牛骨骼肌卫星细胞中miR-143互作lncRNA-HZ5的鉴定及其相互调控作用分析

doi:10.19640/j.cnki.jtau.2019.03.012

Journal of Tianjin Agricultural University ›› 2019, Vol. 26 ›› Issue (3): 51-56.doi: 10.19640/j.cnki.jtau.2019.03.012

• Researches and Scientific Notes • Previous Articles Next Articles

The analysis of the interaction relationship between miR-143 and lncRNA-HZ5 in bovine skeletal muscle satellite cells

WANG Yi-min, ZHANG Wei-ran, ZHANG Jun-xing, LIU Xin-feng, GUO Hong, DING Xiang-bin

Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-06-18 Online:2019-09-30 Published:2019-09-30

Abstract

Abstract: In order to explore the relationship between miR-143 and its interacted lncRNA and its mechanism in the process of bovine myogenic differentiation, two predicted highly expressed（lncRNA lncRNA-HZ5 and lncRNA-HZ8）were chosen for further research. The relationship between miR-143 and predicted interacted lncRNA were identified by dual-luciferase assay and qRT-PCR detection, the influence of lncRNA on the protein level of miR-143’s target gene was detected by western blot. The results showed that lncRNA-HZ5 was directly regulated by miR-143 using dual-luciferase assay. The expression of lncRNA-HZ5 was significantly down-regulated when miR-143 was over expressed, and significantly up-regulated when miR-143 was inhibited. It was found that inhibition of lncRNA-HZ5 expression using siRNA could down-regulate miR-143 and up-regulate the protein level of miR-143’s target gene IGFBP5. The results showed that the miR-143 could interact with lncRNA-HZ5 in bovine skeletal muscle satellite cells, which indicated that miR-143 may regulate the bovine myogenic differentiation by interacting with lncRNA-HZ5.

Key words: miR-143, lncRNA-HZ5, bovine, skeletal muscle satellite cells, myogenic differentiation

CLC Number:

S814.8

WANG Yi-min, ZHANG Wei-ran, ZHANG Jun-xing, LIU Xin-feng, GUO Hong, DING Xiang-bin. The analysis of the interaction relationship between miR-143 and lncRNA-HZ5 in bovine skeletal muscle satellite cells[J]. Journal of Tianjin Agricultural University, 2019, 26(3): 51-56.

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The analysis of the interaction relationship between miR-143 and lncRNA-HZ5 in bovine skeletal muscle satellite cells

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