苯酚对红鳍东方鲀肝胰脏CuZn-SOD和Mn-SOD基因表达的半定量分析

Journal of Tianjin Agricultural University ›› 2014, Vol. 21 ›› Issue (3): 1-6.

• Researches and Scientific Notes • Next Articles

Expression Analysis of CuZn-SOD and Mn-SOD Genes in Hepatopancreas of Takifugu rubripes after Phenol Exposure

SHI Hong-yue¹, CUI Pei¹, JIANG Zhi-qiang^{2,Corresponding Author}, CHEN Cheng-xun¹

1.Tianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
2. Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, Liaoning Province, China

Received:2014-06-30 Online:2014-09-20 Published:2019-10-21

Abstract

Abstract: The semi-quantitative RT-PCR method was applied to test the expression of CuZn-SOD gene and Mn-SOD gene in Takifugu rubripes hepatopancreas after phenol exposure. Fish was cultured in seawater containing 0, 0.18, 0.32, 0.56, 1.00 and 1.35 mg/L phenol, respectively, and was sampled before phenol exposure and after 4, 8, 12 and 16 d phenol exposure. The results show that basal expression of CuZn-SOD gene and Mn-SOD gene were detected in fish before phenol exposure. The relative expression levels of CuZn-SOD and Mn-SOD mRNA showed a trend of gradual up-regulation throughout the 16 days phenol exposure and the phenol induced the expression of CuZn-SOD and Mn-SOD gene. The mRNA expression level in each phenol exposure group peaked at the fourth day and was significantly higher than that of the phenol pre-exposure level（P<0.05）.

Key words: phenol, Takifugu rubripes, toxicity test, CuZn-SOD, Mn-SOD

CLC Number:

S941.91

SHI Hong-yue, CUI Pei, JIANG Zhi-qiang, CHEN Cheng-xun. Expression Analysis of CuZn-SOD and Mn-SOD Genes in Hepatopancreas of Takifugu rubripes after Phenol Exposure[J]. Journal of Tianjin Agricultural University, 2014, 21(3): 1-6.

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Expression Analysis of CuZn-SOD and Mn-SOD Genes in Hepatopancreas of Takifugu rubripes after Phenol Exposure

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