耐药型大肠杆菌引发的仔猪大肠杆菌病的PCR快速鉴定

Journal of Tianjin Agricultural University ›› 2014, Vol. 21 ›› Issue (4): 26-28.

• Researches and Scientific Notes • Previous Articles Next Articles

Rapid PCR Identification of Piglet Colibacillosis Caused by Drug Resistant Type Escherichia coli

WANG Xuan, ZHAO Rui-li^{Corresponding Author}, MA Ji-fei, HU Ye, LU Yao, ZHU Yun-nuan

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China

Received:2014-07-15 Online:2014-12-20 Published:2019-10-21

Abstract

Abstract: In order to investigate the application value of polymerase chain reaction (PCR) technique in detection of pathogenic bacteria in piglets, pigs’ stomach, intestinal contents as disease materials from a pig farm in Tianjin area were expected to isolate and cultivate the pathogenic bacteria and test the drug sensitivity. Universal primes were designed to detect 16S rRNA sequences from isolated bacteria by using PCR method and the results were analyzed by the biological software BLAST. The results show that the pathogenic bacteria was a multiple drug resistant type Escherichia coli. A rapid clinical method of detecting colibacillosis of piglet was found in this study.

Key words: piglet, drug resistance, Escherichia coli, PCR, BLAST analysis

CLC Number:

S858.28

WANG Xuan, ZHAO Rui-li, MA Ji-fei, HU Ye, LU Yao, ZHU Yun-nuan. Rapid PCR Identification of Piglet Colibacillosis Caused by Drug Resistant Type Escherichia coli[J]. Journal of Tianjin Agricultural University, 2014, 21(4): 26-28.

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Rapid PCR Identification of Piglet Colibacillosis Caused by Drug Resistant Type Escherichia coli

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