犬脂肪间充质干细胞静脉移植临床安全性研究

doi:10.19640/j.cnki.jtau.2023.06.010

Journal of Tianjin Agricultural University ›› 2023, Vol. 30 ›› Issue (6): 62-68.doi: 10.19640/j.cnki.jtau.2023.06.010

• Researches and Scientific Notes • Previous Articles Next Articles

Clinical safety of canine adipose derived mesenchymal stem cells in vein transplantation

Luo Jiaming¹, Ge Huaina², Bai Chunna², Ge Xiuguo^{1, Corresponding Author}

1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China;
2. Baioupai Tianjin Biotechnology Co., Ltd., Tianjin 300350, China

Received:2023-01-01 Online:2023-12-31 Published:2024-02-05

Abstract

Abstract: In order to verify the safety of clinical application of canine adipose derived stem cells （ASCs）, the fourth generation of canine ASCs were selected in this experiment, and the cell morphology was observed under the microscope, and adipogenesis, osteogenesis and chondrogenesis were induced and differentiated, and cell surface marker proteins were analyzed. The results showed that ASCs grew as spindle-like adherent cells with uniform morphology. ASCs could differentiate into adipocytes, osteocytes and chondrocytes in vitro. Canine ASCs express marker proteins CD29, CD44, CD105 and CD166, but do not express CD34 and CD45, which conforms to the typical characteristics of adipose mesenchymal stem cells. Canine ASCs with 1×10⁶/kg vein transplantation was performed at a speed of 10 mL/（kg·h）. No adverse reaction was found during the injection. Long term observation for 3 months showed no obvious complications. Compared with that before transplantation, the number of white blood cells, neutrophils, monocytes, eosinophils, red blood cells, hemoglobin and platelets showed no significant difference（P>0.05）; Serum biochemical examination showed that there were no significant differences in liver indicators such as albumin, ALT, AKP（P>0.05）, renal indicators such as creatinine, serum urea, blood phosphorus and blood calcium（P>0.05）, pancreatic related indicators such as blood glucose and serum amylase（P>0.05）, myocardial injury indicators such as creatine kinase and blood lipid indicators such as cholesterol before and after cell transplantation（P>0.05）. The results of this study showed the clinical safety of ASCs in dogs and laid a foundation for cell transplantation therapy.

Key words: canine, adipose mesenchymal stem cells, transplantation, safety

CLC Number:

S854.5
Q28

Luo Jiaming, Ge Huaina, Bai Chunna, Ge Xiuguo. Clinical safety of canine adipose derived mesenchymal stem cells in vein transplantation[J]. Journal of Tianjin Agricultural University, 2023, 30(6): 62-68.

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Clinical safety of canine adipose derived mesenchymal stem cells in vein transplantation

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