花鲈弗氏柠檬酸杆菌的分离与鉴定

doi:10.19640/j.cnki.jtau.2026.02.011

Journal of Tianjin Agricultural University ›› 2026, Vol. 33 ›› Issue (2): 63-68.doi: 10.19640/j.cnki.jtau.2026.02.011

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Isolation and identification of Citrobacter freundii from Lateolabrax maculatus

Li Chunxiao, Li Xiuyi, Han Zhuoran, Luo Zhang, Sun Jingfeng^{Corresponding Author}

College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2024-09-12 Online:2026-04-30 Published:2026-05-06

Abstract

Abstract: In order to determine the cause of the disease of Lateolabrax maculatus, the dominant strains were isolated from the liver, spleen and kidney tissues. The species were identified by morphological observation, physiological and biochemical tests, 16S rRNA and gyrB gene sequencing and phylogenetic analysis. The pathogenicity was analyzed by artificial regression infection test, and the resistance to antibiotics was analyzed by drug sensitivity test. The results showed that a dominant strain was isolated from the liver of diseased fish and identified as Citrobacter freundii, named XY-1. The artificial regression infection test confirmed that strain XY-1 was the pathogen causing the disease of Lateolabrax maculatus, and the median lethal dose LD₅₀ of Lateolabrax maculatus was 1.89×10⁶ CFU/mL. The results of drug sensitivity test showed that strain XY-1 was highly sensitive to 9 drugs such as cefazolin, gentamicin and florfenicol, moderately sensitive to 3 drugs such as neomycin, rifampicin and norfloxacin, and resistant to 12 drugs such as amoxicillin, ampicillin and cefradine. The results of this study can provide a reference for the diagnosis and prevention of bacterial diseases caused by Citrobacter freundii in Lateolabrax maculatus.

Key words: Citrobacter freundii, 16S rRNA, gyrB, Lateolabrax maculatus, isolation and identification

CLC Number:

S943

Li Chunxiao, Li Xiuyi, Han Zhuoran, Luo Zhang, Sun Jingfeng. Isolation and identification of Citrobacter freundii from Lateolabrax maculatus[J]. Journal of Tianjin Agricultural University, 2026, 33(2): 63-68.

