一株源自蛋鸡的沃氏葡萄球菌分离鉴定与耐药性分析

doi:10.19640/j.cnki.jtau.2025.01.008

Journal of Tianjin Agricultural University ›› 2025, Vol. 32 ›› Issue (1): 39-43.doi: 10.19640/j.cnki.jtau.2025.01.008

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Isolation, identification and drug resistance analysis of a strain of Staphylococcus warneri from laying hens

Ran Hengdong, Wu Dingzhou, Wang Yongzhen, Liu Yanfei^{Corresponding Author}, Yang Jiande^{Corresponding Author}

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China

Received:2022-11-15 Published:2025-03-04

Abstract

Abstract: Staphylococcus warneri is a coagulase negative Staphylococcus. In recent years, the incidence rate of infection caused by this pathogen has gradually increased and showed multiple drug resistance. In this study, the bacteria were isolated from the purulent lesion of adult laying hens from a chicken farm in Tianjin, and the isolated strains were identified by staining microscopy, biochemical test, drug sensitivity test and 16S rRNA gene sequencing. The homology of the sequencing results was analyzed and a phylogenetic tree was constructed. The results showed that gram-positive cocci were detected by staining microscopy, and the biochemical and culture results were consistent with the characteristics of Staphylococcus. The 16S rRNA gene sequence analysis showed a high homology of 99.8% with the warning type Staphylococcus, and the isolated strain was determined to be Staphylococcus warneri. The isolated bacteria were sensitive to tetracycline, minocycline, ceftriaxone, cefquinoxime, norfloxacin, ciprofloxacin, levofloxacin, clindamycin and vancomycin, and resistant to penicillin G, erythromycin and clarithromycin. The results of this study can provide reference for the prevention, control and treatment of infection caused by Staphylococcus warneri.

Key words: Staphylococcus warneri, biochemical test, drug sensitivity test, 16S rRNA, phylogenetic tree, drug resistance

CLC Number:

S852.61

Ran Hengdong, Wu Dingzhou, Wang Yongzhen, Liu Yanfei, Yang Jiande. Isolation, identification and drug resistance analysis of a strain of Staphylococcus warneri from laying hens[J]. Journal of Tianjin Agricultural University, 2025, 32(1): 39-43.

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Isolation, identification and drug resistance analysis of a strain of Staphylococcus warneri from laying hens

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