天津农学院学报 ›› 2026, Vol. 33 ›› Issue (5): 26-31.doi: 10.19640/j.cnki.jtau.2026.05.004

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脯氨酸浸种对盐胁迫下冬小麦种子活力和幼苗有机渗透调节物质含量的影响

杜锦1, 王志强2, 姜立3, 曹高燚1,3,通信作者   

  1. 1.天津农学院 农学与资源环境学院,天津 300392;
    2.天津市宝坻区农业发展服务中心,天津 301800;
    3.张家川回族自治县农业技术推广站,甘肃 天水 741500
  • 收稿日期:2025-03-20 发布日期:2026-11-02
  • 通讯作者: 曹高燚(1984—),男,副教授,博士,主要从事种子科学与分子育种方面的研究。E-mail:caogaoyi@tjau.edu.cn。
  • 作者简介:杜锦(1984—),男,高级实验师,硕士,主要从事鲜食玉米遗传育种与种子抗逆机理的研究。E-mail:401558171@qq.com。
  • 基金资助:
    天津市科技帮扶项目(25ZXBTSN00030); 甘肃省科技厅创新引导计划东西部科技协作专项(26CXNE010)

Effects of proline seed soaking on seed vigor and seedling organic osmolyte contents of winter wheat under salt stress

Du Jin1, Wang Zhiqiang2, Jiang Li3, Cao Gaoyi1,3,Corresponding Author   

  1. 1. College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300392, China;
    2. Baodi District Agricultural Development Service Center, Tianjin 301800, China;
    3. Zhangjiachuan Hui Autonomous County Agricultural Technology Exten
  • Received:2025-03-20 Published:2026-11-02

摘要: 充分利用盐渍化土壤是提高粮食总产量的重要途径之一。研究利用外源物质提高冬小麦耐盐性具有重要意义。本研究分析了不同浓度脯氨酸浸种对盐胁迫下冬小麦种子活力及幼苗中有机渗透调节物质含量的影响,结果表明:250 μmol/L脯氨酸浸种可显著提高50 mmol/L NaCl胁迫下冬小麦种子的发芽势、发芽率、发芽指数、活力指数以及苗高和根长,并提高90 mmol/L NaCl胁迫下幼苗中脯氨酸、可溶性糖和可溶性蛋白含量;350 μmol/L 脯氨酸浸种在50 mmol/L NaCl胁迫下仅显著提高发芽指数;450 μmol/L脯氨酸浸种对部分萌发和生长指标表现出抑制作用。综合来看,250 μmol/L脯氨酸浸种对低盐胁迫下冬小麦种子萌发和幼苗生长具有较好的促进作用,可为盐渍化土壤条件下冬小麦播前种子处理提供参考。

关键词: 冬小麦, 盐胁迫, 脯氨酸, 种子活力, 有机渗透调节物质

Abstract: Since making full use of salinized soil is one of the important ways to increase grain output, it is of great significance to study exogenous substances to improve salt tolerance of winter wheat. In this study, the effects of proline seed soaking at different concentrations on seed vigor and organic osmolyte contents in seedlings of winter wheat under salt stress were investigated. The results showed that seed soaking with 250 μmol/L proline significantly increased germination energy, germination percentage, germination index, vigor index, seedling height, and root length under 50 mmol/L NaCl stress. Under 90 mmol/L NaCl stress, proline soaking increased the contents of proline, soluble sugar, and soluble protein in winter wheat seedlings, with the 250 μmol/L treatment showing the overall greatest effect. Seed soaking with 350 μmol/L proline significantly increased only the germination index under 50 mmol/L NaCl stress, whereas 450 μmol/L proline showed inhibitory effects on some germination and growth traits. These results indicate that an appropriate concentration of proline, particularly 250 μmol/L, can improve seed germination and seedling growth of winter wheat under low salt stress.

Key words: winter wheat, salt stress, proline, seed vigor, organic osmolyte

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