人工湿地中异养硝化-好氧反硝化菌分离及脱氮除磷特性的研究

doi:10.19640/j.cnki.jtau.2023.04.008

Journal of Tianjin Agricultural University ›› 2023, Vol. 30 ›› Issue (4): 39-45.doi: 10.19640/j.cnki.jtau.2023.04.008

• Researches and Scientific Notes • Previous Articles Next Articles

Isolation of heterotrophic nitrifying aerobic denitrifying bacteria and characteristics of nitrogen and phosphorus removal in artificial wetlands

Cui Kanli, Zhang Dan, Fang Zhanming, Ren Yuxin, Jiang Ruixuan, Sun Xueliang

Tianjin Key Laboratory of Aquatic Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, China

Received:2022-04-15 Online:2023-08-31 Published:2023-11-01

Abstract

Abstract: In order to improve the removal rate of nitrogen and phosphorus in artificial wetland systems and to enhance the knowledge of heterotrophic nitrifying aerobic denitrifying bacteria species and performance in artificial wetlands, a heterotrophic nitrifying-aerobic denitrifying strain KL1 was isolated from an artificial wetland culture tailwater treatment system to investigate its growth factors and its nitrogen and phosphorus removal performance. The results showed that the optimal growth conditions of strain KL1 were as follows: temperature 30-37 ℃, pH 5-7, carbon source with sodium succinate, C/N of（7∶1）-（7∶18）, and C/N/P of（14∶2∶1）-（7∶1∶4）. During the heterotrophic nitrification-aerobic denitrification process of KL1 strain, the removal rate of NH₄-N was 92%, and the removal rate of TN was 81%. There was no accumulation of NO₃-N, and the accumulation of NO₂-N was 2%. In the denitrification process, the NO₃-N and TN removal rates were up to 95% and 44%, and no accumulation of NH₄-N was found. When NaNO₂ was used as the only nitrogen source, the removal rates of NO₂-N and TN were 56% and 41%, and no NO₃-N accumulation was observed. When NH₄Cl was used as the only nitrogen source, the phosphorus removal rate was up to 31%. It was found that strain KL1 had strong heterotrophic nitrification-aerobic denitrification ability and good denitrification and phosphorus removal effect.

Key words: nitrogen and phosphorus removal, artificial wetland, Pseudomonas spp., heterotrophic nitrification and aerobic denitrification, influence factor of growth

CLC Number:

Cui Kanli, Zhang Dan, Fang Zhanming, Ren Yuxin, Jiang Ruixuan, Sun Xueliang. Isolation of heterotrophic nitrifying aerobic denitrifying bacteria and characteristics of nitrogen and phosphorus removal in artificial wetlands[J]. Journal of Tianjin Agricultural University, 2023, 30(4): 39-45.

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Isolation of heterotrophic nitrifying aerobic denitrifying bacteria and characteristics of nitrogen and phosphorus removal in artificial wetlands

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