Abstract: In this paper, with the stolon tip of ‘Xiangye’ strawberry used as test material, the key technology of rapid propagation of explants such as sterilization, induction and differentiation culture, subculture and rooting culture were studied with tissue culture method, and the rapid propagation technology of strawberry cultivar ‘Xiangye’ stem tip tissue culture was established. The results showed that using 10% sodium hypochlorite to disinfect the explants for 5 minutes, the disinfection effect was the best and the survival rate was the highest, reaching 64.44%. The best medium for strawberry explant induction and differentiation was MS+Thidiazuron（TDZ）1.0 mg/L+Naphthalene acetic acid（NAA）0.1 mg/L, and the induction and differentiation rate was 67.78% at the stage of explant induction and differentiation. The best medium formula for cluster bud multiplication of strawberry was MS+TDZ（0.75 mg/L）+NAA（0.2 mg/L）, and the value-added coefficient was 4.11 at the stage of cluster bud subculture and multiplication. The optimum medium for rooting of strawberry cluster buds was MS+NAA 0.1 mg/L and the rooting rate was 98.33% in the rooting stage.

Key words: strawberry, shoot tip, disinfection, hormone, tissue culture