一株侧耳属野生菌的分离鉴定及初步驯化栽培

doi:10.19640/j.cnki.jtau.2020.02.006

Journal of Tianjin Agricultural University ›› 2020, Vol. 27 ›› Issue (2): 28-30.doi: 10.19640/j.cnki.jtau.2020.02.006

• Researches and Scientific Notes • Previous Articles Next Articles

Identification and cultivation of a wild Pleurotus ostreatus

WAN An-qi, LIN Xiao-yun, HUANG Liang^{Corresponding Author}, BAN Li-tong, SUN Ning, WANG Yu, LI Yi-ting

College of Agronomy and Resources Environment, Tianjin Agricultural University, Tianjin 300384, China

Received:2019-11-24 Online:2020-06-30 Published:2020-07-03

Abstract

Abstract: The object of this paper was to isolate and identify a wild fungus which was obtained from Baodi district, Tianjin city. The genomic DNA of the wild fungus was extracted and used as templates for the 18S rDNA gene PCR amplification. The 18S rDNA gene fragments were sequenced later. Multiple sequence alignmentwas operated by BLAST in GeneBank. Then other known species with high homology were used for the phylogenetic tree construction. By the 18S rDNA sequence analysis combined with morphological classification, results showed that the fungus belonged to the Basidiomycetes, Agaricales, Pleurotaceae, Pleurotus ostreatus. The fruiting body was cultivated successfully and the average biological efficiency of the first harvest was 50.4%.

Key words: Pleurotus ostreatus, 18S rDNA, phylogenetic trees, artificial cultivation

CLC Number:

S646

WAN An-qi, LIN Xiao-yun, HUANG Liang, BAN Li-tong, SUN Ning, WANG Yu, LI Yi-ting. Identification and cultivation of a wild Pleurotus ostreatus[J]. Journal of Tianjin Agricultural University, 2020, 27(2): 28-30.

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Identification and cultivation of a wild Pleurotus ostreatus

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