatics Analysis of Bovine ACOT11 Gene

HU Xiao-wei, JIN Cong-fei, LIU Xin-feng, GUO Hong^*

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2015-05-06 Published:2015-09-30

Abstract

Abstract: In this study, bovine ACOT11 was cloned, and encoding protein was analyzed by bioinformatics methods. The human ACOT11 gene sequence in GenBank was chosen as probe to BLAST and the expressed sequence tags (ESTs) of bovine ACOT11 gene were gotten. The bovine ACOT11 gene was amplified by sequencing assembling of ESTs and cDNA sequence, which was amplified by reverse transcription polymerase chain reaction (RT?PCR). Sequence analyses show that the DNA region of cattle ACOT11 gene spans approximately 4.9 kb and the homology of mRNA between cattle and human ACOT11 is 87%. The bovine ACOT11 gene contains 16 exons and 15 introns, whose coding region is 1 620 bp and 539 amino acids were encoded. The secondary structure prediction of ACOT11 protein is mainly based on alpha helix, and ACOT11 protein contains 35 phosphorylation sites and one START domain, the phosphorylation sites locate in serine (Ser), threonine (Thr) and tyrosine (Tys) residue, the subcellular localization of ACOT11 is predicted in the nucleus, which didn’t belong to the secreted protein.

Key words: bovine, ACOT11 gene, cloning, bioinformatics analysis, protein structure

CLC Number:

S823

HU Xiao-wei, JIN Cong-fei, LIU Xin-feng, GUO Hong. Cloning and Encoding Protein Bioinformatics Analysis of Bovine ACOT11 Gene[J]. Journal of Tianjin Agricultural University, 2015, 22(3): 1-6.

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Cloning and Encoding Protein Bioinformatics Analysis of Bovine ACOT11 Gene

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