低温对小球藻固碳效能的影响及转录组学分析

doi:10.19640/j.cnki.jtau.2025.03.001

Journal of Tianjin Agricultural University ›› 2025, Vol. 32 ›› Issue (3): 1-7.doi: 10.19640/j.cnki.jtau.2025.03.001

Physiological response and transcriptomic analysis of Chlorella vulgaris to cold stress

Zhang Rui, Gao Jinwei, Jiang Qingqing, Wang Yuqing, Zhang Dan, Zhou Wenli^{Corresponding Author}

Tianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2025-04-13 Online:2025-06-30 Published:2025-07-02

Abstract

Abstract: In order to investigate the effects of different cryopreservation times on the physiology and biochemistry of Chlorella vulgaris and related mechanisms, C. vulgaris was recovered and re-cultured after 10 d and 90 d of cryopreservation, and the indicators of algal cell growth and carbon sequestration efficacy were analysed, and differential genes and metabolic pathways related to photosynthesis and carbon sequestration efficacy were screened with the help of high-throughput sequencing technology. The results showed that compared with the control group. the growth density of Chlorella recovered to normal on the 8th day of recovery in both the 10 d and 90 d experimental groups, and the carbon sequestration rate was significantly higher than that of the control group on the 8th day（P<0.05）, and the activities of CA and Rubisco were increased; the annotation of the KEGG pathway showed that the differential genes were enriched in the pathways of DNA replication and the Calvin cycle of carbon sequestration in the 10 d experimental group, among which the Calvin cycle carbon sequestration pathway was enriched with 13 DEGs; in the 90 d test group the differential genes were enriched in metabolism-related pathways such as nitrogen metabolism and arginine synthesis.

Key words: Chlorella vulgaris, low temperature, growth, photosynthetic, metabolic pathway

CLC Number:

Q949.91

Zhang Rui, Gao Jinwei, Jiang Qingqing, Wang Yuqing, Zhang Dan, Zhou Wenli. Physiological response and transcriptomic analysis of Chlorella vulgaris to cold stress[J]. Journal of Tianjin Agricultural University, 2025, 32(3): 1-7.

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Physiological response and transcriptomic analysis of Chlorella vulgaris to cold stress

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