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References

[1] 潘春华. 鲈鱼之美[J]. 中国水产,2024(7):74-75.
[2] 姜森颢,雷雨婷,刘畅,等. 我国花鲈综合养殖模式研究进展[J]. 现代农业研究,2024,30(4):78-80.
[3] 林星. 无公害花鲈淡水健康养殖技术[J]. 水产养殖,2012,33(2):27-29.
[4] 王甘翔,沈佳健,黄福勇,等. 陆基生态循环系统养殖花鲈和梭鱼新技术[J]. 水产养殖,2021,42(7):66,69.
[5] 温海深,张美昭,李吉方,等. 我国花鲈养殖产业现状与种子工程研究进展[J]. 渔业信息与战略,2016,31(2):105-111.
[6] 温海深,李昀,张美昭,等. 花鲈——家喻户晓的中国海鲈[J]. 中国水产,2020(8):110-111.
[7] 葛婉仪,雷丽娜,蒋昕彧,等. 花鲈b2m基因cDNA的克隆及表达分析[J]. 南方水产科学,2020,16(6):47-56.
[8] HU J M,WANG B T,FENG J,et al.Edwardsiella piscicida,a pathogenic bacterium newly detected in spotted sea bass Lateolabrax maculatus in China[J]. Aquaculture Reports,2022,22:100973.
[9] 林能锋,潘滢,许斌福,等. 养殖花鲈(Lateolabrax maculatus)肠道菌群的多样性分析[J]. 水产养殖,2021,42(6):1-7.
[10] 乔丹,丁斐斐,李长红,等. 花鲈结合珠蛋白基因的克隆及其在哈维氏弧菌感染下的表达分析[J]. 农业生物技术学报,2019,27(11):2042-2051.
[11] GUO H W,ZHANG B,WANG P F,et al.Spotted sea bass(Lateolabrax maculatus)NOD2 gene involved in the immune response against Vibrio harveyi infection[J]. Journal of Fish Diseases,2023,46(8):1-12.
[12] 王宝屯,颜远义,毛灿,等. 82种中草药及其复方对花鲈源维氏气单胞菌的体外抑菌效果[J]. 华南师范大学学报(自然科学版),2021,53(3):64-72.
[13] 肖双燕,韦冬冬,刘明珠,等. 广西地区美国牛蛙源弗氏柠檬酸杆菌的分离鉴定、耐药性及组织病理[J]. 水产学报,2024,48(5):118-130.
[14] 薛巧,赵战勤,刘会胜,等. 弗氏柠檬酸杆菌对动物和人致病性研究进展[J]. 动物医学进展,2015,36(7):81-85.
[15] 谢海平,喻佳俊,黄清,等. 基于MALDI-TOF MS技术对加州鲈病原弗氏柠檬酸杆菌的快速鉴定[J]. 淡水渔业,2020,50(6):40-45.
[16] 刘亚暖,姜肇旭,颜世敢. 弗氏柠檬酸杆菌的致病性及分型研究进展[J]. 工业微生物,2023,53(4):53-56.
[17] 徐静,冯永其,段俊堂,等. 1株灰鹦鹉源弗氏柠檬酸杆菌的分离鉴定及系统发育分析[J]. 中国兽医杂志,2020,56(12):60-61.
[18] CARDOSO P H M,MORENO L Z,DE OLIVEIRA C H,et al. Main bacterial species causing clinical disease in ornamental freshwater fish in Brazil[J]. Folia Microbiologica,2020,66(2):231-239.
[19] 刘张淮,吴霆,王家军,等. 克氏原螯虾烂尾病病原的分离鉴定及其相关特性分析[J]. 水产科学,2022,41(1):102-109.
[20] 裴巧林,陈德芳,张小丽,等. 小龙虾柠檬酸杆菌感染诊断分析[J]. 科学养鱼,2023(1):46-48.
[21] 石征宇,易弋,黄德生,等. 一株弗氏柠檬酸杆菌的分离鉴定及致病性研究[J]. 中国畜牧兽医,2018,45(2):479-485.
[22] 杨方园,关瑞章,李忠琴,等. 花鳗鲡病原菌弗氏柠檬酸杆菌的鉴定[J]. 集美大学学报(自然科学版),2013,18(2):81-87.
[23] 张冬星,康元环,田佳鑫,等. 团头鲂致病性弗氏柠檬酸杆菌的分离鉴定及药敏试验[J]. 中国兽医科学,2016,46(12):1589-1595.
[24] DENG D,MU Z Z,LV X Y,et al.Pathogenicity of Plesiomonas shigelloides and Citrobacter freundii isolated from the endangered Chinese sturgeon(Acipenser sinensis)[J]. Microbial Pathogenesis,2022,173:105818.
[25] ALICIA I,ALEJANDRO S,PASCUAL C,et al.Citrobacter freundii-associated lesions in stranded loggerhead sea turtles (Caretta caretta)[J]. Veterinary Pathology,2023,61(1):140-144.
[26] 东秀珠,蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社,2001.
[27] R.E.布坎南. 伯杰细菌鉴定手册[M]. 9版. 北京:科学出版社,1995.
[28] 霍佳昕,胡秀彩,夏苏东,等. 小丑鱼创伤弧菌的分离鉴定及药敏特性研究[J]. 天津农学院学报,2024,31(4):49-54.
[29] 黄亚明,李长乐,覃诗君,等. 红螯螯虾源布氏柠檬酸杆菌分离鉴定和耐药性分析[J]. 中国兽医杂志,2024,60(1):59-65.
[30] XIONG F,QIN L,HAO Y T,et al.Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp(Ctenopharyngodon idellus)[J]. Aquaculture,2020,521:735015.
[31] ZOU S,GONG L,TAHIR A K,et al.Comparative analysis and gut bacterial community assemblages of grass carp and crucian carp in new lineages from the Dongting Lake area[J]. Microbiology Open,2020,9(5):996.
[32] SUN H Y,CAO X H,JIANG Y F,et al.Outbreak of a novel disease associated with Citrobacter freundii infection in freshwater cultured stingray,Potamotrygon motoro[J]. Aquaculture,2018,492:35-39.
[33] 郑善坚. 弗氏柠檬酸杆菌引起鱼、蛙溃烂病的防治案例[J]. 科学养鱼,2017(2):68-69.
[34] 宝小雪,刘华,余莹英,等. 牛蛙源迟缓爱德华氏菌和弗氏柠檬酸杆菌的分离鉴定及药敏试验[J]. 黑龙江畜牧兽医,2022(1):122-125,129,139.
[35] 田飞焱,欧阳敏,张晓燕,等. 中华鳖弗氏柠檬酸杆菌的分离鉴定及药物敏感性试验[J]. 水产学杂志,2013,26(4):42-46.
[36] SVETLANA J,DOBRILA J D,LJ V.Citrobacter freundii as a cause of disease in fish[J]. Acta Veterinarian,2003,53(5/6):399-410.
[37] 唐伟林,伍志勇. 水产养殖中常见鱼病的防治方法[J]. 水产养殖,2021,42(4):67-69.
[38] 罗伟杰,史磊,黄瑜,等. 大口黑鲈源豚鼠气单胞菌的分离、鉴定及病理性分析[J]. 广东农业科学,2024,51(7):66-78.
[39] 何海龙,王云山,李正伟,等. 水产养殖用抗生素使用注意事项[J]. 黑龙江水产,2022,41(4):63-64.
[40] 浦岩松. 抗生素在水产养殖应用存在的问题及对策[J]. 黑龙江水产,2023,42(4):291-293.
[41] 张明洋,胡安东,程振涛,等. 虹鳟弗氏柠檬酸杆菌的分离鉴定与多位点序列分型[J]. 基因组学与应用生物学,2020,39(6):2521-2528.
[42] 黄志艺,谢丽琴,许嘉龙,等. 牛蛙源弗氏柠檬酸杆菌的分离鉴定和药敏分析[J]. 韩山师范学院学报,2022,43(6):45-51,73.
[43] 苏英,吴荣华,申君宇,等. 大鲵源致病性弗氏柠檬酸杆菌的分离、鉴定和药物敏感性[J]. 淡水渔业,2019,49(4):56-61.
[44] 陈绮梨,常藕琴,张德峰,等. 加州鲈源弗氏柠檬酸杆菌的分离鉴定及耐药性分析[J]. 南方农业学报,2021,52(2):465-474.
[45] 陈红莲,宋光同,何吉祥,等. 克氏原螯虾弗氏柠檬酸杆菌的分离鉴定与药敏试验[J]. 淡水渔业,2014,44(1):73-77.
[46] 马亮,董靖,张国栋,等. 克氏原螯虾源普通变形杆菌分离、鉴定及联合药敏试验[J]. 淡水渔业,2022,52(5):53-59.
[47] 彭小倩,任朝颖,邓雪玥,等. 杂交鲟源温和气单胞菌的分离鉴定及生物学特性的研究[J]. 淡水渔业,2023,53(3):62-70.
[48] 藏淑梅. 水产养殖用抗生素的应用及注意事项[J]. 黑龙江水产,2023,42(5):407-408.

Isolation and identification of Citrobacter freundii from Lateolabrax maculatus

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