急性低盐胁迫对黄鳍金枪鱼幼鱼渗透调节影响研究

doi:10.19640/j.cnki.jtau.2024.04.011

天津农学院学报 ›› 2024, Vol. 31 ›› Issue (4): 55-65.doi: 10.19640/j.cnki.jtau.2024.04.011

急性低盐胁迫对黄鳍金枪鱼幼鱼渗透调节影响研究

张宁璐^1,2,4, 周胜杰^1,2,3,4, 陈成勋¹, 于刚^2,3,4, 马振华^{1,2,3,4,通信作者}, 孙金辉^{1,通信作者}

1.天津农学院水产学院,天津 300392;
2.三亚热带水产研究院,海南三亚 572018;
3.海南省深远海渔业资源高效利用与加工重点实验室,海南三亚 572018;
4.中国水产科学研究院南海水产研究所热带水产研究开发中心,海南陵水 572400

收稿日期:2023-09-28 出版日期:2024-08-31 发布日期:2024-09-11
通讯作者: 马振华（1981—）,男,研究员,博士,主要从事海水鱼类繁育与深远海养殖研究工作。E-mail：zhenhua.ma@hotmail.com。孙金辉（1979—）,男,教授,博士,主要从事水生动物行为生态学研究工作。E-mail：Jhsun1008@163.com。
作者简介:张宁璐（1996—）,女,硕士在读,主要从事海水鱼类繁育与养殖研究工作。E-mail：305419063@qq.com。
基金资助:
海南省重大项目资助（ZDK2021011）; 三亚崖州湾科技城科技专项资助（SKJC-2022-PTDX-015）; 中国水产科学研究院基本科研业务费专项（2020TD55,2020XT0301）; 中国-东盟现代海洋渔业技术合作及产业化开发示范（无编号）; 中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助（2021SD09）; 广西创新驱动发展专项（桂科AA18242031）

Effects of acute low salt stress on osmotic regulation of juvenile yellowfin tuna（Thunnus albacares）

Zhang Ninglu^1,2,4, Zhou Shengjie^1,2,3,4, Chen Chengxun¹, Yu Gang^2,3,4, Ma Zhenhua^{1,2,3,4,Corresponding Author}, Sun Jinhui^{1,Corresponding Author}

1. College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China;
2. Sanya Tropical Fisheries Research Institute, Sanya 572018, Hainan Province, China;
3. Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya 572018, Hainan Province, China;
4. Tropical Fisheries Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, Hainan Province, China

Received:2023-09-28 Online:2024-08-31 Published:2024-09-11

摘要/Abstract

摘要： 为了研究急性低盐胁迫对黄鳍金枪鱼幼鱼渗透调节的影响,将黄鳍金枪鱼幼鱼由盐度34转入盐度29的低盐海水（试验组）和盐度34的海水（对照组）中,并在转移后0、6、24、48 h采样,分析急性低盐胁迫对黄鳍金枪鱼幼鱼血清渗透压、催乳素（PRL）、乳酸、血糖与离子浓度的影响。研究结果显示,渗透压、血清乳酸、血糖、钠离子（Na⁺）、钾离子（K⁺）、氯离子（Cl^–）、钙离子（Ca²⁺）、碳酸氢根（HCO₃^-）试验组间差异显著（P<0.05）,试验组中,渗透压和Na⁺均在6 h下降至最低且显著低于对照组,随后在该水平上波动;催乳素于6 h时下降至最低,24 h时逐渐稳定上升,显著高于对照组（48 h）;K⁺逐渐降低,48 h达到最低值且显著低于对照组;Cl^–在6 h达到最低值且显著低于对照组。结果表明,低盐胁迫下黄鳍金枪鱼幼鱼会受急性低盐的影响,6 h以内渗透压可以保持稳定,之后快速下降并逐渐稳定;催乳素指标受其影响,短时间会降低,但长期胁迫会促进其上升;主要离子K⁺浓度组间差异不显著（P>0.05）,Na⁺和Cl^–浓度各组间存在显著差异（P<0.05）。

关键词: 黄鳍金枪鱼, 急性低盐胁迫, 血清渗透压, 催乳素, 离子浓度

Abstract: In order to study the effects of acute low salinity stress on osmoregulation of juvenile Thunnus albacares, juvenile Thunnus albacares were transferred from salinity 34 to salinity 29（experimental group）and salinity 34（control group）, and sampled at 0 h, 6 h, 24 h and 48 h after transfer to analyze the effects of acute low salt stress on serum osmotic pressure, prolactin（PRL）lactic acid, blood sugar and ion concentration of juvenile Thunnus albacares. The results showed that the data of osmotic pressure, serum lactic acid, blood glucose, sodium ion（Na⁺）, potassium ion（K⁺）, chloride ion（Cl^-）, calcium ion（Ca²⁺）, and bicarbonate（HCO₃^-）were significantly different between the experimental groups（P<0.05）. In the experimental group, osmolality and Na⁺ decreased to the lowest level at 6 h and were significantly lower than those in the control group, and then fluctuated at this level. Prolactin decreased to the lowest level at 6 h and gradually increased steadily at 24 h, which was significantly higher than that in the control group（48 h）. K⁺ gradually decreased and reached the lowest value at 48 h, which was significantly lower than that of the control group. Cl^– reached the lowest value at 6 h and was significantly lower than that in the control group. The results showed that the osmotic pressure of juvenile Thunnus albacares under low salt stress was affected by acute low salt stress, and the osmotic pressure remained stable within 6 h, and then decreased rapidly and gradually stabilized. By its effects, the prolactin index decreased in a short time, but increased rapidly in a long time. There was no significant difference in the concentration of K⁺ among groups（P>0.05）, but there were significant differences in the concentration of Na⁺ and Cl^– among groups（P<0.05）.

Key words: Thunnus albacares, acute low salt stress, serum osmotic pressure, prolactin, ion concentration

中图分类号:

S967.3

张宁璐, 周胜杰, 陈成勋, 于刚, 马振华, 孙金辉. 急性低盐胁迫对黄鳍金枪鱼幼鱼渗透调节影响研究[J]. 天津农学院学报, 2024, 31(4): 55-65.

Zhang Ninglu, Zhou Shengjie, Chen Chengxun, Yu Gang, Ma Zhenhua, Sun Jinhui. Effects of acute low salt stress on osmotic regulation of juvenile yellowfin tuna（Thunnus albacares）[J]. Journal of Tianjin Agricultural University, 2024, 31(4): 55-65.

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急性低盐胁迫对黄鳍金枪鱼幼鱼渗透调节影响研究

Effects of acute low salt stress on osmotic regulation of juvenile yellowfin tuna（Thunnus albacares）

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