天津农学院学报 ›› 2025, Vol. 32 ›› Issue (3): 96-102.doi: 10.19640/j.cnki.jtau.2025.03.018

• • 上一篇    

天津市番茄优质种质资源筛选及抗褪绿病研究

黄志昊, 王博, 李冰, 苑瑜瑾通信作者, 王远宏通信作者   

  1. 天津农学院 园艺园林学院,天津 300384
  • 收稿日期:2025-04-11 出版日期:2025-06-30 发布日期:2025-07-02
  • 通讯作者: 王远宏(1974—),男,教授,博士,主要从事植物病理防治和生防微生物分子生物学研究。E-mail:Wangyh@tjau.edu.cn。苑瑜瑾(1993—),女,讲师,博士,主要从事番茄抗病性研究。E-mail:yujinyuan1993@163.com。
  • 作者简介:黄志昊(2000—),男,硕士在读,主要从事抗番茄褪绿病毒育种方面的研究。E-mail:2780272835@qq.com。
  • 基金资助:
    古浪黄花滩移民区蔬菜产业提质增效关键技术集成与示范(23CXNH0014)

Screening of high-quality tomato germplasm resources and study on their disease resistance in Tianjin

Huang Zhihao, Wang Bo, Li Bing, Yuan YujinCorresponding Author, Wang YuanhongCorresponding Author   

  1. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China
  • Received:2025-04-11 Online:2025-06-30 Published:2025-07-02

摘要: 为筛选褪绿病毒高抗优质番茄种质资源,收集了254种番茄种质资源,并通过种子质量、生长势、抗病性、果实产量及品质进行优质种质资源筛选。首先通过发芽率对搜集到的254种种质资源进行筛选,去除发芽率低于80%和发芽势低于60%的种质资源,得到132份种质资源以进行后续研究。此后,通过主成分分析对生长势进行综合排名,选取排名前二十的种质资源进行抗病性分析。在褪绿病毒病的抗病性测定中,‘Ls-693’和‘2023-20-76’的病情指数分别是34.4和37.5,表现为高抗种质(HR),且‘Ls-693’没有出现病害严重度3级和4级的植株,显示出更高的抗病性。果实营养指标方面,‘Ls-693’果实的可溶性糖含量为3.67 %,处于较高水平,酸甜平衡风味好,番茄红素含量达14.96 mg/100 g;‘2023-20-76’可溶性蛋白含量为3.10 mg/g,二者的维生素C含量均在16 mg/100 g左右,处于较高水平。就以上指标而言,‘Ls-693’和‘2023-20-76’总体表现最优,是两个兼具优良表型特征与较强抗病性的番茄种质资源,本结果为后续番茄抗病育种及优质品种推广提供了重要的材料基础。

关键词: 番茄种质资源, 筛选, 抗病性, 果实品质

Abstract: To screen tomato germplasm resources with high resistance to chlorosis virus(ToCV), 243 tomato germplasm resources were collected and screened through seed quality, growth vigor, disease resistance, fruit yield and quality. First, the collected 254 germplasm resources were screened through germination rate. Germplasm resources with a germination rate lower than 80% and a germination energy lower than 60% were removed, and 132 germplasm resources were obtained for subsequent research. Thereafter, a comprehensive ranking of growth vigor was conducted through principal component analysis, and the top 20 germplasm resources in the ranking were selected for disease resistance analysis. In the disease resistance determination of chlorosis virus disease, the disease indices of ‘Ls-693’ and ‘2023-20-76’ were 34.4 and 37.5 respectively, both exhibiting high resistance(HR). Moreover, no plants of ‘Ls-693’ showed disease severity grades 3 and 4, indicating higher disease resistance. In terms of fruit nutritional indicators, the soluble sugar content of ‘Ls-693’ fruit was 3.67%, which was at a relatively high level, with a balanced sweet and sour taste and good flavor, and the lycopene content reached 14.96 mg/100g. The soluble protein content of ‘2023-20-76’ was 3.10 mg/g, and the vitamin C content of both was approximately 16 mg/100g, which was at a relatively high level. Based on the above indicators, ‘Ls-693’ and ‘2023-20-76’ showed the best overall performance. The two tomato germplasm resources screened in this experiment have both excellent phenotypic characteristics and strong disease resistance, and the experimental results provide an important material basis for subsequent tomato disease-resistant breeding.

Key words: tomato germplasm resources, screening, disease resistance, fruit quality

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