Abstract: Different concentrations (0, 5, 10, 15 g·L^-1) of sucrose and different concentrations (0, 0.2, 0.4, 0.6, 0.8 mg·L^-1）of IBA were respectively added to media supported by soil, and the stem section rooting of the Deutzia hybrida ‘Boule’ plantlet in Vitro, acclimatizing in opened culture bottle, transplanting with lump and the stoma closing state of the plantlet during acclimatized in opened culture bottles were studied. The results show as follows. In the sterile soil without sucrose and IBA added, the rooting rate of the Deutzia hybrida ‘Boule’ plantlet was 100%, and the root length and the stem and leaf growth of the plantlet were not significantly different from those of the plantlet in medium containing sucrose or IBA. Under the transplanting conditions without plastic film covering or spraying and the air humidity decreased to 40% in afternoon, the survival rate of the plantlet transplanted with lump was 100%, which was obvious higher than that of the plantlet transplanted with normal transplanting method (72.2%). After acclimatized in opened culture bottles for 14 days, the half closing rate of the stoma in leaf of the plantlet cultured in soil supporting medium in dark increased from 1.3% in no opened culture bottles to 98.7%, while the stoma relative opening degree decreased from 0.618 in no opened culture bottles to 0.193. The results provide the basis for improving the rapid micropropagation system of Deatzia hybrida ‘Boule’.

Key words: Deutzia hybrida ‘Boule’, plantlet in Vitro, soil supporting, rooting, transplanting with lump