天津农学院学报 ›› 2025, Vol. 32 ›› Issue (5): 31-38.doi: 10.19640/j.cnki.jtau.2025.05.006

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SPME-GC-MS结合ROAV法分析藜麦苗和藜麦嫩穗挥发性物质

余琦琪a, 王海凤b, 刘艳彤a, 张睿航a, 曹高燚c, 王俊斌a,通信作者   

  1. 天津农学院 a.基础科学学院,b.农业分析市级实验教学示范中心,c.农学与资源环境学院,天津 300392
  • 收稿日期:2023-11-30 发布日期:2025-11-12
  • 通讯作者: 王俊斌(1979—),男,研究员,博士,研究方向为植物活性物质提取和鉴定。E-mail:419647768@qq.com。
  • 作者简介:余琦琪(2002—),女,本科在读,研究方向为植物活性物质提取和鉴定。E-mail:2963437815@qq.com。
  • 基金资助:
    天津市大学生创新创业训练计划项目(202210061066); 天津市科技计划项目(22ZYCGSN00230); 新疆维吾尔自治区自然科学基金项目(2022D01A10)

SPME-GC-MS combined with ROAV for the analysis of volatile substances of seedlings and tender spikes in quinoa

Yu Qiqia, Wang Haifengb, Liu Yantonga, Zhang Ruihanga, Cao Gaoyic, Wang Junbina,Corresponding Author   

  1. Tianjin Agricultural University, a. College of Basic Science, b. Municipal-level Experimental Teaching Demonstration Center for Agricultural Analysis, c. College of Agronomy and Resource Environment, Tianjin 300392, China
  • Received:2023-11-30 Published:2025-11-12

摘要: 为探究藜麦苗和藜麦嫩穗中关键风味物质的成分,对比分析藜麦苗和藜麦嫩穗关键风味物质的差异,明晰藜麦苗和藜麦嫩穗关键风味物质的感官特征,本研究采用固相微萃取-气相色谱质谱联用法(SPME-GC-MS)分析藜麦苗和藜麦嫩穗中挥发性物质的组成,结合相对气味活度值(ROAV)计算其关键风味物质的构成。结果表明,从藜麦苗和藜麦嫩穗中共鉴定出123种挥发性物质,藜麦苗中醇类物质含量最高(43.54%),藜麦嫩穗中酯类物质含量最高(15.57%);藜麦苗和藜麦嫩穗中挥发性物质差异较大,仅共同含有(E)-3-己烯-1-醇和十六烷这两种化合物,而胺类和醚类物质仅存在于藜麦嫩穗中。ROAV法分析表明:藜麦苗和藜麦嫩穗中挥发性物质种类较多,有利于提高其口感、改善其品质;(E)-3-己烯-1-醇是藜麦苗和藜麦嫩穗中对风味贡献最大的物质;此外,D-柠檬烯、2-乙基己醇、水杨酸甲酯等关键物质也在形成藜麦苗和藜麦嫩穗的独特风味中发挥重要作用。总之,醛类和酯类等关键风味物质使藜麦苗和藜麦嫩穗具有独特的香气和风味。本研究结果可以为进一步开发、利用藜麦苗和藜麦嫩穗提供参考。

关键词: 藜麦, 幼苗, 嫩穗, 挥发性物质, 气相色谱-质谱联用, 相对气味活度

Abstract: In order to investigate and analyze the key aroma substances composition of seedlings and tender spikes in quinoa. The differences of key flavor substances between seedlings and tender spikes in quinoa were compared and analyzed, and the sensory characteristics of key flavor substances between seedlings and tender spikes in quinoa were clarified, solid-phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS)was used to analyze the composition of volatile substances in quinoa seedlings and quinoa spikes in this study, and the relative odor activity value(ROAV)was used to calculate the composition of key aroma substances. The results showed that a total of 123 volatile substances were identified from quinoa seedlings and quinoa spikes. In quinoa seedlings, the content of alcohols is the highest(43.54%). In quinoa spikes, the content of esters is the highest(15.57%). The volatile substances of quinoa seedlings and quinoa spikes differed greatly. Seedlings and tender spikes of quinoa contained only two compounds in common, namely(E)-hexene-1-ol and hexadecane, and amines and ethers existed only in quinoa spikes. The ROAV analysis showed that(E)-3-hexen-1-ol was the most flavor-contributing substance in quinoa seedlings and quinoa spikes. In addition, D-limonene, 2-ethylhexanol, and methyl salicylate, the key aroma substances, played important roles in the formation of distinctive aromas in quinoa seedlings and quinoa spikes, respectively. There are many kinds of volatile substances in the seedings and tender spikes in quinoa, which is beneficial to improve the taste and quality of seedings and tender spikes in quinoa. The key flavor substances, such as aldehydes and esters, make seedings and tender spikes in quinoa have unique aroma and flavor. These results provide reference for the further development and utilization of quinoa seedlings and quinoa spikes.

Key words: quinoa, seedlings, tender spikes, volatile substances, GC-MS, ROAV

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